Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   GS400_RS00655 Genome accession   NZ_CP047393
Coordinates   123025..124404 (+) Length   459 a.a.
NCBI ID   WP_027446565.1    Uniprot ID   A0A0A5G8C0
Organism   Pontibacillus sp. HMF3514     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 118025..129404
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GS400_RS00635 (GS400_00635) - 118238..118702 (+) 465 WP_160098216.1 CtsR family transcriptional regulator -
  GS400_RS00640 (GS400_00640) - 118720..119274 (+) 555 WP_160098218.1 UvrB/UvrC motif-containing protein -
  GS400_RS00645 (GS400_00645) - 119274..120338 (+) 1065 WP_160098220.1 protein arginine kinase -
  GS400_RS00650 (GS400_00650) clpC 120360..122789 (+) 2430 WP_160098222.1 ATP-dependent protease ATP-binding subunit ClpC -
  GS400_RS00655 (GS400_00655) radA 123025..124404 (+) 1380 WP_027446565.1 DNA repair protein RadA Machinery gene
  GS400_RS00660 (GS400_00660) disA 124405..125478 (+) 1074 WP_160098223.1 DNA integrity scanning diadenylate cyclase DisA -
  GS400_RS00665 (GS400_00665) - 125659..126765 (+) 1107 WP_160098225.1 PIN/TRAM domain-containing protein -
  GS400_RS00670 (GS400_00670) - 126871..128055 (+) 1185 WP_160098227.1 bifunctional 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase/2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 50109.51 Da        Isoelectric Point: 7.7626

>NTDB_id=413200 GS400_RS00655 WP_027446565.1 123025..124404(+) (radA) [Pontibacillus sp. HMF3514]
MAKQKSKFVCQECGYETPKWMGKCPGCHKWNTLVEERVASNTSGRHTFVTGDTSGKVKPEKITEIASQKEPRIETDMPEV
NRVLGGGIVPGSLVLIGGDPGIGKSTILLQVSSQLAHKKNKVLYISGEESPKQTKLRADRLGVLSDHLYVLSETNMADVS
QNIDQIQPSFVVIDSIQTIFKEEVASAPGSVSQVRECTSELMRIAKQKGIPIFIVGHVTKEGSIAGPRLLEHMVDAVLYF
EGERHHTFRILRSVKNRFGSTHEMGIFEMKEEGLREVENPSEIFLEERSQGAAGSTVVASMEGTRPILVEIQSLISPTSY
GNPRRMATGVDSNRVPLLMAVLEKRVGLLLQNQDAYVKVAGGVKLDEPAIDLAVAVSIASSFQDRASKADDVLIGEVGLT
GEVRRVARIEQRVQEAAKLGFKRAIVPKKNLSGWTFPDNIEIIGVNSVQEALKQTLGGS

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=413200 GS400_RS00655 WP_027446565.1 123025..124404(+) (radA) [Pontibacillus sp. HMF3514]
ATGGCCAAGCAAAAATCGAAATTTGTATGTCAGGAATGTGGTTATGAGACGCCAAAATGGATGGGAAAGTGTCCAGGGTG
TCATAAATGGAACACTCTTGTAGAAGAGAGAGTAGCATCGAATACATCAGGTCGGCATACATTTGTAACAGGGGATACTT
CTGGTAAAGTAAAGCCGGAAAAGATAACAGAAATTGCATCCCAAAAGGAACCACGAATTGAAACAGATATGCCAGAGGTG
AACCGCGTACTTGGAGGAGGGATCGTACCAGGATCACTCGTCCTCATTGGTGGTGACCCCGGAATTGGTAAGTCTACCAT
TCTGCTTCAAGTTTCATCTCAATTGGCTCATAAAAAGAATAAAGTACTTTATATATCCGGTGAGGAATCTCCTAAGCAAA
CAAAGCTGCGAGCAGATCGTTTAGGGGTTTTATCAGATCATCTATATGTCTTGTCAGAGACCAATATGGCGGATGTTTCT
CAAAATATTGATCAAATCCAACCATCATTTGTTGTAATTGACTCTATTCAAACCATATTCAAAGAAGAAGTAGCGTCTGC
TCCTGGAAGTGTCTCTCAGGTGAGAGAATGTACAAGTGAATTAATGCGAATAGCTAAACAAAAAGGAATTCCGATATTCA
TAGTTGGACATGTCACCAAAGAAGGGTCTATTGCAGGTCCACGCTTACTCGAGCATATGGTTGATGCTGTTTTATATTTT
GAAGGAGAACGTCACCATACCTTCCGAATTTTACGAAGTGTTAAAAACCGTTTTGGTAGTACACACGAAATGGGTATCTT
TGAAATGAAAGAAGAAGGTTTGCGAGAAGTAGAAAATCCTTCTGAAATCTTTCTAGAAGAACGTTCGCAAGGTGCAGCTG
GATCCACAGTTGTTGCGTCCATGGAAGGAACACGCCCGATTCTTGTTGAAATCCAATCTCTTATTTCTCCAACAAGCTAC
GGAAACCCAAGAAGGATGGCTACTGGAGTAGACAGTAATCGTGTACCGCTATTAATGGCAGTACTAGAAAAACGTGTTGG
GTTGCTTCTGCAAAATCAAGATGCTTATGTAAAGGTAGCAGGTGGGGTTAAGCTAGATGAACCTGCAATTGATTTGGCAG
TAGCCGTAAGTATTGCCTCGAGCTTTCAAGACCGTGCATCTAAGGCAGATGATGTCTTAATTGGAGAGGTTGGATTAACA
GGTGAAGTAAGAAGGGTTGCTCGAATTGAACAGCGTGTCCAAGAAGCTGCAAAGTTAGGGTTTAAGCGTGCAATTGTACC
TAAGAAGAACCTTAGTGGATGGACATTCCCTGATAACATAGAGATTATCGGCGTGAATTCTGTTCAGGAGGCCTTGAAGC
AGACGTTGGGAGGATCATAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(65-222)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(363-430)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0A5G8C0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

73.144

99.782

0.73
  radA Streptococcus pneumoniae Rx1

61.488

99.564

0.612
  radA Streptococcus pneumoniae D39

61.488

99.564

0.612
  radA Streptococcus pneumoniae R6

61.488

99.564

0.612
  radA Streptococcus pneumoniae TIGR4

61.488

99.564

0.612
  radA Streptococcus mitis NCTC 12261

61.488

99.564

0.612
  radA Streptococcus mitis SK321

61.488

99.564

0.612

Multiple sequence alignment