Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   GO994_RS01945 Genome accession   NZ_CP047376
Coordinates   376890..378329 (+) Length   479 a.a.
NCBI ID   WP_159364285.1    Uniprot ID   -
Organism   Aeromonas salmonicida subsp. salmonicida strain J410     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 371890..383329
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GO994_RS01915 (GO994_01915) mlaF 372467..373267 (-) 801 WP_011898278.1 phospholipid ABC transporter ATP-binding protein MlaF -
  GO994_RS01920 (GO994_01920) - 373475..374470 (+) 996 WP_011898279.1 KpsF/GutQ family sugar-phosphate isomerase -
  GO994_RS01925 (GO994_01925) kdsC 374470..375024 (+) 555 WP_042468044.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  GO994_RS01930 (GO994_01930) lptC 375021..375581 (+) 561 WP_042468042.1 LPS export ABC transporter periplasmic protein LptC -
  GO994_RS01935 (GO994_01935) lptA 375568..376095 (+) 528 WP_005314414.1 lipopolysaccharide transport periplasmic protein LptA -
  GO994_RS01940 (GO994_01940) lptB 376099..376824 (+) 726 WP_005314412.1 LPS export ABC transporter ATP-binding protein -
  GO994_RS01945 (GO994_01945) rpoN/rpoN1 376890..378329 (+) 1440 WP_159364285.1 RNA polymerase factor sigma-54 Machinery gene
  GO994_RS01950 (GO994_01950) hpf 378351..378638 (+) 288 WP_005305957.1 ribosome hibernation promoting factor -
  GO994_RS01955 (GO994_01955) ptsN 378641..379087 (+) 447 WP_005314406.1 PTS IIA-like nitrogen regulatory protein PtsN -
  GO994_RS01960 (GO994_01960) rapZ 379130..379996 (+) 867 WP_005314405.1 RNase adapter RapZ -
  GO994_RS01965 (GO994_01965) npr 380017..380289 (+) 273 WP_005314403.1 PTS phosphocarrier protein NPr -
  GO994_RS01970 (GO994_01970) - 380308..382212 (-) 1905 WP_159364286.1 transcription antiterminator -
  GO994_RS01975 (GO994_01975) dagF 382316..383056 (-) 741 WP_005314402.1 2-dehydro-3-deoxy-phosphogluconate aldolase -

Sequence

Protein


Download         Length: 479 a.a.        Molecular weight: 53992.95 Da        Isoelectric Point: 4.3718

>NTDB_id=413091 GO994_RS01945 WP_159364285.1 376890..378329(+) (rpoN/rpoN1) [Aeromonas salmonicida subsp. salmonicida strain J410]
MKPTLQLRLGQSLTMTPQLQQAIRLLQLSTLELQQEIQQALDNNPLLEQEENDVQETSPEEPVDDSQLDSSDAMAQDQMP
DELPVDTTWEDVYSAGTAQSAGPIHDGEDSVYQGETTENLQDYLLWQMRLTPFSDLDAAIALAIIDAIDDSGYLTVSLED
IQAAVSSEDVEVELDEVEAVLKRVQHFDPVGITARSVQECLLIQLGQYAPELPWLNEAREVIKEHMDLLGNRDYRTLARK
TKLKESDLKEVLDLIQQLEPRPGNGIIQSDSQYVIPDVTVVKKGNKWVVELNPDSAPRIRVNETYAAMARQARTSQDTQF
IRSHLQEAKWFIKSLESRNDTLLKVATCIVEQQQGFFEYGEEAMKPMVLNDIAEAVEMHESTISRVTTQKYMHTPRGIFE
LKFFFSSHVNTEGGRECSSTAIRALIKKLVTAENPAKPLSDSKIADLLAEQGIMVARRTIAKYRESLLIPPSNQRKRLV

Nucleotide


Download         Length: 1440 bp        

>NTDB_id=413091 GO994_RS01945 WP_159364285.1 376890..378329(+) (rpoN/rpoN1) [Aeromonas salmonicida subsp. salmonicida strain J410]
ATGAAGCCGACATTACAGTTGCGTCTCGGTCAGTCCCTGACCATGACGCCGCAATTACAACAAGCGATTCGTCTGCTCCA
GCTCTCCACCCTGGAACTCCAGCAGGAAATTCAGCAAGCGCTCGATAACAATCCTTTGCTTGAGCAAGAGGAGAATGACG
TTCAGGAGACCAGCCCGGAAGAGCCCGTCGACGACAGTCAGCTCGACTCCAGCGATGCCATGGCCCAGGATCAGATGCCG
GACGAACTGCCGGTAGATACCACCTGGGAAGATGTCTATTCAGCCGGCACAGCCCAGAGCGCAGGCCCCATCCACGATGG
GGAGGACAGCGTCTATCAAGGGGAAACCACCGAGAACCTGCAGGACTATCTGCTGTGGCAGATGCGCCTCACCCCGTTCA
GCGATCTGGATGCCGCCATTGCGCTGGCCATCATAGATGCCATCGATGACTCCGGTTATCTGACCGTCTCTCTCGAAGAT
ATACAGGCGGCGGTGAGCAGCGAGGATGTCGAGGTGGAACTCGACGAGGTCGAGGCAGTGCTCAAGCGGGTGCAGCATTT
CGATCCGGTCGGCATCACCGCGCGCTCGGTGCAGGAGTGTCTGCTGATCCAGCTTGGCCAGTACGCTCCTGAACTGCCCT
GGCTCAATGAGGCCAGGGAGGTAATCAAGGAGCACATGGACTTGCTCGGCAACCGTGATTACCGCACCCTGGCCCGCAAG
ACCAAGCTCAAGGAGAGTGACCTCAAGGAGGTGCTGGACCTTATCCAGCAACTGGAGCCCAGGCCGGGTAACGGCATCAT
ACAGAGTGACTCCCAGTACGTGATCCCGGATGTCACCGTCGTCAAGAAGGGCAACAAATGGGTCGTTGAGCTCAACCCTG
ATTCTGCACCGCGCATCCGGGTCAACGAGACCTATGCCGCCATGGCCCGTCAGGCGCGTACCAGCCAGGATACCCAGTTC
ATTCGCTCCCACTTGCAAGAAGCCAAGTGGTTCATCAAGAGTCTGGAGAGTCGCAACGACACCCTGCTCAAGGTGGCGAC
CTGTATTGTCGAGCAACAACAGGGCTTTTTCGAGTATGGTGAAGAAGCCATGAAGCCCATGGTGCTCAACGATATCGCCG
AGGCGGTGGAGATGCATGAGTCGACCATTTCCAGGGTAACGACCCAGAAATACATGCATACCCCCCGCGGCATCTTCGAA
CTGAAGTTTTTTTTCTCCAGTCATGTGAACACAGAAGGAGGAAGAGAGTGCTCATCGACCGCAATTCGCGCGCTGATCAA
GAAGCTGGTGACGGCGGAGAATCCCGCCAAGCCGCTAAGTGATAGCAAGATCGCGGATTTGCTGGCCGAACAGGGTATTA
TGGTGGCGAGACGCACGATCGCAAAGTACCGTGAATCCTTGTTGATCCCGCCATCCAATCAGCGCAAACGCCTGGTTTAG

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(319-477)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-305)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.58

100

0.484

Multiple sequence alignment