Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   GTO82_RS01965 Genome accession   NZ_CP047409
Coordinates   425145..426524 (+) Length   459 a.a.
NCBI ID   WP_180873563.1    Uniprot ID   A0A9X7U046
Organism   Lactobacillus johnsonii strain 3DG     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 416739..425145 425145..426524 flank 0
IScluster/Tn 417700..424500 425145..426524 flank 645


Gene organization within MGE regions


Location: 416739..426524
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GTO82_RS01930 (GTO82_01930) - 416739..417617 (+) 879 WP_014567120.1 glycosyltransferase family 2 protein -
  GTO82_RS01935 (GTO82_01935) - 417700..419102 (+) 1403 WP_180873561.1 IS3-like element IS1223 family transposase -
  GTO82_RS01940 (GTO82_01940) - 419157..420170 (-) 1014 WP_014567121.1 sugar transferase -
  GTO82_RS01945 (GTO82_01945) pepC 420454..421803 (-) 1350 WP_086874640.1 aminopeptidase C -
  GTO82_RS01950 (GTO82_01950) - 422001..423035 (+) 1035 WP_180873562.1 LCP family protein -
  GTO82_RS01960 (GTO82_01960) - 424594..425145 (+) 552 WP_014567124.1 hypothetical protein -
  GTO82_RS01965 (GTO82_01965) radA 425145..426524 (+) 1380 WP_180873563.1 DNA repair protein RadA Machinery gene

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 50064.65 Da        Isoelectric Point: 7.6366

>NTDB_id=413328 GTO82_RS01965 WP_180873563.1 425145..426524(+) (radA) [Lactobacillus johnsonii strain 3DG]
MAKVKTRYKCRNCGYISASYLGRCPNCGAWNQFEEETQEIKKVSTKATASRLMTKIGNNDPVKLTEVKAEKEKRIVTPFE
ELNRVLGGGIVPGSLVLIGGDPGIGKSTLMLQITGALAKEHSVLYVSGEESASQIKMRADRLGVSNSGILLYPETNMQNI
RDQIDEIKPDFLVIDSIQTMNEPSLDSMVGSASQVREVTSELMKIAKNEQITTFVIGHVTKEGAIAGPKIMEHMVDTVLY
FEGDGHHSYRILRSVKNRFGAANEIGMFEMKNEGLAEVSNPSAIFLDERLPNSTGSSVVVSLEGTRPLLADIQALVTPTA
FGYAKRTTSGLDFNRVALLLAVLEKRGNLMLQNQDAFLTATGGIKLNEPAIDLAICMAVASSYKNKEISSTDCFVGEVGL
TGEIRRVSQIEARVKEAAKVGFKRIFIPKNNLSQELKNNSEIEVIGVASLPQALKLVFN

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=413328 GTO82_RS01965 WP_180873563.1 425145..426524(+) (radA) [Lactobacillus johnsonii strain 3DG]
ATGGCAAAAGTTAAAACTCGTTATAAGTGTCGTAATTGTGGATATATTTCTGCTTCCTATCTTGGAAGATGTCCCAATTG
TGGTGCTTGGAATCAATTTGAAGAAGAGACACAGGAAATTAAAAAAGTATCAACTAAGGCAACTGCAAGCCGGTTGATGA
CTAAAATTGGAAATAATGATCCAGTGAAGTTAACTGAAGTAAAAGCTGAGAAAGAAAAAAGAATTGTGACTCCTTTTGAA
GAATTAAATCGAGTTTTAGGAGGAGGGATTGTTCCAGGATCTTTAGTTTTAATTGGAGGAGATCCAGGAATTGGAAAATC
AACCTTAATGCTTCAAATTACTGGCGCTTTAGCTAAAGAACATAGCGTCTTATATGTTTCAGGAGAAGAGTCCGCAAGTC
AAATAAAGATGCGGGCAGATCGATTAGGAGTAAGCAATAGCGGAATTTTGCTTTATCCAGAAACAAATATGCAGAATATT
CGTGACCAGATTGATGAAATTAAGCCAGATTTTTTGGTAATTGATTCTATTCAAACAATGAATGAACCTTCACTTGATTC
AATGGTTGGATCTGCTTCTCAAGTACGTGAAGTAACAAGTGAACTAATGAAAATTGCTAAAAATGAACAAATAACTACTT
TTGTTATTGGCCATGTAACTAAAGAAGGAGCAATTGCTGGGCCTAAGATTATGGAACATATGGTTGATACCGTTCTTTAC
TTTGAAGGAGATGGGCACCACTCATATCGAATTTTAAGATCAGTAAAGAATCGTTTTGGTGCAGCTAATGAAATTGGAAT
GTTTGAGATGAAAAATGAAGGGTTAGCAGAAGTAAGTAATCCTTCAGCTATTTTCTTAGACGAACGCTTACCTAATTCGA
CTGGTTCCTCAGTTGTTGTTTCACTTGAAGGAACGCGGCCTCTTCTTGCAGATATCCAAGCTTTAGTAACCCCAACAGCA
TTCGGATATGCAAAAAGAACAACTTCAGGATTAGATTTTAATCGAGTAGCACTTTTATTGGCAGTTTTAGAAAAAAGAGG
CAACTTAATGCTTCAAAACCAAGATGCTTTTCTAACTGCAACTGGTGGGATTAAATTAAATGAACCAGCAATTGATCTTG
CAATTTGCATGGCAGTTGCTTCAAGTTATAAAAATAAAGAAATTTCTTCGACTGATTGCTTTGTAGGCGAAGTTGGCTTA
ACTGGTGAAATTAGAAGAGTTAGTCAAATCGAAGCTAGAGTTAAAGAGGCCGCAAAAGTAGGCTTTAAGAGGATTTTTAT
TCCTAAAAACAACTTAAGCCAGGAACTTAAGAATAACTCTGAGATCGAAGTAATTGGAGTAGCAAGTTTACCACAAGCTT
TAAAACTTGTTTTTAACTAG

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(67-223)

Subunit ChlI of Mg-chelatase (ChlI)

(364-431)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

60.044

99.782

0.599
  radA Streptococcus pneumoniae Rx1

60.044

99.782

0.599
  radA Streptococcus pneumoniae D39

60.044

99.782

0.599
  radA Streptococcus pneumoniae R6

60.044

99.782

0.599
  radA Streptococcus pneumoniae TIGR4

60.044

99.782

0.599
  radA Streptococcus mitis SK321

60.044

99.782

0.599
  radA Bacillus subtilis subsp. subtilis str. 168

56.36

99.346

0.56

Multiple sequence alignment