Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   GSN03_RS19125 Genome accession   NZ_CP047366
Coordinates   3760627..3761061 (-) Length   144 a.a.
NCBI ID   WP_044737687.1    Uniprot ID   -
Organism   Bacillus nitratireducens strain BM02     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3755627..3766061
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GSN03_RS19110 (GSN03_18945) - 3756778..3757335 (+) 558 WP_212087893.1 PadR family transcriptional regulator -
  GSN03_RS19115 (GSN03_18950) metE 3757876..3760164 (+) 2289 WP_212087894.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  GSN03_RS19120 (GSN03_18955) comJ 3760202..3760591 (-) 390 WP_000424027.1 competence protein ComJ -
  GSN03_RS19125 (GSN03_18960) nucA/comI 3760627..3761061 (-) 435 WP_044737687.1 DNA-entry nuclease Machinery gene
  GSN03_RS19130 (GSN03_18965) - 3761298..3762047 (+) 750 WP_000387500.1 DUF3967 domain-containing protein -
  GSN03_RS19135 (GSN03_18970) kinB 3762106..3763380 (-) 1275 WP_212087895.1 sporulation sensor histidine kinase KinB -
  GSN03_RS19140 (GSN03_18975) - 3763690..3763890 (+) 201 WP_000929279.1 hypothetical protein -
  GSN03_RS19145 (GSN03_18980) - 3764008..3765171 (+) 1164 WP_212087896.1 aminotransferase A -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16162.60 Da        Isoelectric Point: 8.4331

>NTDB_id=413044 GSN03_RS19125 WP_044737687.1 3760627..3761061(-) (nucA/comI) [Bacillus nitratireducens strain BM02]
MKQLKGIIISIIAILSLLVAVYEVLMPEETSTKKTTTYDQILEFPKERYPETGKHIADAIKEGHSEMCTIDRNGAADRRK
LSLAPYPTKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFEVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=413044 GSN03_RS19125 WP_044737687.1 3760627..3761061(-) (nucA/comI) [Bacillus nitratireducens strain BM02]
ATGAAGCAATTAAAAGGTATTATCATTTCAATTATTGCAATTCTTTCTCTTTTGGTAGCGGTATATGAAGTACTTATGCC
AGAGGAGACAAGTACGAAAAAAACAACTACGTATGATCAAATTTTAGAGTTCCCGAAAGAACGATATCCAGAGACAGGGA
AACATATAGCGGATGCTATAAAAGAAGGGCATTCAGAAATGTGTACAATTGATCGTAATGGTGCAGCGGATAGAAGGAAA
TTATCGTTAGCGCCATATCCGACGAAGAAAGGTTATGATCGCGATGAATGGCCGATGGCGATGTGTAAGGAAGGCGGAAA
AGGAGCTCATATTGAATATATAAGTCCAGCAGATAACCGCGGTGCAGGTTCTTGGGTAGGGAATAAGCTAGACAAATATC
CAGATGGTACGCGTGTGAAATTTGAAGTGAAATAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(47-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

65

83.333

0.542

Multiple sequence alignment