Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   GV218_RS04945 Genome accession   NZ_CP047682
Coordinates   1066551..1067573 (+) Length   340 a.a.
NCBI ID   WP_047570543.1    Uniprot ID   -
Organism   Serratia marcescens strain 3024     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1061551..1072573
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GV218_RS26640 (GV218_04910) - 1062240..1062326 (+) 87 Protein_948 DUF2442 domain-containing protein -
  GV218_RS04915 (GV218_04915) hemW 1062363..1063505 (-) 1143 WP_047570533.1 radical SAM family heme chaperone HemW -
  GV218_RS04920 (GV218_04920) - 1063498..1064091 (-) 594 WP_033635946.1 XTP/dITP diphosphatase -
  GV218_RS04925 (GV218_04925) yggU 1064109..1064399 (-) 291 WP_004948014.1 DUF167 family protein YggU -
  GV218_RS04930 (GV218_04930) - 1064396..1064950 (-) 555 WP_033635944.1 YggT family protein -
  GV218_RS04935 (GV218_04935) proC 1064982..1065803 (-) 822 WP_033635943.1 pyrroline-5-carboxylate reductase -
  GV218_RS04940 (GV218_04940) - 1065823..1066530 (-) 708 WP_047570540.1 YggS family pyridoxal phosphate-dependent enzyme -
  GV218_RS04945 (GV218_04945) pilT 1066551..1067573 (+) 1023 WP_047570543.1 type IV pilus twitching motility protein PilT Machinery gene
  GV218_RS04950 (GV218_04950) ruvX 1067570..1067992 (-) 423 WP_015379046.1 Holliday junction resolvase RuvX -
  GV218_RS04955 (GV218_04955) - 1067992..1068555 (-) 564 WP_033635940.1 YqgE/AlgH family protein -
  GV218_RS04960 (GV218_04960) gshB 1068698..1069648 (-) 951 WP_033654664.1 glutathione synthase -
  GV218_RS04965 (GV218_04965) rsmE 1069659..1070390 (-) 732 WP_033649590.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  GV218_RS04970 (GV218_04970) endA 1070460..1071155 (-) 696 WP_041036666.1 deoxyribonuclease I -
  GV218_RS04975 (GV218_04975) - 1071257..1071769 (-) 513 WP_016930082.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 340 a.a.        Molecular weight: 36407.65 Da        Isoelectric Point: 6.8349

>NTDB_id=415277 GV218_RS04945 WP_047570543.1 1066551..1067573(+) (pilT) [Serratia marcescens strain 3024]
MDIDEFVALSVKHNASDLHLCAGHPPMLRIDGELQPLAAAPTLTAQGVQALCDGLLNAQQRESLRRLGQVDLALHRPGGE
RLRANVFQQSAGMSLALRRIAGQAPSLAELAAPAIVPALLRRDDGLLLVTGATGSGKSTTLAAMIDEINRHQPRHILTLE
DPIEFLHRSRRSLIQQREIGRDSQSFDAALRAALREDPDVILLGELRDIATIRLALTAAETGHLVLATLHTRSAPQAVER
LVDVFPAEEKPYVRAQLAGSLQAVIAQKLMRRPGGGRVAIFEVLTATAAVSNLIREGKTHQLASVLQTGAQSGMQTFEQG
LQQRIDAGVLGESAGEGTEG

Nucleotide


Download         Length: 1023 bp        

>NTDB_id=415277 GV218_RS04945 WP_047570543.1 1066551..1067573(+) (pilT) [Serratia marcescens strain 3024]
ATGGATATCGATGAATTCGTGGCCCTTAGTGTAAAGCATAATGCTTCCGATCTGCACCTTTGTGCCGGTCATCCGCCGAT
GTTGCGCATCGACGGTGAACTGCAGCCGCTGGCGGCGGCGCCAACGCTGACGGCGCAAGGCGTGCAGGCCCTCTGCGACG
GATTGCTGAATGCGCAGCAGCGCGAGAGCCTGCGGCGGCTGGGGCAGGTCGATCTGGCGTTGCACCGGCCGGGCGGGGAG
CGGCTGCGGGCCAACGTTTTTCAGCAAAGCGCGGGGATGTCTCTCGCATTGCGGCGCATCGCCGGGCAGGCGCCTTCGCT
TGCCGAGCTGGCGGCGCCGGCCATCGTCCCGGCGCTGCTGCGGCGCGACGACGGGCTTCTCCTGGTCACCGGCGCCACCG
GCAGCGGCAAGTCCACCACGCTGGCGGCGATGATCGACGAGATCAACCGGCACCAGCCGCGGCACATTTTGACGCTGGAG
GATCCGATCGAATTCCTGCACCGCAGCCGGCGTTCGCTGATCCAGCAGCGGGAAATCGGCCGCGACAGTCAAAGCTTCGA
TGCGGCGTTGCGCGCCGCGCTGCGCGAAGATCCGGATGTGATCCTGCTGGGCGAACTGCGCGATATTGCCACCATTCGGC
TGGCGCTCACCGCGGCGGAGACCGGCCACCTGGTGCTGGCGACGCTGCACACCCGCAGCGCGCCGCAGGCGGTGGAACGG
CTGGTGGACGTGTTTCCGGCGGAAGAGAAACCCTATGTGCGCGCCCAGCTGGCCGGCAGCCTGCAGGCGGTGATCGCGCA
AAAGCTGATGAGGCGGCCCGGCGGCGGGCGAGTGGCGATCTTTGAGGTGCTGACGGCGACGGCGGCGGTCAGCAACCTGA
TCCGCGAAGGGAAAACCCATCAGTTGGCGAGCGTGTTGCAAACCGGCGCGCAGTCCGGCATGCAAACCTTTGAGCAAGGT
TTGCAGCAGCGGATCGACGCCGGCGTGTTGGGGGAGAGTGCGGGGGAGGGAACGGAGGGTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(13-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

54.545

97.059

0.529
  pilT Vibrio cholerae strain A1552

54.545

97.059

0.529
  pilT Acinetobacter baylyi ADP1

51.36

97.353

0.5
  pilT Neisseria gonorrhoeae MS11

50.149

98.529

0.494
  pilT Neisseria meningitidis 8013

50.755

97.353

0.494
  pilT Pseudomonas aeruginosa PAK

49.555

99.118

0.491
  pilT Acinetobacter baumannii strain A118

50.606

97.059

0.491
  pilT Acinetobacter baumannii D1279779

50.606

97.059

0.491
  pilT Acinetobacter nosocomialis M2

50.606

97.059

0.491
  pilT Pseudomonas stutzeri DSM 10701

48.961

99.118

0.485
  pilT Legionella pneumophila strain Lp02

49.398

97.647

0.482
  pilT Legionella pneumophila strain ERS1305867

49.398

97.647

0.482
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.82

100

0.459
  pilU Vibrio cholerae strain A1552

39.877

95.882

0.382
  pilU Acinetobacter baylyi ADP1

39.938

95

0.379
  pilU Pseudomonas stutzeri DSM 10701

38.889

95.294

0.371

Multiple sequence alignment