Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   PJ20_RS02665 Genome accession   NZ_CP047386
Coordinates   597653..599140 (-) Length   495 a.a.
NCBI ID   WP_039373743.1    Uniprot ID   A0A5E4TYH5
Organism   Pandoraea fibrosis strain 7641     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 592653..604140
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PJ20_RS02635 (PJ20_002635) mutY 592725..593882 (+) 1158 WP_039373735.1 A/G-specific adenine glycosylase -
  PJ20_RS02640 (PJ20_002640) rapZ 593884..594765 (-) 882 WP_039373736.1 RNase adapter RapZ -
  PJ20_RS02645 (PJ20_002645) - 594937..595263 (-) 327 WP_039373738.1 PsiF family protein -
  PJ20_RS02650 (PJ20_002650) hprK 595422..596387 (-) 966 WP_010804807.1 HPr(Ser) kinase/phosphatase -
  PJ20_RS02655 (PJ20_002655) ptsN 596465..596920 (-) 456 WP_039373740.1 PTS IIA-like nitrogen regulatory protein PtsN -
  PJ20_RS02660 (PJ20_002660) hpf 597157..597510 (-) 354 WP_039373742.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  PJ20_RS02665 (PJ20_002665) rpoN/rpoN1 597653..599140 (-) 1488 WP_039373743.1 RNA polymerase factor sigma-54 Machinery gene
  PJ20_RS02670 (PJ20_002670) lptB 599235..600005 (-) 771 WP_039373831.1 LPS export ABC transporter ATP-binding protein -
  PJ20_RS02675 (PJ20_002675) lptA 600031..600732 (-) 702 WP_039373744.1 lipopolysaccharide transport periplasmic protein LptA -
  PJ20_RS02680 (PJ20_002680) lptC 600729..601388 (-) 660 WP_039373745.1 LPS export ABC transporter periplasmic protein LptC -
  PJ20_RS02685 (PJ20_002685) - 601412..601984 (-) 573 WP_080759364.1 KdsC family phosphatase -
  PJ20_RS02690 (PJ20_002690) - 601996..602976 (-) 981 WP_039373748.1 KpsF/GutQ family sugar-phosphate isomerase -

Sequence

Protein


Download         Length: 495 a.a.        Molecular weight: 55182.05 Da        Isoelectric Point: 5.2778

>NTDB_id=413154 PJ20_RS02665 WP_039373743.1 597653..599140(-) (rpoN/rpoN1) [Pandoraea fibrosis strain 7641]
MKPTLQLRTSQHLTLTPQLQQSIRLLQLSTLELQQEVEHALTQNPMLEREDDWLAGSVRVGTDGSLRNERSTSASHTNDG
SEPANGRDEARELDGEARFDDNAQDNGSDWSLNDFARSGNASDDDDNARPQLHVDHPNLREHLLAQLRLTKAAPRDRALV
AFLIESLDEDGYLGTTLDDIEAEMPEELALETEEINAALALLQSFDPAGVGARGPAECLRLQLQRLPASSVRTLSLRIVS
DHLELLAARDYTRLRRLLGVSEDSLRAAHQLIRSLDPFPGAAYGSPQADYVIPDVLVRRQGSGWTAELNPDVMPRLRINE
MYARILRNNRNDPGTGNLQQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFTHGEIGMRPLVLREIADTLGLHESTI
SRVTTSKYMLTPFGTFELKYFFGSHVATEAGGAASSTAIRALIKQLIGAEDPQSPLSDSRIAELLAEQGFVVARRTVAKY
REAMRIPAVNLRKSL

Nucleotide


Download         Length: 1488 bp        

>NTDB_id=413154 PJ20_RS02665 WP_039373743.1 597653..599140(-) (rpoN/rpoN1) [Pandoraea fibrosis strain 7641]
ATGAAACCGACTCTTCAACTCCGCACCTCGCAGCACCTGACCCTCACGCCGCAGTTGCAGCAATCGATCCGGTTGTTGCA
ACTGTCTACGCTCGAACTTCAGCAGGAAGTCGAGCACGCCCTCACCCAGAACCCGATGCTCGAACGCGAGGACGATTGGC
TCGCGGGCTCGGTACGTGTGGGCACGGACGGGTCGTTGCGCAACGAGCGGAGCACGTCGGCCAGTCATACGAACGACGGC
TCCGAACCCGCCAACGGCCGCGATGAAGCCCGCGAACTCGACGGCGAAGCCCGCTTCGACGACAACGCGCAAGACAACGG
CAGCGACTGGAGCCTGAACGACTTCGCTCGCTCGGGCAACGCCTCGGACGACGACGACAACGCGCGTCCGCAATTGCACG
TCGACCATCCCAATCTTCGCGAACACCTGCTGGCGCAGTTGCGCCTGACCAAAGCCGCGCCGCGCGACCGTGCGCTGGTG
GCATTCCTGATCGAATCGCTCGACGAAGACGGCTATCTCGGCACCACGCTCGACGACATCGAGGCAGAAATGCCCGAAGA
GCTGGCGCTGGAAACGGAAGAGATCAACGCCGCGCTTGCCCTGCTGCAAAGCTTCGACCCCGCGGGCGTGGGGGCCCGCG
GTCCGGCGGAATGCCTGCGCCTGCAACTCCAGCGCCTGCCCGCGAGCAGCGTTCGCACGCTCTCGCTGCGTATCGTCAGC
GATCACCTGGAACTGCTCGCCGCCCGCGATTACACTCGCCTGCGCCGCCTGCTGGGCGTGAGCGAAGACAGCTTGCGTGC
CGCCCATCAACTGATTCGCTCGCTCGACCCGTTCCCCGGCGCCGCCTATGGTTCGCCTCAGGCCGATTACGTCATCCCCG
ATGTGCTCGTGCGCAGACAGGGCAGCGGCTGGACCGCAGAGCTGAATCCCGACGTCATGCCGCGCCTGCGCATCAACGAG
ATGTACGCGCGCATTCTGCGCAATAACCGCAACGATCCGGGCACCGGCAACTTGCAGCAGCAGCTTCAGGAAGCGCGCTG
GCTGATCAAAAACATTCAGCAGCGCTTCGATACGATCCTGCGCGTGGCGCAGGCGATTGTCGAGCGTCAAAAGAACTTCT
TCACGCACGGCGAAATCGGCATGCGGCCCTTGGTTTTACGCGAGATTGCTGATACGCTGGGTTTACACGAATCCACGATT
TCACGCGTGACTACCAGTAAGTACATGCTCACTCCATTCGGAACCTTTGAGCTGAAGTACTTTTTTGGTTCACATGTGGC
AACGGAAGCCGGGGGCGCAGCGTCATCGACCGCCATCCGCGCACTCATCAAGCAACTCATAGGAGCAGAAGACCCGCAGA
GCCCCCTTTCCGACAGCCGTATCGCGGAACTGCTGGCAGAGCAAGGGTTCGTGGTAGCGCGGCGCACCGTCGCGAAATAC
CGCGAGGCCATGCGAATCCCCGCAGTGAACTTGCGCAAATCTTTGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(133-322)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(338-493)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5E4TYH5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.463

100

0.693

Multiple sequence alignment