Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   GTO84_RS02500 Genome accession   NZ_CP047412
Coordinates   548404..549777 (+) Length   457 a.a.
NCBI ID   WP_035149896.1    Uniprot ID   -
Organism   Ligilactobacillus salivarius strain 2D     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 543404..554777
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GTO84_RS02470 (GTO84_02480) - 543620..544564 (+) 945 WP_180858477.1 AEC family transporter -
  GTO84_RS02475 (GTO84_02485) - 544586..545260 (+) 675 WP_003706506.1 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase -
  GTO84_RS02480 (GTO84_02490) - 545430..545789 (-) 360 WP_003702606.1 bacteriocin immunity protein -
  GTO84_RS02485 (GTO84_02495) - 545993..547327 (-) 1335 WP_003700692.1 aminopeptidase C -
  GTO84_RS02490 (GTO84_02500) - 547416..547694 (-) 279 WP_003702618.1 GNAT family N-acetyltransferase -
  GTO84_RS02495 (GTO84_02505) - 547850..548386 (+) 537 WP_003700690.1 dUTP diphosphatase -
  GTO84_RS02500 (GTO84_02510) radA 548404..549777 (+) 1374 WP_035149896.1 DNA repair protein RadA Machinery gene
  GTO84_RS02505 (GTO84_02515) - 549803..550915 (+) 1113 WP_003700688.1 PIN/TRAM domain-containing protein -
  GTO84_RS02510 (GTO84_02520) gltX 551046..552533 (+) 1488 WP_003700687.1 glutamate--tRNA ligase -
  GTO84_RS02515 (GTO84_02525) cysS 552685..554097 (+) 1413 WP_003702615.1 cysteine--tRNA ligase -
  GTO84_RS02520 (GTO84_02530) - 554090..554497 (+) 408 WP_003700685.1 Mini-ribonuclease 3 -

Sequence

Protein


Download         Length: 457 a.a.        Molecular weight: 49736.40 Da        Isoelectric Point: 8.0254

>NTDB_id=413366 GTO84_RS02500 WP_035149896.1 548404..549777(+) (radA) [Ligilactobacillus salivarius strain 2D]
MAKTKTRYVCQNCEYISPRYLGRCPNCGSWNSLVEEIEKKESTTKAQPRVSISGTTVKPKLIDDVSTVETPRFKTNLEEL
NRVLGGGIVPGSLVLIGGDPGIGKSTLLLQVSGQLSTLGKKVLYVSGEESATQIKLRADRLGVHGTELYLYPETDMDSIR
KNIELLKPDFVVIDSVQTMQEPEMTAAVGSVSQIREVTGDLMQIAKTNGITVFVVGHVTKGGAIAGPKILEHMVDTVLYF
EGDQHRTFRILRAVKNRFGSTNEIGIFEMKNGGLSEVANPSEIFLEERLKGATGSAVVASLEGTRPILVELQALVTPTAF
GNAKRTTTGLDHNRVALLMAVLEKRAGLLLQNQDAYLKAAGGVKLDEPAIDLAIAVSLVSSYRDAETRPTDCFIGEVGLT
GEIRRVNRIEQRVVEAAKLGFKRVFLPKNNLDGWMPPKEIEVVGVSTLNQALKLIFS

Nucleotide


Download         Length: 1374 bp        

>NTDB_id=413366 GTO84_RS02500 WP_035149896.1 548404..549777(+) (radA) [Ligilactobacillus salivarius strain 2D]
TTGGCGAAAACTAAGACAAGATATGTTTGTCAAAATTGTGAATATATCTCTCCACGTTATTTAGGACGTTGCCCTAATTG
TGGTTCTTGGAATAGCTTGGTAGAGGAAATAGAGAAAAAGGAAAGTACAACTAAAGCACAACCTAGAGTTAGTATCTCTG
GTACAACAGTGAAACCTAAATTAATTGATGATGTTTCTACTGTAGAAACTCCAAGATTTAAAACTAATTTAGAAGAATTG
AATCGTGTTTTAGGCGGCGGAATTGTTCCCGGATCTTTAGTGTTAATTGGTGGAGATCCAGGTATTGGCAAGTCTACATT
ATTACTACAAGTTTCTGGACAATTATCTACTCTCGGGAAAAAGGTTCTTTATGTTTCTGGGGAAGAAAGTGCAACTCAAA
TTAAATTACGTGCGGATCGTTTAGGAGTTCATGGAACTGAGCTATATTTATATCCTGAAACAGATATGGATAGTATTAGA
AAAAATATTGAACTTTTAAAGCCTGATTTTGTTGTAATCGATTCGGTGCAGACAATGCAGGAGCCAGAAATGACAGCCGC
AGTTGGAAGTGTTTCTCAAATTAGGGAAGTAACTGGAGATTTGATGCAAATTGCGAAAACTAATGGGATAACGGTCTTTG
TTGTAGGACATGTTACTAAAGGTGGAGCAATTGCAGGGCCTAAAATTTTAGAACACATGGTAGATACTGTTCTTTATTTT
GAGGGAGATCAACATAGAACATTTAGAATTTTACGAGCTGTTAAAAATAGATTTGGTTCAACTAATGAAATTGGAATCTT
TGAAATGAAAAATGGAGGATTAAGCGAAGTTGCTAATCCATCAGAGATTTTTCTAGAAGAAAGATTAAAAGGGGCTACGG
GATCAGCTGTCGTTGCTTCTCTTGAAGGAACTAGACCCATATTGGTAGAATTACAAGCACTAGTAACACCGACTGCATTT
GGAAATGCTAAGCGAACAACAACTGGTTTAGATCACAATCGAGTTGCGTTACTGATGGCAGTACTTGAAAAAAGAGCTGG
TTTGTTATTGCAAAATCAGGATGCTTATTTGAAGGCTGCTGGTGGAGTGAAGTTAGATGAGCCAGCAATAGATTTAGCCA
TTGCTGTTAGTTTAGTTTCTAGTTATCGCGATGCAGAAACAAGACCAACTGACTGTTTTATAGGGGAAGTGGGATTAACT
GGTGAAATAAGACGAGTTAATCGTATTGAACAGCGTGTTGTTGAGGCTGCTAAGCTAGGATTTAAGCGAGTATTTTTACC
AAAGAACAACTTAGATGGTTGGATGCCTCCAAAAGAGATTGAAGTAGTAGGCGTAAGTACACTAAATCAAGCATTAAAAC
TAATTTTTAGTTAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(364-430)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(67-222)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

68.49

100

0.685
  radA Streptococcus mitis SK321

68.271

100

0.683
  radA Streptococcus pneumoniae Rx1

68.271

100

0.683
  radA Streptococcus pneumoniae D39

68.271

100

0.683
  radA Streptococcus pneumoniae R6

68.271

100

0.683
  radA Streptococcus pneumoniae TIGR4

68.271

100

0.683
  radA Bacillus subtilis subsp. subtilis str. 168

65.563

99.125

0.65

Multiple sequence alignment