Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   GO992_RS15215 Genome accession   NZ_CP047374
Coordinates   3214976..3216415 (+) Length   479 a.a.
NCBI ID   WP_159364285.1    Uniprot ID   -
Organism   Aeromonas salmonicida subsp. salmonicida strain J409     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3209976..3221415
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GO992_RS15185 (GO992_15185) mlaF 3210553..3211353 (-) 801 WP_011898278.1 phospholipid ABC transporter ATP-binding protein MlaF -
  GO992_RS15190 (GO992_15190) - 3211561..3212556 (+) 996 WP_011898279.1 KpsF/GutQ family sugar-phosphate isomerase -
  GO992_RS15195 (GO992_15195) kdsC 3212556..3213110 (+) 555 WP_042468044.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  GO992_RS15200 (GO992_15200) lptC 3213107..3213667 (+) 561 WP_042468042.1 LPS export ABC transporter periplasmic protein LptC -
  GO992_RS15205 (GO992_15205) lptA 3213654..3214181 (+) 528 WP_005314414.1 lipopolysaccharide transport periplasmic protein LptA -
  GO992_RS15210 (GO992_15210) lptB 3214185..3214910 (+) 726 WP_005314412.1 LPS export ABC transporter ATP-binding protein -
  GO992_RS15215 (GO992_15215) rpoN/rpoN1 3214976..3216415 (+) 1440 WP_159364285.1 RNA polymerase factor sigma-54 Machinery gene
  GO992_RS15220 (GO992_15220) hpf 3216437..3216724 (+) 288 WP_005305957.1 ribosome hibernation promoting factor -
  GO992_RS15225 (GO992_15225) ptsN 3216727..3217173 (+) 447 WP_005314406.1 PTS IIA-like nitrogen regulatory protein PtsN -
  GO992_RS15230 (GO992_15230) rapZ 3217216..3218082 (+) 867 WP_005314405.1 RNase adapter RapZ -
  GO992_RS15235 (GO992_15235) npr 3218103..3218375 (+) 273 WP_005314403.1 PTS phosphocarrier protein NPr -
  GO992_RS15240 (GO992_15240) - 3218394..3220298 (-) 1905 WP_159364286.1 transcription antiterminator -
  GO992_RS15245 (GO992_15245) dagF 3220402..3221142 (-) 741 WP_005314402.1 2-dehydro-3-deoxy-phosphogluconate aldolase -

Sequence

Protein


Download         Length: 479 a.a.        Molecular weight: 53992.95 Da        Isoelectric Point: 4.3718

>NTDB_id=413081 GO992_RS15215 WP_159364285.1 3214976..3216415(+) (rpoN/rpoN1) [Aeromonas salmonicida subsp. salmonicida strain J409]
MKPTLQLRLGQSLTMTPQLQQAIRLLQLSTLELQQEIQQALDNNPLLEQEENDVQETSPEEPVDDSQLDSSDAMAQDQMP
DELPVDTTWEDVYSAGTAQSAGPIHDGEDSVYQGETTENLQDYLLWQMRLTPFSDLDAAIALAIIDAIDDSGYLTVSLED
IQAAVSSEDVEVELDEVEAVLKRVQHFDPVGITARSVQECLLIQLGQYAPELPWLNEAREVIKEHMDLLGNRDYRTLARK
TKLKESDLKEVLDLIQQLEPRPGNGIIQSDSQYVIPDVTVVKKGNKWVVELNPDSAPRIRVNETYAAMARQARTSQDTQF
IRSHLQEAKWFIKSLESRNDTLLKVATCIVEQQQGFFEYGEEAMKPMVLNDIAEAVEMHESTISRVTTQKYMHTPRGIFE
LKFFFSSHVNTEGGRECSSTAIRALIKKLVTAENPAKPLSDSKIADLLAEQGIMVARRTIAKYRESLLIPPSNQRKRLV

Nucleotide


Download         Length: 1440 bp        

>NTDB_id=413081 GO992_RS15215 WP_159364285.1 3214976..3216415(+) (rpoN/rpoN1) [Aeromonas salmonicida subsp. salmonicida strain J409]
ATGAAGCCGACATTACAGTTGCGTCTCGGTCAGTCCCTGACCATGACGCCGCAATTACAACAAGCGATTCGTCTGCTCCA
GCTCTCCACCCTGGAACTCCAGCAGGAAATTCAGCAAGCGCTCGATAACAATCCTTTGCTTGAGCAAGAGGAGAATGACG
TTCAGGAGACCAGCCCGGAAGAGCCCGTCGACGACAGTCAGCTCGACTCCAGCGATGCCATGGCCCAGGATCAGATGCCG
GACGAACTGCCGGTAGATACCACCTGGGAAGATGTCTATTCAGCCGGCACAGCCCAGAGCGCAGGCCCCATCCACGATGG
GGAGGACAGCGTCTATCAAGGGGAAACCACCGAGAACCTGCAGGACTATCTGCTGTGGCAGATGCGCCTCACCCCGTTCA
GCGATCTGGATGCCGCCATTGCGCTGGCCATCATAGATGCCATCGATGACTCCGGTTATCTGACCGTCTCTCTCGAAGAT
ATACAGGCGGCGGTGAGCAGCGAGGATGTCGAGGTGGAACTCGACGAGGTCGAGGCAGTGCTCAAGCGGGTGCAGCATTT
CGATCCGGTCGGCATCACCGCGCGCTCGGTGCAGGAGTGTCTGCTGATCCAGCTTGGCCAGTACGCTCCTGAACTGCCCT
GGCTCAATGAGGCCAGGGAGGTAATCAAGGAGCACATGGACTTGCTCGGCAACCGTGATTACCGCACCCTGGCCCGCAAG
ACCAAGCTCAAGGAGAGTGACCTCAAGGAGGTGCTGGACCTTATCCAGCAACTGGAGCCCAGGCCGGGTAACGGCATCAT
ACAGAGTGACTCCCAGTACGTGATCCCGGATGTCACCGTCGTCAAGAAGGGCAACAAATGGGTCGTTGAGCTCAACCCTG
ATTCTGCACCGCGCATCCGGGTCAACGAGACCTATGCCGCCATGGCCCGTCAGGCGCGTACCAGCCAGGATACCCAGTTC
ATTCGCTCCCACTTGCAAGAAGCCAAGTGGTTCATCAAGAGTCTGGAGAGTCGCAACGACACCCTGCTCAAGGTGGCGAC
CTGTATTGTCGAGCAACAACAGGGCTTTTTCGAGTATGGTGAAGAAGCCATGAAGCCCATGGTGCTCAACGATATCGCCG
AGGCGGTGGAGATGCATGAGTCGACCATTTCCAGGGTAACGACCCAGAAATACATGCATACCCCCCGCGGCATCTTCGAA
CTGAAGTTTTTTTTCTCCAGTCATGTGAACACAGAAGGAGGAAGAGAGTGCTCATCGACCGCAATTCGCGCGCTGATCAA
GAAGCTGGTGACGGCGGAGAATCCCGCCAAGCCGCTAAGTGATAGCAAGATCGCGGATTTGCTGGCCGAACAGGGTATTA
TGGTGGCGAGACGCACGATCGCAAAGTACCGTGAATCCTTGTTGATCCCGCCATCCAATCAGCGCAAACGCCTGGTTTAG

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(319-477)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-305)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.58

100

0.484

Multiple sequence alignment