Detailed information    

experimental Experimentally validated

Overview

Name   recB   Type   Machinery gene
Locus tag   OK783_RS01905 Genome accession   NZ_CP115654
Coordinates   361247..364849 (-) Length   1200 a.a.
NCBI ID   WP_010951035.1    Uniprot ID   Q5F9M3
Organism   Neisseria gonorrhoeae strain FA1090     
Function   homologous recombination   
Homologous recombination

Function

Mutations in the Gc recB, recC or recD genes revealed that a Gc RecBCD pathway also exists and is involved in DNA transformation and DNA repair but not in pilin antigenic variation.


Genomic Context

Location: 356247..369849
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OK783_RS01885 (OK783_01885) - 357105..358229 (-) 1125 WP_003687777.1 DNA cytosine methyltransferase -
  OK783_RS01890 (OK783_01890) radA 358591..359970 (+) 1380 WP_003690821.1 DNA repair protein RadA Machinery gene
  OK783_RS01895 (OK783_01895) - 360245..360724 (-) 480 WP_003692767.1 DUF302 domain-containing protein -
  OK783_RS01900 (OK783_01900) - 360849..361208 (-) 360 WP_003687781.1 rhodanese-like domain-containing protein -
  OK783_RS01905 (OK783_01905) recB 361247..364849 (-) 3603 WP_010951035.1 exodeoxyribonuclease V subunit beta Machinery gene
  OK783_RS01910 (OK783_01910) - 364926..365834 (-) 909 WP_010357322.1 Dyp-type peroxidase -
  OK783_RS01915 (OK783_01915) - 366119..366946 (+) 828 WP_003706633.1 amino acid ABC transporter substrate-binding protein -
  OK783_RS01920 (OK783_01920) - 366936..367652 (+) 717 WP_003687787.1 amino acid ABC transporter permease -
  OK783_RS01925 (OK783_01925) - 367662..368417 (+) 756 WP_003687790.1 amino acid ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 1200 a.a.        Molecular weight: 134268.77 Da        Isoelectric Point: 5.6207

>NTDB_id=1132 OK783_RS01905 WP_010951035.1 361247..364849(-) (recB) [Neisseria gonorrhoeae strain FA1090]
MSAPIRAFDPLTAPISGTNLIEASAGTGKTYGIAALFTRLIVLEQKSVERVLVVTFTKAATAELKTRLRARLDDVLQVLE
SKEIAELGDDTLSDGIAAYCAEHHEGDTFLPALLEQALQKESRTRLIVRLKAAIGQFDNAAIYTIHGFCQRILRDYAFLC
QAPFDVELTEEDGDRLLVPAQDFWRERVSGDPVLAALAFKRKAVPQTVLAQIRAYLSRPYLNFRRPQADLKQAQRDAETS
WQTVCRLLPELEAGFWRIHPDLNGNSYRKNSFGNLFKELAQKSAAGQLPCLDKDTHERLLKLSSDKLEAGLKKGKTPDAA
VFAELQKLADFGRDLNALEEAEETTMIRLQLDLIEYLNSSLAEMKKSRRERGFDDLLLDVHTALTDNPHAETPARAVAEN
WEIALIDEFQDTDPLQYEIFQKIFIARNRPLFLVGDPKQAIYSFRGADIYAYLQAAEDARHRYTLATNYRSHAALIGSIG
ALFRLKERPFVLENIGYSEVGAARAESRLSPKRPAVQLRWLHENDNEKANKDVLRRRAADYCADEIARALNEASGGRLNF
KDRPLQSGDIAVLVRTHNEAVMISAALKKRQVQSVLLSRESVFASPEAAALSALIGFWLEPRRAGTLRFVLTSGIFGYDA
QQLHDFNQNESEILHWAESARTALDIWQKYGIFAAMQQFSQTHGIETRLLSRNNGRSLTNYFQLLELLAAEDAQNRNPAA
LHKWLRDQISLAGNNGGDNRAIRLESDEDLVKIVTMHASKGLQYPLVYCPFAWDAQDTGPSDWQILHQGANRAELLAKAQ
LSESEQKQYADEEMAERLRLLYVALTRAEEQLNIYAAYSSDTADNPLAYLIEGLPQDSRETVRRTYACEKDGITMLKRNW
RRVADNAPAGTDFTFTEDAPPPAAYRGNADQAAEFAANSIPERGFRFVRHTSFTALSRHTQTPDGGEEDACPSLDAAETS
VPAMPSETPTASDGISIHDFPKGTQAGLCLHEILEDFKFGQAAAEQETLIADKLKKYGFEEIWLPAVAEMAEACRKTPLT
GAYGLSDIPPGCRRPEMGFTLHTEDFGLKRLRDWLARDDIRLPEVCRAAAETLDFHTVNGFLNGFIDMVCQDPDGNICII
DYKSNHLSAYTRQAMDEAVAHQHYYLQALIYAVAAARYFKLRGQPPAAVSVRYLFLRGLDGKGGGVWRWDIDAAALEQIK

Nucleotide


Download         Length: 3603 bp        

>NTDB_id=1132 OK783_RS01905 WP_010951035.1 361247..364849(-) (recB) [Neisseria gonorrhoeae strain FA1090]
ATGTCCGCACCCATCCGAGCATTCGACCCGCTGACCGCCCCCATTTCCGGCACCAACCTGATTGAGGCCTCTGCCGGCAC
CGGCAAAACCTACGGCATTGCCGCCCTGTTTACGCGCCTGATCGTATTGGAACAAAAAAGCGTCGAGCGCGTATTGGTCG
TTACCTTCACCAAAGCCGCCACTGCCGAGCTGAAAACACGCCTGCGCGCGCGTTTGGACGATGTGTTGCAGGTTTTGGAA
AGCAAAGAAATTGCCGAACTTGGAGACGACACGCTTTCAGACGGCATTGCCGCCTACTGCGCCGAACACCACGAAGGCGA
CACCTTCCTGCCCGCACTCTTGGAACAGGCTTTGCAAAAAGAAAGCCGGACGCGTCTGATTGTCCGCCTCAAGGCCGCCA
TCGGGCAATTCGACAACGCCGCCATCTACACCATCCACGGCTTCTGCCAGCGCATCCTGCGCGACTACGCCTTCCTGTGC
CAAGCACCGTTCGATGTCGAACTGACCGAAGAAGACGGCGACCGCCTGCTTGTCCCGGCTCAAGATTTTTGGCGGGAACG
CGTCAGCGGCGACCCGGTGCTTGCCGCATTGGCGTTTAAACGCAAAGCTGTGCCGCAAACCGTCCTTGCCCAAATCCGCG
CCTACCTGTCCCGCCCGTACCTGAATTTCCGCCGTCCGCAGGCGGATTTGAAACAGGCGCAGCGCGACGCCGAAACCTCA
TGGCAAACCGTCTGCCGCCTGCTGCCCGAGCTGGAAGCCGGCTTTTGGCGCATCCATCCCGACCTCAACGGCAACAGTTA
CCGCAAAAACAGCTTCGGCAACCTTTTTAAAGAACTGGCACAAAAATCCGCCGCCGGACAACTGCCCTGTCTGGACAAAG
ACACGCACGAACGGCTCTTGAAACTCTCATCCGACAAACTTGAAGCCGGACTGAAAAAAGGCAAAACGCCCGATGCGGCA
GTATTTGCCGAATTGCAGAAACTGGCAGACTTCGGGCGCGATTTGAACGCACTCGAAGAAGCGGAAGAAACAACAATGAT
CCGGCTGCAACTGGATTTAATCGAATATCTCAACAGCAGCCTTGCCGAAATGAAAAAATCGCGCCGCGAACGCGGTTTCG
ACGACCTGCTGCTCGATGTCCACACCGCGCTGACCGACAATCCGCACGCCGAAACCCCCGCCCGCGCCGTTGCCGAAAAC
TGGGAAATCGCGCTGATCGACGAGTTCCAAGACACCGACCCGCTGCAATACGAAATCTTCCAAAAGATTTTCATCGCCCG
AAACCGCCCGCTGTTCCTCGTCGGCGACCCCAAACAGGCGATTTACAGCTTTCGCGGTGCGGACATTTACGCCTACCTTC
AGGCGGCGGAAGACGCGCGGCACCGCTACACGCTCGCCACCAACTACCGCAGCCACGCCGCGCTTATCGGCAGCATCGGC
GCGCTGTTCCGCCTCAAAGAACGCCCGTTCGTTTTGGAAAACATCGGCTATTCGGAAGTCGGTGCGGCGCGTGCCGAAAG
CAGGTTGTCGCCCAAACGTCCTGCCGTACAGCTCCGTTGGCTGCACGAAAACGACAATGAAAAAGCCAACAAAGACGTTT
TGCGCCGCCGTGCCGCCGACTATTGCGCCGACGAAATCGCCCGCGCGCTCAACGAAGCCTCCGGAGGCCGTCTGAATTTC
AAAGACCGCCCGTTGCAGTCGGGCGATATTGCCGTTCTGGTACGCACGCACAACGAGGCGGTGATGATTTCCGCCGCACT
GAAAAAACGGCAGGTGCAAAGCGTCCTGCTTTCGCGCGAATCCGTGTTCGCCTCGCCCGAAGCCGCCGCCCTGTCCGCGC
TCATCGGCTTCTGGCTCGAACCGCGCCGCGCCGGAACGCTGCGCTTTGTCCTGACAAGCGGCATATTCGGTTATGACGCG
CAGCAATTGCACGACTTCAACCAAAACGAAAGCGAGATTTTGCATTGGGCGGAATCTGCCCGAACCGCGCTCGACATATG
GCAAAAATACGGCATTTTCGCCGCTATGCAGCAGTTTTCCCAAACACACGGCATCGAAACGCGCCTCTTAAGCCGGAACA
ACGGGCGCAGCCTGACCAATTATTTCCAACTGCTCGAACTGCTTGCCGCCGAAGACGCGCAAAACCGCAACCCCGCCGCA
CTGCACAAATGGCTGCGCGATCAAATCAGCCTGGCCGGCAACAACGGCGGCGACAACCGCGCCATCCGTTTGGAAAGCGA
CGAAGATTTGGTCAAAATCGTTACCATGCACGCCTCGAAAGGTTTGCAGTATCCGCTGGTGTACTGCCCGTTTGCGTGGG
ACGCGCAAGATACCGGACCGTCCGACTGGCAAATCCTCCACCAAGGCGCAAACCGGGCCGAACTTTTGGCAAAGGCGCAA
CTGTCGGAAAGCGAACAGAAGCAATACGCCGACGAAGAAATGGCGGAACGCCTGCGCCTGCTTTATGTCGCGCTGACGCG
TGCCGAGGAACAGCTCAACATCTACGCCGCCTATTCTTCCGATACCGCCGACAACCCCCTCGCCTACCTGATCGAAGGCT
TGCCGCAAGACAGCCGCGAAACCGTCCGCCGTACCTATGCGTGTGAAAAAGACGGCATAACGATGCTCAAACGCAACTGG
CGGCGCGTGGCGGACAATGCCCCGGCCGGCACGGATTTCACCTTCACAGAAGATGCGCCGCCCCCTGCCGCCTATCGCGG
CAACGCCGATCAAGCCGCCGAATTTGCCGCAAACAGCATTCCCGAACGCGGATTCCGATTTGTCCGCCACACCAGCTTTA
CCGCCTTAAGCCGCCATACCCAAACGCCCGACGGCGGCGAAGAAGATGCCTGCCCGTCCTTGGATGCCGCCGAAACCTCG
GTGCCGGCGATGCCGTCTGAAACGCCGACGGCTTCAGACGGCATATCGATACACGACTTTCCGAAAGGCACGCAGGCGGG
GCTGTGCCTGCACGAAATTCTTGAAGATTTCAAATTCGGACAAGCAGCCGCCGAACAGGAAACCCTCATTGCCGACAAGC
TGAAAAAATACGGTTTTGAAGAAATATGGCTGCCCGCCGTTGCCGAAATGGCGGAAGCCTGCCGCAAAACGCCGCTGACT
GGGGCATACGGCCTGTCCGACATCCCGCCCGGGTGCCGCCGTCCCGAAATGGGCTTTACCCTCCACACCGAAGACTTCGG
CCTCAAACGGCTGCGCGACTGGCTTGCCCGCGACGACATCAGGCTGCCCGAAGTCTGCCGTGCCGCCGCCGAGACGCTCG
ACTTCCACACCGTCAACGGCTTTTTAAACGGCTTTATCGATATGGTCTGCCAAGACCCCGACGGCAATATCTGCATCATC
GACTACAAATCCAACCACCTATCCGCCTACACGCGGCAGGCGATGGACGAAGCCGTCGCGCACCAGCACTATTACCTTCA
GGCACTGATTTACGCCGTTGCCGCCGCGCGCTACTTCAAACTGCGCGGACAACCGCCCGCCGCCGTTTCCGTCCGCTACC
TGTTTTTGCGCGGATTGGATGGCAAAGGCGGCGGCGTTTGGCGTTGGGACATAGATGCCGCCGCTTTGGAACAGATAAAA
TAA

Domains

Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C)

(463-835)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1069-1178)

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(15-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q5F9M3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] I J Mehr et al. (1998) Differential roles of homologous recombination pathways in Neisseria gonorrhoeae pilin antigenic variation, DNA transformation and DNA repair. Molecular Microbiology 30(4):697-710. [PMID: 10094619]