Detailed information    

insolico Bioinformatically predicted

Overview

Name   pepF   Type   Regulator
Locus tag   GS400_RS05215 Genome accession   NZ_CP047393
Coordinates   995466..997268 (+) Length   600 a.a.
NCBI ID   WP_160099657.1    Uniprot ID   A0A6P1HEQ8
Organism   Pontibacillus sp. HMF3514     
Function   degradation of XIP; competence shut-off (predicted from homology)   
Competence regulation

Genomic Context

Location: 990466..1002268
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GS400_RS05195 (GS400_05195) mecA 990995..991750 (+) 756 WP_370519751.1 adaptor protein MecA -
  GS400_RS05200 (GS400_05200) cls 991902..993413 (+) 1512 WP_160099651.1 cardiolipin synthase -
  GS400_RS05205 (GS400_05205) - 993707..993934 (-) 228 WP_236561154.1 hypothetical protein -
  GS400_RS05210 (GS400_05210) - 994110..995273 (+) 1164 WP_160099655.1 competence protein CoiA -
  GS400_RS05215 (GS400_05215) pepF 995466..997268 (+) 1803 WP_160099657.1 oligoendopeptidase F Regulator
  GS400_RS05220 (GS400_05220) - 997603..998493 (-) 891 WP_370519752.1 ClpXP adapter SpxH family protein -
  GS400_RS05225 (GS400_05225) - 998496..998894 (-) 399 WP_160099659.1 globin -
  GS400_RS05230 (GS400_05230) - 999047..999628 (-) 582 WP_160099661.1 CYTH domain-containing protein -
  GS400_RS05235 (GS400_05235) - 999961..1000584 (+) 624 WP_160104519.1 GTP pyrophosphokinase family protein -
  GS400_RS05240 (GS400_05240) - 1000620..1001420 (+) 801 WP_160099663.1 NAD kinase -

Sequence

Protein


Download         Length: 600 a.a.        Molecular weight: 69392.52 Da        Isoelectric Point: 4.7123

>NTDB_id=413208 GS400_RS05215 WP_160099657.1 995466..997268(+) (pepF) [Pontibacillus sp. HMF3514]
MAKELPKREEIPEERTWRLEDIFESDEAWNKEYDAVKELVPQITEYQGKLHESADTLYNLLKLQDEVASRFGKLFTYAHM
RYDQDTTNSHYQEMNTRAENLLTQVGSAMSYIDPEILAMEEGTVEKFLQEKEELKLYEHALNEITRGRPHTLSEKEEALL
AQAGEVMGNPAQTFGMLNNADLTFPTIKDEEGNEVDLTHGRYIRFLQSKDRSVRKAAFEAMYDTFGKFKNTFSSTLSGAV
KKHNFSANIRNYDSARHSALDKNNIPEKVYDNLVEAVNDRLPALHRYVELRKEVLGLDELHMYDLYADLVQDVDMDIPYE
EAKELVLKGLEPLGEEYTNILKEGYKNRWIDVDENKGKRSGAYSSGSYGTNPYILMNWQNNLNNLFTLAHELGHSLHSYY
TTNNQPYRYGNYSIFVAEVASTCNEALLNDYLLKETTDKKRKLYLLNNFLEGFRGTVYRQTMFAEFEHDIHKREQNGEAL
TADKLTELYHDLNEKYFGKNIVVDDEIGLEWARIPHFYYNYYVYQYATGYSAATALADQILKEGDSAVERYKGFLKAGSS
DYPIEVLKHAGVDMTSKDPIEAALDVFEEKLAEFEKLVKE

Nucleotide


Download         Length: 1803 bp        

>NTDB_id=413208 GS400_RS05215 WP_160099657.1 995466..997268(+) (pepF) [Pontibacillus sp. HMF3514]
TTGGCTAAAGAATTACCTAAACGCGAAGAGATACCAGAGGAACGCACATGGAGATTAGAAGATATCTTTGAGTCTGATGA
GGCTTGGAATAAAGAGTATGACGCAGTAAAAGAACTAGTTCCACAAATTACAGAGTATCAGGGTAAATTACATGAATCAG
CAGATACACTATATAACCTATTAAAACTCCAAGATGAGGTAGCGTCTCGTTTCGGCAAATTGTTTACATACGCACACATG
CGCTACGACCAAGATACGACCAATTCCCATTACCAAGAAATGAACACTCGTGCTGAGAACCTACTAACTCAAGTAGGAAG
TGCCATGAGTTACATTGATCCTGAAATTCTTGCAATGGAAGAGGGAACGGTTGAAAAGTTTTTACAAGAAAAAGAAGAGC
TGAAGTTGTACGAGCACGCATTAAATGAAATTACACGAGGCCGTCCTCACACGTTAAGTGAAAAAGAGGAAGCATTACTA
GCACAAGCTGGTGAAGTAATGGGGAATCCAGCTCAAACATTCGGTATGTTGAATAACGCTGACTTAACATTCCCAACAAT
TAAAGATGAAGAGGGAAATGAGGTAGACCTTACACATGGTCGCTACATTCGCTTCTTACAGTCGAAAGATCGTTCCGTAC
GTAAAGCAGCTTTCGAAGCGATGTATGACACGTTTGGTAAGTTCAAGAATACGTTTTCTTCAACATTGAGTGGGGCAGTA
AAAAAGCATAACTTCTCAGCAAATATTCGTAACTATGATTCTGCGCGCCATTCTGCGTTAGATAAAAACAATATCCCTGA
AAAAGTGTATGATAACTTAGTTGAAGCTGTTAATGACCGCTTACCAGCTCTTCATCGCTACGTAGAATTACGTAAAGAAG
TGCTAGGTCTTGATGAGCTTCATATGTATGACTTATATGCGGATCTTGTGCAGGATGTTGATATGGACATTCCTTATGAG
GAAGCAAAGGAGCTTGTGTTAAAAGGTCTTGAGCCATTAGGGGAAGAATACACGAACATTCTTAAAGAAGGTTATAAAAA
TCGCTGGATCGACGTGGATGAAAACAAAGGAAAGCGTAGTGGAGCGTATTCTTCAGGTTCTTACGGTACAAACCCTTACA
TCCTGATGAATTGGCAAAATAACCTGAACAACTTATTTACATTAGCTCACGAGCTAGGTCATTCCTTACACAGCTACTAT
ACAACGAATAATCAGCCTTATCGTTATGGAAACTACTCGATTTTCGTAGCAGAGGTAGCCTCAACGTGTAATGAAGCATT
GTTAAATGACTATCTGCTAAAAGAAACAACAGATAAAAAGCGTAAACTTTATCTTCTTAATAACTTCTTAGAAGGCTTCC
GTGGCACGGTGTACCGTCAAACCATGTTCGCTGAGTTTGAGCATGATATTCATAAGCGTGAACAAAACGGGGAAGCCCTA
ACAGCTGATAAACTGACTGAACTCTATCATGATTTAAATGAAAAGTACTTCGGTAAAAATATCGTAGTGGATGACGAAAT
TGGCCTGGAATGGGCTCGTATTCCTCACTTCTATTACAACTACTATGTTTATCAGTATGCGACAGGATACTCCGCAGCAA
CAGCATTAGCGGACCAAATCCTTAAAGAAGGTGACTCAGCAGTTGAGCGTTACAAAGGCTTCTTAAAAGCGGGAAGTAGC
GATTACCCAATTGAAGTGTTGAAGCATGCTGGAGTAGATATGACATCGAAAGATCCAATCGAAGCAGCTCTTGATGTATT
CGAAGAAAAGCTTGCTGAATTTGAGAAATTAGTAAAAGAATAA

Domains

Predicted by InterproScan.

Oligopeptidase F (Peptidase_M3_N)

(115-184)

Peptidase family M3 (Peptidase_M3)

(206-584)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6P1HEQ8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pepF Streptococcus salivarius strain HSISS4

48.735

98.833

0.482

Multiple sequence alignment