Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   KNP414_RS20120 Genome accession   NC_015690
Coordinates   4708163..4708588 (-) Length   141 a.a.
NCBI ID   WP_013918144.1    Uniprot ID   F8F962
Organism   Paenibacillus mucilaginosus KNP414     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 4705435..4716284 4708163..4708588 within 0


Gene organization within MGE regions


Location: 4705435..4716284
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KNP414_RS20110 (KNP414_04456) - 4705435..4706331 (-) 897 WP_013918141.1 DMT family transporter -
  KNP414_RS20115 (KNP414_04458) - 4706850..4708094 (+) 1245 WP_013918143.1 hypothetical protein -
  KNP414_RS20120 (KNP414_04459) nucA/comI 4708163..4708588 (-) 426 WP_013918144.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  KNP414_RS20125 (KNP414_04460) - 4708654..4709109 (-) 456 WP_013918145.1 MarR family winged helix-turn-helix transcriptional regulator -
  KNP414_RS20130 - 4709262..4710182 (+) 921 WP_041617142.1 SDR family NAD(P)-dependent oxidoreductase -
  KNP414_RS20135 - 4710132..4711223 (-) 1092 WP_236628495.1 vWA domain-containing protein -
  KNP414_RS20140 - 4711235..4712344 (-) 1110 WP_041617144.1 AAA family ATPase -
  KNP414_RS20145 (KNP414_04462) - 4712369..4712848 (-) 480 WP_014370591.1 DUF6530 family protein -
  KNP414_RS20150 (KNP414_04463) - 4713092..4714009 (-) 918 WP_013918147.1 alpha/beta hydrolase -
  KNP414_RS20155 (KNP414_04464) - 4714140..4714991 (+) 852 WP_013918148.1 helix-turn-helix transcriptional regulator -
  KNP414_RS20160 (KNP414_04465) - 4715055..4715612 (-) 558 WP_013918149.1 NADAR family protein -
  KNP414_RS20165 (KNP414_04466) - 4715637..4716284 (-) 648 WP_013918150.1 HAD family hydrolase -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15759.96 Da        Isoelectric Point: 4.4605

>NTDB_id=41492 KNP414_RS20120 WP_013918144.1 4708163..4708588(-) (nucA/comI) [Paenibacillus mucilaginosus KNP414]
MKKVRLIVQMIFFLVLAIVVFQVLGEQDTQQPDGVDYTLILPADRYPETLKHIREAIAEGESAVCTIDREGAEENRKESL
KGIPTKKGYDRDEWPMAMCAEGGTGADVEYVPFADNRGAGSWIGNQLEQFPNGSRILFQLP

Nucleotide


Download         Length: 426 bp        

>NTDB_id=41492 KNP414_RS20120 WP_013918144.1 4708163..4708588(-) (nucA/comI) [Paenibacillus mucilaginosus KNP414]
ATGAAGAAGGTGCGCTTGATCGTTCAGATGATATTTTTTCTCGTCCTTGCTATTGTTGTATTTCAAGTTCTTGGAGAGCA
GGATACACAGCAGCCAGACGGCGTAGACTATACGCTGATTCTGCCGGCGGACCGGTATCCCGAGACGCTGAAGCATATCC
GGGAGGCTATAGCCGAAGGGGAATCGGCCGTATGCACGATTGACCGGGAGGGGGCGGAGGAGAACCGCAAAGAATCGCTG
AAAGGCATTCCGACGAAAAAGGGCTATGATCGGGATGAGTGGCCTATGGCGATGTGTGCCGAGGGCGGCACCGGTGCGGA
TGTAGAGTATGTGCCCTTCGCGGATAATCGGGGCGCAGGTTCGTGGATCGGCAATCAGCTGGAGCAGTTCCCGAACGGAA
GCCGGATCTTATTTCAATTGCCATAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(46-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB F8F962

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

81.56

0.511

Multiple sequence alignment