Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   GNE05_RS00585 Genome accession   NZ_CP047644
Coordinates   106642..108021 (+) Length   459 a.a.
NCBI ID   WP_160182330.1    Uniprot ID   -
Organism   Bacillus sp. AM1(2019)     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 101642..113021
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GNE05_RS00565 (GNE05_00565) ctsR 101993..102457 (+) 465 WP_003156396.1 transcriptional regulator CtsR -
  GNE05_RS00570 (GNE05_00570) - 102471..103028 (+) 558 WP_007410387.1 UvrB/UvrC motif-containing protein -
  GNE05_RS00575 (GNE05_00575) - 103028..104119 (+) 1092 WP_003156398.1 protein arginine kinase -
  GNE05_RS00580 (GNE05_00580) clpC 104116..106548 (+) 2433 WP_007410388.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  GNE05_RS00585 (GNE05_00585) radA 106642..108021 (+) 1380 WP_160182330.1 DNA repair protein RadA Machinery gene
  GNE05_RS00590 (GNE05_00590) disA 108025..109107 (+) 1083 WP_007615225.1 DNA integrity scanning diadenylate cyclase DisA -
  GNE05_RS00595 (GNE05_00595) - 109221..110321 (+) 1101 WP_003156403.1 PIN/TRAM domain-containing protein -
  GNE05_RS00600 (GNE05_00600) ispD 110334..111032 (+) 699 WP_011996188.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  GNE05_RS00605 (GNE05_00605) ispF 111025..111501 (+) 477 WP_003156407.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 49518.00 Da        Isoelectric Point: 7.4337

>NTDB_id=414841 GNE05_RS00585 WP_160182330.1 106642..108021(+) (radA) [Bacillus sp. AM1(2019)]
MAKSKTKFICHSCGYESAKWMGKCPGCGAWNTMVEETIKKAPANRRAAFSHSVQTVQKPSPITSIETSEEPRVHTKLEEF
NRVLGGGVVKGSLVLIGGDPGIGKSTLLLQVSAQLADTAGSVLYISGEESVKQTKLRADRLGINSQMLHVLSETDMEYIS
SAIQEMKPAFVVVDSIQTVYQSDITSAPGSVSQVRECTAELMKIAKTNGIPIFIVGHVTKEGSIAGPRLLEHMVDTVLYF
EGERHHTFRILRAVKNRFGSTNEMGIFEMREEGLTEVLNPSEIFLEERSAGASGSSIVASMEGTRPILVEIQALISPTSF
GNPRRMATGIDHNRVSLIMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAVAVSIASSFRDTPPNPADCFIGEVGLT
GEVRRVSRIEQRVKEAAKLGFKRMIIPVANADGWTIPKGIEVVGVANVAEALRTSLGGS

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=414841 GNE05_RS00585 WP_160182330.1 106642..108021(+) (radA) [Bacillus sp. AM1(2019)]
ATGGCTAAATCAAAAACAAAATTCATCTGCCACTCATGCGGTTATGAATCCGCAAAATGGATGGGAAAGTGCCCGGGCTG
CGGGGCTTGGAATACAATGGTGGAAGAAACCATAAAAAAAGCGCCGGCGAATCGGCGGGCGGCTTTCTCCCACTCCGTTC
AAACCGTCCAGAAACCATCACCCATTACATCAATTGAAACATCGGAAGAACCCCGCGTTCACACAAAGCTCGAAGAATTC
AACAGAGTGCTTGGCGGCGGCGTTGTGAAGGGCTCGTTAGTATTAATCGGCGGCGATCCCGGCATCGGAAAATCAACGCT
CCTTCTCCAAGTATCCGCACAATTAGCAGATACGGCCGGCAGCGTGCTTTACATTTCAGGGGAAGAATCCGTGAAGCAAA
CGAAGCTGCGTGCCGACAGACTTGGAATCAACAGCCAAATGTTACATGTTTTATCTGAAACCGATATGGAGTATATTTCG
TCTGCTATACAAGAGATGAAACCCGCATTTGTTGTCGTAGACTCCATTCAGACCGTGTACCAAAGTGATATCACCTCAGC
TCCCGGCAGTGTATCACAAGTAAGAGAGTGTACGGCCGAATTGATGAAAATAGCGAAAACAAACGGCATACCTATTTTTA
TCGTCGGGCACGTCACAAAAGAAGGATCGATCGCAGGGCCGAGATTGCTTGAGCACATGGTCGATACCGTTCTATACTTT
GAAGGAGAGCGGCACCATACATTCCGTATTTTGCGGGCCGTAAAAAACCGTTTCGGCTCCACAAATGAAATGGGCATCTT
TGAAATGCGGGAAGAGGGGCTTACGGAGGTGCTGAACCCTTCTGAAATATTCTTGGAAGAGCGTTCTGCCGGGGCATCAG
GTTCGAGTATCGTTGCGTCAATGGAAGGAACGAGACCGATCCTGGTTGAAATTCAGGCGCTCATATCACCGACCAGTTTT
GGAAACCCGCGCCGCATGGCTACAGGCATTGACCACAACAGGGTGTCGTTAATTATGGCTGTGCTTGAGAAGCGGGTGGG
TCTTTTGCTTCAAAATCAAGATGCGTATTTAAAGGTCGCCGGCGGGGTCAAACTGGATGAACCGGCGATTGATTTGGCAG
TAGCCGTCAGCATTGCCTCAAGCTTCAGAGATACACCGCCGAATCCCGCAGATTGCTTTATCGGTGAAGTGGGTCTGACA
GGAGAAGTCCGCAGGGTGTCAAGAATCGAACAGCGCGTAAAAGAAGCGGCAAAACTCGGCTTCAAGCGCATGATCATACC
TGTGGCGAATGCAGACGGGTGGACAATACCAAAAGGGATTGAGGTTGTCGGAGTAGCAAATGTTGCAGAAGCGCTTCGTA
CTTCATTAGGAGGATCATAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(73-222)

Subunit ChlI of Mg-chelatase (ChlI)

(364-430)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

93.45

99.782

0.932
  radA Streptococcus pneumoniae Rx1

63.797

98.693

0.63
  radA Streptococcus pneumoniae D39

63.797

98.693

0.63
  radA Streptococcus pneumoniae R6

63.797

98.693

0.63
  radA Streptococcus pneumoniae TIGR4

63.797

98.693

0.63
  radA Streptococcus mitis NCTC 12261

63.797

98.693

0.63
  radA Streptococcus mitis SK321

63.576

98.693

0.627

Multiple sequence alignment