Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   GNE05_RS09105 Genome accession   NZ_CP047644
Coordinates   1861036..1861470 (+) Length   144 a.a.
NCBI ID   WP_160182682.1    Uniprot ID   -
Organism   Bacillus sp. AM1(2019)     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1856036..1866470
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GNE05_RS09100 (GNE05_09100) - 1859536..1860747 (-) 1212 WP_160182681.1 cytochrome P450 -
  GNE05_RS09105 (GNE05_09105) nucA/comI 1861036..1861470 (+) 435 WP_160182682.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  GNE05_RS09110 (GNE05_09110) - 1861530..1862285 (+) 756 WP_160182683.1 YoaK family protein -
  GNE05_RS09115 (GNE05_09115) - 1862319..1862681 (-) 363 WP_007410383.1 hypothetical protein -
  GNE05_RS09120 (GNE05_09120) - 1862874..1864202 (-) 1329 WP_160182684.1 S8 family peptidase -
  GNE05_RS09125 (GNE05_09125) - 1864381..1864614 (+) 234 WP_015239897.1 hypothetical protein -
  GNE05_RS09130 (GNE05_09130) - 1864870..1865577 (+) 708 WP_007410381.1 poly-gamma-glutamate hydrolase family protein -
  GNE05_RS09135 (GNE05_09135) - 1865637..1866089 (+) 453 WP_007410380.1 OsmC family protein -
  GNE05_RS09140 (GNE05_09140) - 1866103..1866456 (-) 354 WP_007410379.1 multidrug efflux SMR transporter -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15598.56 Da        Isoelectric Point: 6.7110

>NTDB_id=414872 GNE05_RS09105 WP_160182682.1 1861036..1861470(+) (nucA/comI) [Bacillus sp. AM1(2019)]
MNAFMKWAASLLLVISLQFGLTGAGIHSDSAARAASRYDQVLYFPLSKYPETGNHIKDAISEGHSEICTIDRGGAENRRK
ESLKGIPTKPGFDRDEWPMAVCIEGGAGADIRYVTPSDNRGAGSWVGNQMSGYSDGTRVLFIVQ

Nucleotide


Download         Length: 435 bp        

>NTDB_id=414872 GNE05_RS09105 WP_160182682.1 1861036..1861470(+) (nucA/comI) [Bacillus sp. AM1(2019)]
ATGAATGCGTTTATGAAATGGGCGGCAAGTCTGCTTTTGGTGATCTCTCTTCAGTTCGGCCTTACGGGTGCCGGCATCCA
TTCGGACAGTGCTGCTCGTGCCGCATCCCGATACGATCAGGTGCTGTATTTCCCGCTGTCAAAATATCCGGAGACGGGAA
ATCATATAAAAGACGCCATTTCGGAAGGTCATTCTGAGATTTGTACAATTGATCGGGGCGGAGCGGAGAATAGAAGGAAA
GAATCATTGAAAGGAATTCCGACAAAGCCTGGGTTTGACCGTGATGAATGGCCCATGGCAGTTTGCATAGAAGGCGGGGC
GGGGGCTGATATCAGATATGTAACCCCGTCGGATAACCGCGGGGCCGGTTCATGGGTTGGAAATCAAATGAGCGGATACT
CCGACGGCACGAGAGTACTATTTATCGTTCAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-138)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

57.258

86.111

0.493

Multiple sequence alignment