Detailed information    

insolico Bioinformatically predicted

Overview

Name   recF   Type   Machinery gene
Locus tag   GNE05_RS00020 Genome accession   NZ_CP047644
Coordinates   3029..4141 (+) Length   370 a.a.
NCBI ID   WP_021494588.1    Uniprot ID   -
Organism   Bacillus sp. AM1(2019)     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1..9141
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GNE05_RS00005 (GNE05_00005) dnaA 1..1341 (+) 1341 WP_004392913.1 chromosomal replication initiator protein DnaA -
  GNE05_RS00010 (GNE05_00010) dnaN 1526..2662 (+) 1137 WP_021494589.1 DNA polymerase III subunit beta -
  GNE05_RS00015 (GNE05_00015) rlbA 2798..3013 (+) 216 WP_004392910.1 ribosome maturation protein RlbA -
  GNE05_RS00020 (GNE05_00020) recF 3029..4141 (+) 1113 WP_021494588.1 DNA replication/repair protein RecF Machinery gene
  GNE05_RS00025 (GNE05_00025) - 4159..4404 (+) 246 WP_004392908.1 extracellular matrix/biofilm biosynthesis regulator RemA family protein -
  GNE05_RS00030 (GNE05_00030) gyrB 4461..6377 (+) 1917 WP_014720600.1 DNA topoisomerase (ATP-hydrolyzing) subunit B -
  GNE05_RS00035 (GNE05_00035) gyrA 6593..9055 (+) 2463 WP_160182318.1 DNA gyrase subunit A -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 42400.34 Da        Isoelectric Point: 7.0882

>NTDB_id=414837 GNE05_RS00020 WP_021494588.1 3029..4141(+) (recF) [Bacillus sp. AM1(2019)]
MYIQNLELTSYRNYERAELQFENKVNVIIGENAQGKTNLMEAIYVLSMAKSHRTSNDKELIRWDEDYAKIEGRVMKRNGD
IPMQLVISKKGKKGKVNHIEQQKLSQYVGALNTIMFAPEDLNLVKGSPQVRRRFLDMEIGQVSAVYLYDLSLYQKILSQR
NHFLKQLQSRKQTDRTMLDVLTDQLIEAAAKVVAKRLQFTAQLEKWAQPIHSGISRGLEELALKYHTALDVSDPKDLSKI
GNSYQESFSKLKEKEIERGVTLFGPHRDDVLFYVNGRDVQTYGSQGQQRTTALSLKLAEIDLIHEEIGEYPILLLDDVLS
ELDDYRQSHLLHTIQGRVQTFVTTTSVDGIDHDTLHQAGMFRVENGTLVK

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=414837 GNE05_RS00020 WP_021494588.1 3029..4141(+) (recF) [Bacillus sp. AM1(2019)]
TTGTATATCCAAAATTTAGAATTAACATCCTACCGTAATTATGAGCGCGCTGAGCTTCAATTCGAAAACAAGGTGAATGT
GATCATCGGGGAAAATGCCCAAGGCAAAACCAATCTGATGGAAGCCATCTATGTCCTTTCGATGGCAAAATCACACCGCA
CCTCAAATGACAAAGAACTTATACGGTGGGACGAAGACTATGCTAAAATAGAAGGAAGAGTGATGAAGCGCAACGGGGAC
ATCCCGATGCAGCTTGTCATCTCCAAAAAAGGTAAAAAGGGCAAGGTCAATCATATAGAGCAGCAAAAACTCAGTCAGTA
TGTCGGAGCGCTGAACACCATTATGTTTGCCCCTGAGGATTTAAATCTCGTCAAAGGAAGCCCTCAGGTGAGGAGGAGAT
TTCTCGATATGGAGATCGGGCAGGTTTCTGCCGTTTATCTGTATGATTTATCTCTTTATCAAAAAATCCTTTCACAGCGG
AATCATTTTCTGAAACAGCTGCAAAGCCGGAAACAGACTGACCGGACTATGCTTGACGTTCTCACTGATCAGCTGATTGA
AGCAGCCGCAAAAGTTGTCGCCAAACGTCTGCAGTTTACGGCACAGCTTGAAAAATGGGCGCAGCCGATCCATTCCGGCA
TTTCCCGGGGACTTGAAGAACTGGCGCTTAAGTATCACACGGCGCTTGATGTATCAGATCCCAAAGATTTGTCGAAAATA
GGTAACAGCTACCAGGAGTCTTTTTCGAAATTAAAAGAAAAAGAAATAGAACGAGGCGTCACATTATTCGGACCGCATCG
GGATGATGTCCTTTTTTATGTGAACGGACGCGATGTGCAGACGTACGGTTCACAGGGACAGCAGCGTACGACGGCTTTGT
CTTTGAAGCTGGCCGAAATAGATTTGATTCATGAAGAAATCGGAGAATACCCCATTTTACTTTTGGATGATGTACTAAGC
GAGCTGGATGATTACCGACAGTCCCATTTGCTTCATACGATCCAAGGCCGTGTGCAGACATTTGTCACCACGACAAGCGT
GGATGGCATTGACCACGATACCTTACATCAAGCAGGAATGTTCCGTGTGGAAAACGGTACGTTAGTGAAGTGA

Domains

Predicted by InterproScan.

RecF/RecN/SMC N terminal domain (SMC_N)

(2-348)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recF Bacillus subtilis subsp. subtilis str. 168

94.054

100

0.941

Multiple sequence alignment