Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   GVI46_RS19445 Genome accession   NZ_CP047699
Coordinates   3793500..3793874 (+) Length   124 a.a.
NCBI ID   WP_223269005.1    Uniprot ID   -
Organism   Priestia megaterium strain 5-3     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3788500..3798874
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GVI46_RS19425 - 3788991..3789662 (-) 672 WP_013058558.1 response regulator transcription factor -
  GVI46_RS19430 - 3789988..3790806 (+) 819 WP_013058559.1 squalene/phytoene synthase family protein -
  GVI46_RS19435 - 3790842..3792791 (-) 1950 WP_053487648.1 FUSC family protein -
  GVI46_RS19440 - 3792947..3793207 (-) 261 WP_013058561.1 metal-sensitive transcriptional regulator -
  GVI46_RS19445 nucA/comI 3793500..3793874 (+) 375 WP_223269005.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  GVI46_RS19450 comJ 3793910..3794317 (+) 408 WP_013058563.1 competence protein ComJ -
  GVI46_RS19455 helD 3794346..3796664 (-) 2319 WP_013058564.1 RNA polymerase recycling motor HelD -
  GVI46_RS27400 - 3796909..3797034 (-) 126 WP_016765178.1 hypothetical protein -
  GVI46_RS19460 - 3797174..3797692 (-) 519 WP_223272391.1 enoyl-CoA hydratase/isomerase family protein -
  GVI46_RS19465 - 3797932..3798300 (+) 369 WP_029323759.1 winged helix-turn-helix transcriptional regulator -

Sequence

Protein


Download         Length: 124 a.a.        Molecular weight: 13661.90 Da        Isoelectric Point: 4.2601

>NTDB_id=415506 GVI46_RS19445 WP_223269005.1 3793500..3793874(+) (nucA/comI) [Priestia megaterium strain 5-3]
MGHAAFLAPEKTEAEVNYDATLYFPLDRYPETGDHIRDAIAAGHSSVCTIDRDGAEANREESLKGYPTKTGYDRDEWPMA
MCEEGGAGADIRYISPSDNRGAGSWVGNQLEQYPDGTRVQFIVQ

Nucleotide


Download         Length: 375 bp        

>NTDB_id=415506 GVI46_RS19445 WP_223269005.1 3793500..3793874(+) (nucA/comI) [Priestia megaterium strain 5-3]
ATGGGACATGCAGCTTTTTTAGCTCCTGAAAAAACGGAAGCTGAAGTAAACTATGATGCGACGCTTTATTTTCCGTTAGA
CCGATATCCTGAAACGGGTGATCATATTCGTGACGCGATTGCCGCTGGGCACTCTTCCGTTTGCACAATTGATCGCGACG
GTGCTGAAGCGAACCGGGAAGAATCGCTGAAAGGATATCCGACAAAAACAGGCTATGACCGTGATGAATGGCCAATGGCT
ATGTGCGAAGAAGGCGGAGCAGGAGCTGACATTCGCTATATTTCACCTTCTGATAACCGTGGAGCCGGCTCATGGGTTGG
AAATCAGTTAGAACAGTATCCAGATGGTACACGCGTACAATTTATCGTTCAATAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(30-120)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

67.227

95.968

0.645

Multiple sequence alignment