Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   GSN03_RS20360 Genome accession   NZ_CP047366
Coordinates   3967086..3968129 (-) Length   347 a.a.
NCBI ID   WP_212088009.1    Uniprot ID   -
Organism   Bacillus nitratireducens strain BM02     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3962086..3973129
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GSN03_RS20315 (GSN03_20150) - 3962486..3962686 (-) 201 WP_000106081.1 YqzE family protein -
  GSN03_RS20320 (GSN03_20155) aroK 3962725..3963222 (-) 498 WP_000836667.1 shikimate kinase AroK -
  GSN03_RS20325 (GSN03_20160) - 3963374..3964024 (-) 651 WP_002131210.1 2OG-Fe(II) oxygenase -
  GSN03_RS20330 (GSN03_20165) comGG 3964198..3964568 (-) 371 Protein_4006 competence type IV pilus minor pilin ComGG -
  GSN03_RS20335 (GSN03_20170) comGF 3964565..3965026 (-) 462 WP_001189035.1 competence type IV pilus minor pilin ComGF -
  GSN03_RS20340 (GSN03_20175) - 3965010..3965306 (-) 297 WP_000230010.1 hypothetical protein -
  GSN03_RS20345 (GSN03_20180) comGD 3965299..3965754 (-) 456 WP_212088008.1 competence type IV pilus minor pilin ComGD -
  GSN03_RS20350 (GSN03_20185) comGC 3965751..3966050 (-) 300 WP_002145747.1 competence type IV pilus major pilin ComGC -
  GSN03_RS20355 (GSN03_20190) comGB 3966062..3967099 (-) 1038 Protein_4011 competence type IV pilus assembly protein ComGB -
  GSN03_RS20360 (GSN03_20195) comGA 3967086..3968129 (-) 1044 WP_212088009.1 competence type IV pilus ATPase ComGA Machinery gene
  GSN03_RS20365 (GSN03_20200) - 3968335..3969030 (+) 696 WP_212088010.1 helix-turn-helix transcriptional regulator -
  GSN03_RS20370 (GSN03_20205) - 3969161..3969403 (+) 243 WP_000440717.1 DUF2626 domain-containing protein -
  GSN03_RS20375 (GSN03_20210) - 3969511..3970905 (+) 1395 WP_001094337.1 L-cystine transporter -
  GSN03_RS20380 (GSN03_20215) - 3971114..3971500 (-) 387 WP_245002997.1 hypothetical protein -
  GSN03_RS20385 (GSN03_20220) - 3971588..3971803 (-) 216 WP_044737804.1 DUF3912 family protein -
  GSN03_RS20390 (GSN03_20225) - 3972056..3972367 (+) 312 WP_212088012.1 hypothetical protein -
  GSN03_RS20395 (GSN03_20230) - 3972402..3972890 (-) 489 WP_000764257.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39209.55 Da        Isoelectric Point: 8.6514

>NTDB_id=413047 GSN03_RS20360 WP_212088009.1 3967086..3968129(-) (comGA) [Bacillus nitratireducens strain BM02]
MNSVELFANMIMKEACGVQASDLHIVPRQKDMAIQLRIGKDLIMKRCIEKEFGEKLVSHFKFLASMDIGERRKPQNGSLY
LQIDGQEVYLRLSTLPTVYQESLVIRLHLQASSQPLSHLSLFPSSAEKLLSFLKHSHGLLVFTGPTGSGKTTTMYALLEV
ARKWQTRRIITLEDPVEQRKDGLLQIQINEKAGITYKTGLKAILRHDPDIILVGEIRDEETAKVAVRASLTGHLVMTTLH
TNDAKGAILRFMDYGITRQEIEQSLLAVAAQRLVELKCPFCRGKCSTLCKSMRQVRQASIYELLYGYELKQAIKEASGEN
VTYHYETLESSVRKGYALGFLEEDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=413047 GSN03_RS20360 WP_212088009.1 3967086..3968129(-) (comGA) [Bacillus nitratireducens strain BM02]
ATGAATAGTGTCGAGCTTTTTGCAAATATGATTATGAAAGAAGCTTGTGGGGTGCAAGCATCGGACTTACATATTGTGCC
CAGGCAGAAGGATATGGCGATTCAATTACGTATAGGAAAAGATTTAATTATGAAACGGTGTATTGAAAAGGAATTTGGAG
AAAAGCTTGTTTCGCACTTTAAATTTTTAGCATCAATGGATATAGGAGAGAGGAGAAAACCTCAAAATGGTTCGTTGTAC
TTGCAAATTGATGGACAAGAAGTGTATTTACGCCTTTCAACACTTCCAACAGTATATCAAGAAAGTCTCGTTATTCGCCT
CCATTTACAAGCATCTTCTCAGCCGTTGTCTCACCTTTCGTTATTTCCAAGTTCAGCGGAAAAATTACTCTCTTTTTTAA
AGCATTCACATGGGTTACTCGTATTTACTGGGCCGACTGGTTCTGGAAAAACAACAACAATGTATGCGTTATTAGAAGTA
GCTAGAAAATGGCAAACACGTCGCATCATTACACTGGAAGATCCAGTTGAGCAAAGAAAAGACGGTTTATTACAAATTCA
AATAAATGAAAAAGCTGGTATCACATATAAAACGGGATTAAAGGCTATTTTGCGTCATGATCCAGATATTATTTTAGTTG
GCGAAATTCGTGATGAAGAAACAGCAAAAGTAGCTGTAAGGGCAAGTTTGACGGGGCATTTAGTAATGACAACCTTGCAT
ACAAATGATGCGAAAGGAGCGATACTACGATTTATGGATTATGGTATTACAAGGCAAGAAATTGAACAATCATTATTAGC
AGTAGCTGCTCAGCGGCTCGTCGAATTAAAATGTCCATTTTGCAGAGGGAAGTGTTCAACTTTATGTAAATCAATGAGGC
AAGTGAGACAGGCAAGTATTTATGAACTGTTATATGGATATGAATTAAAACAAGCGATTAAAGAAGCAAGTGGAGAAAAT
GTTACGTATCACTATGAAACGTTGGAATCGTCGGTTCGAAAAGGGTATGCTTTAGGCTTTTTGGAAGAAGATGTATATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

57.349

100

0.573

Multiple sequence alignment