Detailed information    

insolico Bioinformatically predicted

Overview

Name   pepF   Type   Regulator
Locus tag   GTO85_RS10665 Genome accession   NZ_CP047415
Coordinates   2113127..2114923 (+) Length   598 a.a.
NCBI ID   WP_180861055.1    Uniprot ID   A0A7H9EAY0
Organism   Lactobacillus crispatus strain 1D     
Function   degradation of XIP; competence shut-off (predicted from homology)   
Competence regulation

Genomic Context

Location: 2108127..2119923
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GTO85_RS10640 (GTO85_10650) - 2109022..2109402 (-) 381 WP_005725472.1 hypothetical protein -
  GTO85_RS10645 (GTO85_10655) - 2109547..2110098 (+) 552 WP_005722451.1 GNAT family N-acetyltransferase -
  GTO85_RS10650 (GTO85_10660) - 2110383..2110970 (+) 588 WP_260985922.1 PBECR4 domain-containing protein -
  GTO85_RS10655 (GTO85_10665) - 2110967..2111656 (-) 690 WP_005718174.1 DUF554 domain-containing protein -
  GTO85_RS10660 (GTO85_10670) - 2111721..2112959 (-) 1239 WP_060461574.1 LCP family protein -
  GTO85_RS10665 (GTO85_10675) pepF 2113127..2114923 (+) 1797 WP_180861055.1 oligoendopeptidase F Regulator
  GTO85_RS10670 (GTO85_10680) - 2114982..2115176 (+) 195 WP_065990698.1 hypothetical protein -
  GTO85_RS10675 (GTO85_10685) - 2115325..2115540 (+) 216 WP_005723825.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
  GTO85_RS10680 (GTO85_10690) - 2115544..2115951 (+) 408 WP_180861058.1 type II toxin-antitoxin system death-on-curing family toxin -
  GTO85_RS10685 (GTO85_10695) - 2116015..2116287 (-) 273 WP_013086917.1 bacteriocin immunity protein -
  GTO85_RS10690 (GTO85_10700) - 2116365..2117162 (-) 798 WP_223876341.1 CPBP family intramembrane glutamic endopeptidase -
  GTO85_RS10695 (GTO85_10705) - 2117178..2117375 (-) 198 WP_180861060.1 helix-turn-helix transcriptional regulator -
  GTO85_RS10700 (GTO85_10710) - 2117372..2117638 (-) 267 WP_260985923.1 hypothetical protein -
  GTO85_RS10705 (GTO85_10715) - 2118270..2119388 (+) 1119 WP_180861063.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  GTO85_RS10710 (GTO85_10720) - 2119446..2119922 (+) 477 WP_005718152.1 S-ribosylhomocysteine lyase -

Sequence

Protein


Download         Length: 598 a.a.        Molecular weight: 68055.97 Da        Isoelectric Point: 4.6648

>NTDB_id=413399 GTO85_RS10665 WP_180861055.1 2113127..2114923(+) (pepF) [Lactobacillus crispatus strain 1D]
MAIPNRNEVPEELKWDLTRIFKNDEEWEQAYVAAQEKIDQLGELKGTLAKSGKDLYEGLTKILAVKREVENIYVYATMSS
DVDTANSHYLGYVSRVQSLANQFEAATSFINPEILSIPSDKLAEFKQAEPRLKNYAHYLEMITNKRPHTLPAEQEKLIAD
AGDALGVSENTFNVLTNSDMEYGYVQDDDGNMEQLSDGLYSLLIQSQNRDVRKGAFDTLYATYGQFQNSLASTLSGVVKK
HNYNARVHKYNSAREAALADNGVPVAVYDTLIQEVDSHLDLLHRYVALRKKILGLKDLQMWDMYVPLTGRPSLSYNFEEA
KETAKKALAPLGEDYLKHVDYIFNNRVIDPIESKNKVTGAYSGGAYDTDPYELLNWEDNIDSLYTLVHETGHSVHSWYTR
NTQPYVYGDYPIFVAEIASTTNENILTEYFLDHITDPKTRAFILNYYLDSFKGTLFRQTQFAVFEQFLHEADAKGEPLTA
DTLDDVYGQINQHYYGDSVEPGGNIALEWSRIPHFYYNFYVYQYATGFAAATALANKVVHGTPAERDAYLGFLKAGSSDY
PTEIMKRAGVDMTKPDYLEDAFKTFEKRLNEFENLIEK

Nucleotide


Download         Length: 1797 bp        

>NTDB_id=413399 GTO85_RS10665 WP_180861055.1 2113127..2114923(+) (pepF) [Lactobacillus crispatus strain 1D]
ATGGCGATACCAAACAGAAATGAAGTCCCAGAAGAACTTAAGTGGGACTTGACCCGAATTTTTAAAAATGATGAAGAATG
GGAACAGGCCTATGTAGCGGCCCAAGAAAAAATCGACCAACTTGGTGAATTAAAAGGTACATTAGCAAAATCTGGCAAAG
ATTTGTACGAAGGCTTAACTAAGATTCTGGCCGTTAAGCGCGAGGTGGAAAATATTTATGTTTATGCCACCATGTCTAGC
GATGTGGATACTGCCAATTCACATTATTTAGGCTATGTCAGCCGGGTGCAAAGCTTAGCTAACCAATTCGAAGCCGCTAC
TAGTTTTATTAACCCAGAAATCTTGAGCATCCCTAGTGATAAACTAGCTGAATTTAAGCAAGCTGAGCCAAGACTAAAGA
ATTATGCTCACTACTTGGAAATGATTACTAATAAGCGACCTCATACCCTGCCAGCTGAACAAGAAAAGTTAATCGCTGAT
GCTGGAGATGCTTTAGGCGTTTCCGAAAATACTTTTAACGTTTTAACTAACTCTGACATGGAATACGGCTACGTCCAAGA
TGACGATGGCAACATGGAACAACTATCTGACGGTTTGTACTCATTATTAATTCAATCGCAAAACCGTGACGTCAGAAAAG
GTGCTTTTGATACACTTTATGCCACTTATGGACAGTTCCAAAATTCCTTGGCTTCAACTTTGTCTGGCGTTGTAAAAAAG
CACAACTATAACGCACGTGTGCACAAGTATAATTCAGCTCGTGAAGCTGCTTTAGCCGATAATGGCGTACCCGTAGCAGT
TTATGACACTTTAATCCAGGAAGTTGATTCACACCTAGACTTACTTCACCGTTATGTTGCGCTACGTAAAAAGATCTTAG
GCCTGAAGGATCTGCAAATGTGGGACATGTATGTGCCACTAACCGGAAGACCTTCTCTTTCATACAATTTCGAAGAAGCA
AAAGAGACAGCAAAAAAGGCTTTGGCTCCACTTGGTGAAGACTACTTGAAGCATGTTGACTATATTTTTAATAATCGTGT
CATCGACCCAATAGAATCTAAAAATAAAGTAACTGGTGCCTACTCCGGTGGTGCTTATGATACCGATCCTTATGAGTTGC
TCAACTGGGAAGATAACATCGATTCACTTTATACTTTAGTCCACGAAACTGGTCACTCCGTTCACTCCTGGTACACTCGC
AATACTCAACCTTACGTTTACGGCGACTATCCAATCTTTGTGGCTGAAATTGCTTCAACTACTAATGAAAATATTTTGAC
CGAATATTTCTTAGACCACATTACCGATCCTAAGACGCGGGCCTTTATCTTAAATTACTACCTTGATTCATTTAAAGGCA
CTTTATTCCGTCAAACTCAATTTGCCGTGTTTGAACAATTCTTGCACGAAGCTGATGCTAAGGGAGAACCACTGACTGCT
GACACTTTAGACGACGTTTACGGTCAAATTAATCAGCATTATTACGGCGATAGCGTTGAACCAGGCGGCAATATTGCCCT
TGAATGGTCACGAATTCCACACTTCTACTACAATTTCTATGTGTACCAATATGCGACTGGCTTTGCGGCTGCAACGGCAT
TAGCAAACAAGGTGGTTCATGGAACGCCTGCTGAGAGGGATGCCTACCTTGGTTTCCTTAAAGCTGGTTCAAGTGATTAC
CCAACTGAGATCATGAAGCGCGCCGGCGTGGACATGACCAAGCCAGACTACTTGGAGGATGCATTCAAGACTTTTGAAAA
GCGGTTGAATGAATTTGAGAATTTGATTGAGAAGTAA

Domains

Predicted by InterproScan.

Oligopeptidase F (Peptidase_M3_N)

(113-181)

Peptidase family M3 (Peptidase_M3)

(203-583)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7H9EAY0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pepF Streptococcus salivarius strain HSISS4

51.684

99.331

0.513

Multiple sequence alignment