Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   GTH52_RS13005 Genome accession   NZ_CP047467
Coordinates   2609480..2610847 (+) Length   455 a.a.
NCBI ID   WP_017752971.1    Uniprot ID   W6N1Y3
Organism   Clostridium tyrobutyricum strain L319     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2604480..2615847
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GTH52_RS12985 (GTH52_12910) - 2604821..2605288 (+) 468 WP_017752975.1 CtsR family transcriptional regulator -
  GTH52_RS12990 (GTH52_12915) - 2605341..2605874 (+) 534 WP_017752974.1 UvrB/UvrC motif-containing protein -
  GTH52_RS12995 (GTH52_12920) - 2605878..2606921 (+) 1044 WP_017752973.1 protein arginine kinase -
  GTH52_RS13000 (GTH52_12925) - 2606942..2609380 (+) 2439 WP_017752972.1 AAA family ATPase -
  GTH52_RS13005 (GTH52_12930) radA 2609480..2610847 (+) 1368 WP_017752971.1 DNA repair protein RadA Machinery gene
  GTH52_RS13010 (GTH52_12935) disA 2610863..2611936 (+) 1074 WP_017752970.1 DNA integrity scanning diadenylate cyclase DisA -
  GTH52_RS13015 (GTH52_12940) - 2611961..2612365 (-) 405 WP_017752969.1 hypothetical protein -
  GTH52_RS13020 (GTH52_12945) - 2612647..2613753 (+) 1107 WP_017752968.1 PIN/TRAM domain-containing protein -
  GTH52_RS13025 (GTH52_12950) ispD 2613755..2614456 (+) 702 WP_017752967.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -

Sequence

Protein


Download         Length: 455 a.a.        Molecular weight: 50373.38 Da        Isoelectric Point: 9.0960

>NTDB_id=413576 GTH52_RS13005 WP_017752971.1 2609480..2610847(+) (radA) [Clostridium tyrobutyricum strain L319]
MPKIKTIFVCQDCGYESLKWMGKCPNCSEWNTMVEETKISNKKNDIKRVSVISSNNEPRNINKIKSGEYERYNTGINELN
RVLGGGIVRGSLTLISGAPGIGKSTLLLQTANNIAKKYGKVLYVSGEESEEQIKMRGDRLGVLSDNLYVISETDLSKIES
HIENSNSIFVVIDSIQTLFKDTVSSAPGSVSQVRECANDIMRIGKTRNISFFIVAHITKQGELAGPRVLEHMVDTVLSFE
GERTEEFRILRTVKNRFGTTSEIGVFEMRQKGLLEILNPSQVFLEDTNFQKEGSIVIGVMEGTRPILIEIQALASRTKAV
MPRRTAVGVDNSRLNLILAVLEKKLKIPFYNCDVYVNVVGGLEIEGTFGDLGLALSLVSSVKSLETKLKNLLVIGEIGLT
GEVRPVSFCDRIVSEGKKMGFKNMLIPIRNKGKIKDNDINIIGVSSLKEALNKVF

Nucleotide


Download         Length: 1368 bp        

>NTDB_id=413576 GTH52_RS13005 WP_017752971.1 2609480..2610847(+) (radA) [Clostridium tyrobutyricum strain L319]
GTGCCAAAAATTAAGACTATATTTGTATGCCAGGATTGTGGATACGAATCACTAAAATGGATGGGGAAATGCCCTAATTG
TAGTGAGTGGAATACTATGGTTGAAGAAACTAAAATTTCAAATAAGAAGAATGATATAAAAAGAGTATCTGTTATTAGTA
GTAATAATGAACCAAGAAATATTAATAAAATAAAATCTGGTGAATATGAAAGATATAATACTGGAATAAATGAATTAAAT
AGAGTATTAGGTGGTGGAATAGTAAGGGGATCGCTTACACTTATATCTGGAGCTCCAGGGATAGGAAAATCTACTCTTCT
TCTTCAAACAGCTAACAATATAGCAAAAAAGTATGGTAAAGTGCTTTATGTCTCAGGAGAAGAATCTGAAGAACAAATAA
AAATGAGGGGAGACAGATTAGGAGTATTATCTGATAACCTTTATGTAATTTCTGAGACTGATTTGAGTAAAATAGAATCT
CATATAGAAAATAGTAATTCCATTTTTGTAGTAATAGATTCAATACAAACACTATTTAAAGATACTGTTAGTTCGGCACC
GGGAAGTGTGTCTCAGGTTAGAGAATGCGCAAATGACATTATGCGTATAGGAAAGACAAGAAATATATCATTTTTTATAG
TGGCTCATATTACTAAGCAAGGAGAGCTAGCAGGTCCTAGAGTGCTTGAACATATGGTAGATACAGTTCTCTCATTTGAG
GGTGAAAGAACGGAAGAGTTTAGAATATTAAGAACAGTTAAAAATAGATTTGGAACTACAAGTGAAATTGGAGTATTTGA
GATGAGGCAAAAAGGTCTTTTAGAAATATTAAATCCTTCTCAAGTATTTTTAGAAGATACTAATTTTCAAAAAGAAGGTT
CAATAGTTATAGGTGTAATGGAAGGAACAAGACCTATACTTATAGAAATTCAGGCACTTGCCAGCAGAACTAAAGCCGTT
ATGCCAAGAAGAACTGCTGTTGGAGTAGATAATTCCAGATTGAATTTAATTTTGGCAGTATTGGAGAAAAAATTAAAAAT
ACCATTTTATAATTGTGATGTTTATGTAAATGTTGTTGGTGGGCTTGAAATAGAAGGAACCTTTGGAGATCTAGGTCTTG
CACTTTCACTTGTATCAAGTGTAAAATCTTTGGAAACAAAATTAAAAAATTTATTAGTAATAGGAGAAATTGGACTTACA
GGAGAGGTACGCCCAGTATCATTTTGTGATAGAATTGTAAGTGAGGGCAAAAAAATGGGATTCAAAAATATGTTGATACC
TATTAGAAATAAGGGGAAGATAAAAGATAATGATATAAATATTATTGGTGTATCATCACTTAAGGAAGCATTAAATAAAG
TTTTTTGA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(345-430)

AAA domain (AAA_25)

(63-221)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB W6N1Y3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

51.974

100

0.521
  radA Streptococcus mitis NCTC 12261

47.807

100

0.479
  radA Streptococcus mitis SK321

47.807

100

0.479
  radA Streptococcus pneumoniae Rx1

47.807

100

0.479
  radA Streptococcus pneumoniae D39

47.807

100

0.479
  radA Streptococcus pneumoniae R6

47.807

100

0.479
  radA Streptococcus pneumoniae TIGR4

47.807

100

0.479

Multiple sequence alignment