Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   GVI46_RS16395 Genome accession   NZ_CP047699
Coordinates   3252813..3253235 (+) Length   140 a.a.
NCBI ID   WP_013057949.1    Uniprot ID   A0A8D3X0F8
Organism   Priestia megaterium strain 5-3     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3247813..3258235
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GVI46_RS16370 - 3248052..3248873 (+) 822 WP_053487481.1 sensor histidine kinase -
  GVI46_RS16375 - 3248874..3249515 (+) 642 WP_160203605.1 response regulator -
  GVI46_RS16380 - 3249691..3250683 (+) 993 WP_029322907.1 glycerophosphodiester phosphodiesterase family protein -
  GVI46_RS16385 - 3250796..3252178 (+) 1383 WP_097812227.1 PepSY-associated TM helix domain-containing protein -
  GVI46_RS16390 - 3252208..3252672 (+) 465 WP_029322913.1 FixH family protein -
  GVI46_RS16395 nucA/comI 3252813..3253235 (+) 423 WP_013057949.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  GVI46_RS16400 - 3253389..3253742 (+) 354 WP_223272376.1 DUF1516 family protein -
  GVI46_RS16405 - 3253783..3254376 (-) 594 WP_013057951.1 hypothetical protein -
  GVI46_RS16410 - 3254532..3255188 (-) 657 WP_013057952.1 TetR/AcrR family transcriptional regulator -
  GVI46_RS16415 - 3255212..3256492 (-) 1281 WP_231101755.1 glycosyltransferase -
  GVI46_RS16420 - 3256790..3257335 (+) 546 WP_057273558.1 GNAT family N-acetyltransferase -
  GVI46_RS16425 - 3257643..3258128 (-) 486 WP_053487488.1 hypothetical protein -

Sequence

Protein


Download         Length: 140 a.a.        Molecular weight: 15329.17 Da        Isoelectric Point: 4.4990

>NTDB_id=415501 GVI46_RS16395 WP_013057949.1 3252813..3253235(+) (nucA/comI) [Priestia megaterium strain 5-3]
MKKIVSGILILVLAVIVMIEGNPLEQNSSTNTSYDEVITFPADRYPETAAHIQHAIDKGKSAVCTIDRDGAEENRRESLK
GIPTKSGYDRDEFPMAFCEEGGSGADIEYVKPSDNRGAGSWISHQVDEFPDGTKVLIEVK

Nucleotide


Download         Length: 423 bp        

>NTDB_id=415501 GVI46_RS16395 WP_013057949.1 3252813..3253235(+) (nucA/comI) [Priestia megaterium strain 5-3]
TTGAAAAAAATTGTTTCTGGAATTTTAATTTTAGTATTAGCCGTTATTGTTATGATTGAAGGAAATCCGTTAGAGCAGAA
CTCTTCAACGAATACCTCCTACGATGAAGTAATTACCTTCCCTGCTGATCGTTACCCTGAAACTGCAGCGCACATTCAAC
ATGCAATCGATAAAGGAAAATCGGCTGTTTGTACAATCGACCGAGACGGGGCTGAGGAAAACCGCCGTGAGTCTTTAAAA
GGCATTCCGACTAAAAGCGGCTATGACCGAGATGAGTTTCCGATGGCCTTTTGTGAAGAAGGCGGAAGCGGTGCCGATAT
TGAATATGTAAAGCCTTCAGACAATCGAGGCGCCGGGTCTTGGATCTCACATCAAGTAGACGAGTTTCCTGACGGTACGA
AGGTTTTAATTGAAGTGAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(43-137)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

61.207

82.857

0.507

Multiple sequence alignment