Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   GTO85_RS01775 Genome accession   NZ_CP047415
Coordinates   343904..345280 (+) Length   458 a.a.
NCBI ID   WP_180861477.1    Uniprot ID   A0A7H9E6G7
Organism   Lactobacillus crispatus strain 1D     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 338904..350280
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GTO85_RS01740 (GTO85_01745) - 338977..339159 (+) 183 WP_005720404.1 hypothetical protein -
  GTO85_RS01745 (GTO85_01750) - 339171..339851 (+) 681 WP_005724199.1 2,3-diphosphoglycerate-dependent phosphoglycerate mutase -
  GTO85_RS01750 (GTO85_01755) - 339962..340897 (+) 936 WP_005724201.1 AAA family ATPase -
  GTO85_RS01755 (GTO85_01760) - 340961..341365 (-) 405 WP_005720409.1 hypothetical protein -
  GTO85_RS01760 (GTO85_01765) pepC 341505..342854 (-) 1350 WP_180861475.1 aminopeptidase C -
  GTO85_RS01765 (GTO85_01770) - 342922..343221 (-) 300 WP_180861476.1 hypothetical protein -
  GTO85_RS01770 (GTO85_01775) - 343353..343904 (+) 552 WP_005720412.1 dUTP diphosphatase -
  GTO85_RS01775 (GTO85_01780) radA 343904..345280 (+) 1377 WP_180861477.1 DNA repair protein RadA Machinery gene
  GTO85_RS01780 (GTO85_01785) gltX 345358..346857 (+) 1500 WP_089147169.1 glutamate--tRNA ligase -
  GTO85_RS01785 (GTO85_01790) cysS 346948..348381 (+) 1434 WP_180861478.1 cysteine--tRNA ligase -
  GTO85_RS01790 (GTO85_01795) - 348374..348817 (+) 444 WP_005724209.1 mini-ribonuclease III -
  GTO85_RS01795 (GTO85_01800) rlmB 348804..349559 (+) 756 WP_180861479.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  GTO85_RS01800 (GTO85_01805) - 349693..350238 (+) 546 WP_180861481.1 sigma-70 family RNA polymerase sigma factor -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 50188.79 Da        Isoelectric Point: 7.1855

>NTDB_id=413390 GTO85_RS01775 WP_180861477.1 343904..345280(+) (radA) [Lactobacillus crispatus strain 1D]
MARVKTQYKCRSCGYISASYLGRCPNCGAWNQFEKETEEVQKRSTKATASRLIQKTGLNEPVKLDKIKAEKEERIVTKSE
ELNRVLGGGIVPGSLVLIGGDPGIGKSTLMLQIMSDLSEKYKVLYVSGEESANQIKLRADRLGVGQSNMLLYPETDMHDI
REQINDVKPDFVVIDSIQTMNEPSLDSMTGSASQVREVTSELMKIAKMDAITVFVIGHVTKEGAIAGPKILEHMVDTVLY
FEGDEHHSYRILHSVKNRFGAANEIGMFEMVNEGLREVTNPSSIFLDQRLPNSTGSAVVVSLEGTRPLLAEIQALVTPTA
FGYAKRTTSGISFNKASLLLAVLEKRGNLMLQNQDVYLTATGGIKLNEPAIDLAIAMAVASSYTDKEISPTDCFVGEVGL
TGEVRRVDKIDARVKEAAKVGFKRIFIPRHNMHSGLKDHGIEVIPVSSIPQALKLVFG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=413390 GTO85_RS01775 WP_180861477.1 343904..345280(+) (radA) [Lactobacillus crispatus strain 1D]
ATGGCTAGAGTAAAAACACAATATAAGTGTCGTTCTTGCGGCTATATTTCGGCAAGTTATTTAGGCCGCTGTCCTAACTG
TGGTGCTTGGAATCAATTTGAAAAAGAAACTGAAGAAGTGCAAAAGCGTTCTACTAAGGCAACTGCTAGTCGTTTGATTC
AGAAGACTGGATTAAATGAACCGGTAAAGCTGGATAAGATTAAAGCTGAAAAAGAAGAACGAATTGTCACTAAATCTGAG
GAATTAAACCGCGTTTTAGGTGGTGGTATTGTGCCAGGCTCACTAGTTTTAATTGGTGGAGATCCCGGAATTGGTAAGTC
AACCTTGATGTTACAAATTATGAGTGACTTATCTGAAAAATATAAGGTGCTTTACGTTTCGGGAGAAGAATCAGCTAACC
AAATCAAATTGCGGGCTGACCGACTGGGTGTAGGTCAAAGTAATATGCTCTTATATCCAGAGACTGACATGCATGATATT
CGCGAACAAATCAATGATGTTAAGCCTGACTTTGTTGTAATCGACTCCATTCAAACCATGAATGAACCAAGTCTTGATTC
GATGACTGGCTCTGCCTCACAGGTTCGTGAAGTAACAAGTGAATTAATGAAAATTGCCAAGATGGATGCCATTACAGTTT
TTGTTATCGGCCACGTTACAAAAGAGGGTGCGATTGCTGGACCTAAGATCTTGGAACATATGGTGGATACCGTTCTCTAT
TTTGAAGGGGATGAACACCATTCTTACCGAATTTTGCATTCTGTTAAAAACCGTTTTGGAGCAGCAAACGAAATTGGAAT
GTTTGAAATGGTTAATGAAGGCTTAAGAGAGGTAACTAATCCATCATCAATTTTTCTGGACCAAAGATTACCTAATTCTA
CTGGCTCTGCTGTGGTGGTATCGCTTGAAGGAACTAGGCCACTTTTAGCAGAAATTCAGGCTTTGGTTACTCCGACAGCT
TTTGGCTATGCTAAAAGGACCACGTCAGGTATTAGTTTTAACAAAGCTTCATTGCTGCTAGCGGTGCTTGAAAAGCGGGG
AAACTTAATGTTGCAGAATCAAGATGTCTATTTGACTGCAACTGGTGGGATTAAATTGAATGAACCAGCAATTGATTTAG
CAATTGCCATGGCAGTTGCATCCAGTTATACCGATAAAGAAATTTCGCCGACAGATTGTTTTGTAGGCGAAGTGGGCTTA
ACTGGTGAAGTTCGCCGAGTTGATAAAATCGATGCACGAGTAAAAGAAGCGGCTAAAGTAGGGTTTAAGCGGATCTTTAT
TCCACGGCATAATATGCATTCTGGATTAAAAGATCATGGCATTGAGGTAATTCCCGTTTCGAGTATTCCCCAAGCTTTAA
AATTAGTTTTTGGCTAG

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(66-223)

Subunit ChlI of Mg-chelatase (ChlI)

(363-431)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-34)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7H9E6G7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

57.549

99.782

0.574
  radA Streptococcus pneumoniae Rx1

57.549

99.782

0.574
  radA Streptococcus pneumoniae D39

57.549

99.782

0.574
  radA Streptococcus pneumoniae R6

57.549

99.782

0.574
  radA Streptococcus pneumoniae TIGR4

57.549

99.782

0.574
  radA Streptococcus mitis SK321

57.549

99.782

0.574
  radA Bacillus subtilis subsp. subtilis str. 168

55.773

100

0.559

Multiple sequence alignment