Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   GTO86_RS01860 Genome accession   NZ_CP047416
Coordinates   407134..408507 (+) Length   457 a.a.
NCBI ID   WP_098046427.1    Uniprot ID   A0A7H9ECJ8
Organism   Limosilactobacillus reuteri strain 1B     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 402134..413507
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GTO86_RS01830 (GTO86_01835) - 402409..403158 (+) 750 WP_085719646.1 amino acid ABC transporter ATP-binding protein -
  GTO86_RS01835 (GTO86_01840) - 403174..403965 (+) 792 WP_085719645.1 transporter substrate-binding domain-containing protein -
  GTO86_RS01840 (GTO86_01845) - 404073..405413 (-) 1341 WP_085719644.1 aminopeptidase C -
  GTO86_RS01845 (GTO86_01850) rpiA 405442..406125 (-) 684 WP_085719643.1 ribose-5-phosphate isomerase RpiA -
  GTO86_RS01850 (GTO86_01855) - 406137..406433 (-) 297 WP_029507893.1 GNAT family N-acetyltransferase -
  GTO86_RS01855 (GTO86_01860) - 406572..407108 (+) 537 WP_035154966.1 dUTP diphosphatase -
  GTO86_RS01860 (GTO86_01865) radA 407134..408507 (+) 1374 WP_098046427.1 DNA repair protein RadA Machinery gene
  GTO86_RS01865 (GTO86_01870) - 408525..409682 (+) 1158 WP_003670546.1 PIN/TRAM domain-containing protein -
  GTO86_RS01870 (GTO86_01875) cysS 410063..411475 (+) 1413 WP_107690467.1 cysteine--tRNA ligase -
  GTO86_RS01875 (GTO86_01880) - 411487..411900 (+) 414 WP_107690468.1 Mini-ribonuclease 3 -
  GTO86_RS01880 (GTO86_01885) rlmB 411890..412639 (+) 750 WP_107690469.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  GTO86_RS01885 (GTO86_01890) rpmG 412705..412854 (+) 150 WP_003666293.1 50S ribosomal protein L33 -
  GTO86_RS01890 (GTO86_01895) secE 412866..413042 (+) 177 WP_003666294.1 preprotein translocase subunit SecE -

Sequence

Protein


Download         Length: 457 a.a.        Molecular weight: 49962.28 Da        Isoelectric Point: 8.6858

>NTDB_id=413406 GTO86_RS01860 WP_098046427.1 407134..408507(+) (radA) [Limosilactobacillus reuteri strain 1B]
MAKIRTQYVCQNCGYNSPRYLGRCPNCGEWNTLVEEQVETSSAPTKKATTTLTGLVAKPQKINEINSTETPRVKTKLNEL
NRVLGGGIVPGSLILIGGDPGIGKSTLLLQVSGQLSDEHHRVLYVSGEESGTQIKMRAERLKVAGDDFYVYPETNMDSIR
DTIRDLKPEYVVIDSVQTMQATDVSSAIGSVSQIREVTAQLMQIAKSNNITIFVVGHVTKGGAIAGPKILEHMVDTVLYF
EGDLHHTYRILRSVKNRFGSTNELGIFEMHTNGLTEVKNPSEIFLEERLHDATGSAVVVSLEGTRPILVEIQALVTPTVY
GNAQRTATGLSRNRVSLIMAVLEKRANLMLQNQDAYLKAAGGVKLDEPAIDLAIAVSIASSYRDKGTKPTDAFVGEVGLT
GEIRRVNRIEQRVAEAEKLGFKRIFIPKNNLKGWKPSTNIQVVGVSTLKEALYLALG

Nucleotide


Download         Length: 1374 bp        

>NTDB_id=413406 GTO86_RS01860 WP_098046427.1 407134..408507(+) (radA) [Limosilactobacillus reuteri strain 1B]
ATGGCAAAAATTCGAACACAATATGTTTGCCAAAACTGTGGTTACAACTCACCACGCTACTTAGGCCGGTGTCCAAATTG
TGGTGAGTGGAATACTTTAGTGGAAGAACAGGTAGAGACCAGTTCTGCACCAACCAAAAAAGCAACTACCACCTTAACCG
GCTTAGTTGCCAAACCACAGAAAATTAATGAGATTAATAGTACAGAGACTCCCCGTGTTAAAACAAAATTGAATGAGTTA
AATCGCGTTTTAGGTGGAGGAATCGTTCCCGGATCACTAATTCTAATTGGAGGAGATCCGGGAATCGGAAAATCGACCCT
CCTTTTACAAGTTTCCGGACAATTAAGTGATGAGCATCACCGGGTTTTATATGTGTCTGGAGAAGAAAGTGGGACACAAA
TTAAAATGCGGGCGGAACGATTAAAAGTTGCTGGGGATGACTTTTATGTATATCCAGAAACGAATATGGATAGTATTCGG
GATACTATTCGTGACCTCAAGCCAGAGTATGTCGTGATTGACTCGGTACAAACGATGCAGGCGACTGATGTAAGTTCAGC
AATTGGTAGTGTATCGCAAATTCGTGAAGTTACCGCCCAGTTAATGCAAATTGCTAAAAGTAATAACATTACGATTTTTG
TCGTTGGACATGTGACAAAGGGCGGTGCAATCGCTGGTCCAAAGATCTTGGAGCACATGGTTGATACAGTCTTGTATTTT
GAAGGAGACCTGCACCATACATATCGTATTTTGCGATCAGTGAAAAATCGATTTGGTTCAACTAACGAGCTTGGTATTTT
TGAAATGCATACAAATGGGCTGACGGAAGTAAAAAATCCATCAGAAATCTTTTTAGAGGAACGATTACATGATGCAACCG
GTTCAGCGGTGGTTGTTTCATTAGAAGGAACACGACCAATTTTAGTTGAAATTCAAGCGCTGGTAACACCGACCGTTTAT
GGAAATGCACAGCGAACTGCTACCGGTTTGAGCCGAAACCGAGTATCATTGATAATGGCGGTATTAGAGAAACGTGCTAA
CCTAATGCTTCAAAATCAAGATGCTTACTTAAAAGCAGCTGGGGGCGTTAAGTTAGATGAGCCAGCAATTGATTTGGCAA
TTGCAGTAAGTATTGCCTCCAGTTATCGGGATAAAGGAACCAAGCCCACCGATGCGTTTGTCGGTGAGGTAGGTTTGACT
GGGGAAATTCGCCGGGTAAACCGAATTGAACAGCGGGTTGCTGAAGCAGAAAAACTTGGCTTTAAGCGAATCTTTATTCC
CAAAAATAATTTAAAGGGTTGGAAACCGTCAACTAATATTCAAGTAGTCGGTGTCTCAACGCTTAAAGAGGCGTTATATT
TAGCGCTGGGGTAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(363-430)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(65-222)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7H9ECJ8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis SK321

66.372

98.906

0.656
  radA Streptococcus mitis NCTC 12261

66.372

98.906

0.656
  radA Streptococcus pneumoniae Rx1

66.15

98.906

0.654
  radA Streptococcus pneumoniae D39

66.15

98.906

0.654
  radA Streptococcus pneumoniae R6

66.15

98.906

0.654
  radA Streptococcus pneumoniae TIGR4

66.15

98.906

0.654
  radA Bacillus subtilis subsp. subtilis str. 168

62.801

100

0.628

Multiple sequence alignment