Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   GTO83_RS08930 Genome accession   NZ_CP047410
Coordinates   1778182..1779558 (-) Length   458 a.a.
NCBI ID   WP_180870543.1    Uniprot ID   A0A7L5UJC0
Organism   Lactobacillus sp. 3B(2020)     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1773182..1784558
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GTO83_RS08910 (GTO83_08900) rlmB 1773855..1774604 (-) 750 WP_180870537.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  GTO83_RS08915 (GTO83_08905) - 1774594..1775004 (-) 411 WP_180870538.1 Mini-ribonuclease 3 -
  GTO83_RS08920 (GTO83_08910) cysS 1775006..1776412 (-) 1407 WP_180870540.1 cysteine--tRNA ligase -
  GTO83_RS08925 (GTO83_08915) - 1777058..1778164 (-) 1107 WP_180870541.1 PIN/TRAM domain-containing protein -
  GTO83_RS08930 (GTO83_08920) radA 1778182..1779558 (-) 1377 WP_180870543.1 DNA repair protein RadA Machinery gene
  GTO83_RS08935 (GTO83_08925) - 1779696..1780235 (-) 540 WP_180872172.1 dUTP diphosphatase -
  GTO83_RS08940 (GTO83_08930) - 1780503..1780796 (+) 294 WP_180870544.1 GNAT family N-acetyltransferase -
  GTO83_RS08945 (GTO83_08935) rpiA 1780812..1781492 (+) 681 WP_180870546.1 ribose-5-phosphate isomerase RpiA -
  GTO83_RS08950 (GTO83_08940) - 1781633..1782247 (+) 615 WP_180870548.1 sugar O-acetyltransferase -
  GTO83_RS08955 (GTO83_08945) - 1782295..1783635 (+) 1341 WP_180870550.1 aminopeptidase C -
  GTO83_RS08960 (GTO83_08950) - 1783754..1784479 (+) 726 WP_180870551.1 gamma-glutamyl-gamma-aminobutyrate hydrolase family protein -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49753.11 Da        Isoelectric Point: 8.9734

>NTDB_id=413357 GTO83_RS08930 WP_180870543.1 1778182..1779558(-) (radA) [Lactobacillus sp. 3B(2020)]
MAKARTHYVCQNCGYSSPRFLGRCPNCGKWNTLVEEVEQGQSVNKVQPTTTLTGIVAKPQKINEIDTRKTPRVKTKLNEL
NRVLGGGIVPGSLVLIGGDPGIGKSTLLLQVSGQLSTEGRSVLYVSGEESASQIKMRAQRLEVAGDNFYIYPETNMDSIR
ATIDSLNPEYVVIDSVQTMQATDVTSAIGSVSQIRAVTAQLMQIAKGKNITVFVVGHVTKGGVLAGPKILEHMVDTVLYF
EGDLHHTYRILRAVKNRFGSTNELGIFEMDNDGLKEVANPSEIFLEERLQDATGSAVVVSLEGTRPILVEIQALITPTVF
GNAQRTATGLNRNRVSLIMAVLEKRANLLLQNQDAYLKAAGGVKLDEPAIDLAIAVAIASSYRDRGTQPTDAFVGELGLT
GEVRRVNRIEQRVAEAAKLGFKRIFVPKNNLQGWKTPDNIEVIGVTTISETLKLALGR

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=413357 GTO83_RS08930 WP_180870543.1 1778182..1779558(-) (radA) [Lactobacillus sp. 3B(2020)]
ATGGCAAAAGCACGAACCCATTATGTTTGTCAGAACTGTGGGTACTCATCGCCCCGTTTCTTGGGGCGATGCCCAAATTG
TGGAAAATGGAATACTCTGGTTGAAGAAGTTGAACAAGGTCAATCGGTAAATAAAGTTCAACCAACAACGACTTTAACCG
GCATTGTTGCTAAGCCACAGAAAATCAACGAAATTGATACTCGAAAAACACCTCGGGTAAAAACAAAACTAAACGAATTA
AACCGGGTTCTAGGGGGCGGAATTGTCCCTGGGTCCTTAGTGTTAATTGGTGGGGATCCAGGAATTGGAAAGTCAACTTT
ACTGCTACAAGTTTCCGGTCAATTAAGTACGGAGGGGCGTTCTGTTCTCTATGTATCAGGGGAAGAAAGTGCTTCCCAGA
TTAAAATGCGGGCTCAACGCCTTGAAGTAGCAGGAGATAATTTCTACATTTATCCAGAAACAAATATGGATAGTATTCGG
GCAACAATTGATAGTTTAAATCCTGAATATGTAGTGATCGACTCGGTGCAAACAATGCAGGCTACCGATGTTACTTCAGC
AATCGGGAGTGTTTCGCAAATTCGGGCGGTAACGGCACAATTAATGCAGATTGCTAAGGGGAAAAATATTACGGTATTTG
TTGTTGGTCATGTTACCAAGGGTGGGGTCCTTGCCGGGCCTAAAATTTTGGAACATATGGTAGATACAGTACTGTATTTT
GAGGGGGATCTCCATCATACTTACCGGATTTTGCGGGCTGTTAAAAATCGGTTTGGTTCTACCAATGAACTCGGCATTTT
TGAAATGGATAATGATGGTTTAAAAGAGGTTGCTAATCCATCGGAGATCTTCTTGGAGGAACGGTTACAAGATGCCACTG
GGTCAGCGGTGGTGGTTTCCCTTGAAGGTACGCGGCCAATTTTAGTTGAAATTCAAGCTTTGATTACCCCGACCGTTTTT
GGAAATGCGCAACGTACGGCAACGGGCTTAAATCGGAACCGGGTTTCCTTGATTATGGCGGTACTCGAAAAACGAGCAAA
TCTCTTACTTCAAAACCAAGATGCCTACTTAAAGGCAGCAGGAGGTGTCAAACTCGATGAGCCAGCGATTGATTTGGCGA
TTGCCGTTGCGATCGCTTCTTCTTATCGTGATCGCGGAACTCAACCAACGGATGCCTTTGTTGGCGAACTAGGTTTAACT
GGGGAAGTACGTCGGGTTAACCGGATTGAACAACGGGTTGCCGAGGCAGCTAAGCTCGGGTTTAAACGGATTTTTGTCCC
GAAAAATAATTTACAAGGTTGGAAAACCCCGGATAATATTGAGGTAATCGGGGTGACTACCATTAGTGAAACTCTGAAGT
TAGCCTTGGGTAGATAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(364-430)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(70-222)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7L5UJC0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

67.55

98.908

0.668
  radA Streptococcus pneumoniae D39

67.55

98.908

0.668
  radA Streptococcus pneumoniae R6

67.55

98.908

0.668
  radA Streptococcus pneumoniae TIGR4

67.55

98.908

0.668
  radA Streptococcus mitis SK321

67.55

98.908

0.668
  radA Streptococcus mitis NCTC 12261

67.329

98.908

0.666
  radA Bacillus subtilis subsp. subtilis str. 168

63.676

99.782

0.635

Multiple sequence alignment