Detailed information NTG0414740

Bioinformatically predicted

Overview

• Name: comEC
• Type: Machinery gene
• Locus tag: GU333_RS07660
• Genome accession: NZ_CP047630
• Coordinates: 1548470..1550722 (-)
• Length: 750 a.a.
• NCBI ID: WP_245328114.1
• Uniprot ID: -
• Organism: Lactococcus raffinolactis strain Lr_18_12S
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1543470..1555722

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GU333_RS07630 (GU333_07605)	atpA	1543727..1545232 (-)	1506	WP_061773979.1	F0F1 ATP synthase subunit alpha	-
GU333_RS07635 (GU333_07610)	-	1545373..1545900 (-)	528	WP_061773978.1	F0F1 ATP synthase subunit delta	-
GU333_RS07640 (GU333_07615)	atpF	1545903..1546412 (-)	510	WP_061773977.1	F0F1 ATP synthase subunit B	-
GU333_RS07645 (GU333_07620)	atpB	1546425..1547135 (-)	711	WP_096039368.1	F0F1 ATP synthase subunit A	-
GU333_RS07650 (GU333_07625)	-	1547241..1547441 (-)	201	WP_003140734.1	F0F1 ATP synthase subunit C	-
GU333_RS07655 (GU333_07630)	-	1547597..1548391 (-)	795	WP_096039369.1	alpha/beta hydrolase family protein	-
GU333_RS07660 (GU333_07635)	comEC	1548470..1550722 (-)	2253	WP_245328114.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
GU333_RS07665 (GU333_07640)	-	1550688..1551341 (-)	654	WP_245328115.1	helix-hairpin-helix domain-containing protein	-
GU333_RS07670 (GU333_07645)	-	1551399..1552619 (-)	1221	WP_157738471.1	ABC transporter permease	-
GU333_RS07675 (GU333_07650)	-	1552612..1553505 (-)	894	WP_061773972.1	ABC transporter ATP-binding protein	-
GU333_RS07680 (GU333_07655)	-	1553680..1554114 (-)	435	WP_061773971.1	hypothetical protein	-
GU333_RS07685 (GU333_07660)	-	1554170..1554364 (-)	195	WP_061773970.1	helix-turn-helix transcriptional regulator	-

Sequence

Protein

• Length: 750 a.a.
• Molecular weight: 85555.60 Da
• Isoelectric Point: 10.1271

>NTDB_id=414740 GU333_RS07660 WP_245328114.1 1548470..1550722(-) (comEC) [Lactococcus raffinolactis strain Lr_18_12S]
MKNLKKALRWINLPSIFIAYFCVLIYYTVFCFSYLLLGYLIISLLILIWQNGIRTCLKLSMILIGFATICLALQDREAKD
FAREMTVTRISPILDTIQVDGDQVSFRGKSGKQTYQVYYRVTTEQEQRYFKQLAQPVVLSISGQLEKASQQRNFNGFDYQ
HHLKTQNIYRVLTIEQVTGVTRKQTLTLALLRRKAILFSEQHFPRPMSAYMTGLLFGHLGKDFEDMSSIYTSLGIMHLFA
LSGMQVSFFIDFLRKGMLRLGFRRDVVDWFQIPFSVFYAGLTGFSISVNRSLVQKILANFGIKALDNFSLTLLLLFITSP
KFLLTTGGTLSLLFAFVISIFGDRFENLPKYCKLLAESLTLSLSVLPLLILYFHTFQPVSIILTFVFSFLFDVLFLPGLS
VIFLLAMVTGFTLTQVNLIFQWLEDLIKLTDSWWHYPLVLGKPTALVFLAMLVVTGFLIDRWRNRKVSARLAIILMGLFF
ITKNPPTPSMTMVDVGQGDSIFLQDKFNRRNILIDTGGRIELGKKKKWQERNIAAPAEKTLIPYLKSRGVGQIDTLVLTH
THEDHMGDLLAVIDQIKVKNIIVSQGSLNNAQFVKLLKQTRAKVQVAKVGQRLKIFDSYLEVLYPLTPGDGKNNDSIVLY
GKLYGTKFLLTGDLEETGERELLTRYPNLSVDVLKVGHHGSKTSSSESFIEAIRPKVGLISCGLDNRYGHPNMETLTTFQ
KYEVQTFRTDYHGAIKFKKNSKFWHISTVK

Nucleotide

• Length: 2253 bp

>NTDB_id=414740 GU333_RS07660 WP_245328114.1 1548470..1550722(-) (comEC) [Lactococcus raffinolactis strain Lr_18_12S]
TTGAAAAACTTAAAGAAAGCATTACGGTGGATTAATCTACCATCGATTTTCATCGCCTATTTTTGTGTGCTGATCTACTA
TACCGTCTTTTGTTTCTCCTATTTACTTTTAGGTTATCTCATCATTTCTTTGCTAATCTTGATCTGGCAAAATGGCATCA
GAACTTGTTTAAAGCTCAGCATGATCTTAATTGGGTTTGCAACCATTTGTCTAGCGCTACAAGATCGTGAAGCTAAAGAT
TTTGCGCGTGAGATGACAGTGACACGGATCAGCCCCATACTGGATACGATTCAGGTGGATGGTGACCAGGTCTCTTTTCG
TGGGAAATCGGGCAAACAAACCTATCAAGTCTATTATAGGGTCACGACGGAACAAGAGCAGCGCTACTTTAAGCAATTAG
CACAACCAGTCGTCCTATCCATTTCAGGTCAACTAGAAAAAGCGAGTCAACAAAGAAATTTCAATGGCTTTGATTATCAA
CACCATCTCAAGACACAAAATATCTATCGAGTTTTGACGATTGAACAGGTGACAGGGGTGACAAGAAAACAAACCTTAAC
CTTAGCGCTCTTACGACGAAAAGCTATTTTGTTTAGTGAGCAACACTTTCCGCGACCTATGTCAGCCTACATGACCGGTT
TACTTTTTGGGCATCTCGGTAAAGATTTTGAAGATATGAGCAGTATCTATACCAGCTTAGGCATCATGCACCTCTTTGCG
CTATCAGGCATGCAAGTGTCATTTTTTATCGACTTTTTAAGAAAGGGTATGCTCAGACTAGGGTTCAGGAGAGATGTTGT
CGATTGGTTTCAAATCCCTTTTTCTGTTTTTTATGCTGGTCTTACAGGATTTTCCATATCTGTCAACAGATCCTTGGTCC
AGAAAATCCTAGCTAATTTTGGAATTAAGGCGCTTGACAATTTCAGTCTCACGTTGTTGCTTCTATTTATCACGTCGCCA
AAATTCCTTTTAACCACAGGCGGCACCTTGTCTTTACTTTTTGCCTTCGTCATTTCAATCTTTGGTGATCGCTTTGAGAA
CCTCCCAAAATATTGTAAACTACTAGCAGAAAGTTTAACCCTTTCTCTATCCGTTTTGCCGCTCCTCATCCTTTATTTTC
ACACCTTCCAACCGGTGTCGATCATCTTGACCTTTGTCTTCAGCTTTCTTTTTGATGTTCTCTTCCTACCAGGTCTATCC
GTTATTTTCTTGCTAGCAATGGTAACAGGCTTCACGTTGACACAAGTCAATCTCATTTTTCAGTGGCTTGAAGACCTTAT
CAAATTGACAGATAGCTGGTGGCATTATCCGTTAGTACTTGGTAAACCAACAGCCTTGGTCTTTCTGGCAATGCTAGTTG
TGACAGGCTTTTTAATTGACCGCTGGCGAAATCGAAAAGTTAGTGCTAGGCTAGCAATCATCTTGATGGGCTTATTCTTC
ATAACTAAAAATCCACCGACCCCTTCGATGACCATGGTGGACGTTGGCCAGGGGGATAGTATTTTCTTACAAGATAAGTT
TAATCGACGCAATATTTTAATCGATACAGGTGGCAGGATTGAATTAGGTAAGAAGAAAAAGTGGCAGGAGCGTAACATTG
CAGCACCAGCTGAAAAGACTTTAATTCCTTATTTGAAAAGTCGGGGTGTGGGTCAGATTGATACTTTGGTTCTAACCCAT
ACTCACGAGGACCATATGGGTGACCTACTCGCTGTCATCGACCAAATTAAGGTCAAAAATATCATCGTGAGTCAAGGCAG
TCTCAATAATGCCCAATTTGTCAAACTCTTAAAGCAAACCCGAGCTAAGGTTCAAGTGGCCAAGGTCGGTCAACGACTAA
AGATTTTTGACAGCTATTTGGAAGTTCTGTATCCATTGACCCCAGGAGATGGTAAAAATAATGACTCGATCGTCTTGTAT
GGTAAGCTCTATGGGACAAAATTTTTGTTAACAGGTGATTTGGAGGAGACAGGCGAGCGAGAGTTGTTGACACGTTATCC
CAATCTCTCAGTTGATGTTTTAAAAGTAGGGCATCATGGCTCAAAAACGTCATCTTCTGAGTCTTTTATTGAAGCGATTC
GACCAAAAGTCGGATTGATTTCTTGTGGTTTGGATAATCGGTACGGTCATCCAAATATGGAAACTCTCACAACCTTCCAG
AAATATGAGGTTCAAACTTTTCGAACAGATTATCATGGTGCTATAAAATTTAAGAAAAACAGTAAATTTTGGCACATCTC
GACAGTAAAGTGA

Domains

Predicted by InterproScan.

• Domain of unknown function (DUF4131) (DUF4131) (34-178)

• Metallo-beta-lactamase superfamily (Lactamase_B) (495-703)

• Competence protein (Competence) (214-463)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEC	Lactococcus lactis subsp. cremoris KW2	51.822	98.8	0.512
comEC/celB	Streptococcus mitis NCTC 12261	46.817	100	0.471
comEC/celB	Streptococcus mitis SK321	46.235	100	0.467
comEC/celB	Streptococcus pneumoniae TIGR4	45.358	100	0.456
comEC/celB	Streptococcus pneumoniae Rx1	43.857	100	0.443
comEC/celB	Streptococcus pneumoniae D39	43.857	100	0.443
comEC/celB	Streptococcus pneumoniae R6	43.857	100	0.443