Detailed information    

insolico Bioinformatically predicted

Overview

Name   recU   Type   Machinery gene
Locus tag   GSN03_RS07940 Genome accession   NZ_CP047366
Coordinates   1543306..1543908 (-) Length   200 a.a.
NCBI ID   WP_000155584.1    Uniprot ID   J8ATF7
Organism   Bacillus nitratireducens strain BM02     
Function   plasmid transformation; homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1538306..1548908
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GSN03_RS07920 (GSN03_07830) dnaD 1538594..1539301 (+) 708 WP_000728545.1 DNA replication protein DnaD -
  GSN03_RS07925 (GSN03_07835) nth 1539317..1539964 (+) 648 WP_000933020.1 endonuclease III -
  GSN03_RS07930 (GSN03_07840) - 1539968..1540486 (+) 519 WP_212089888.1 YpoC family protein -
  GSN03_RS07935 (GSN03_07845) - 1540510..1543239 (-) 2730 WP_212089890.1 penicillin-binding protein 1A -
  GSN03_RS07940 (GSN03_07850) recU 1543306..1543908 (-) 603 WP_000155584.1 Holliday junction resolvase RecU Machinery gene
  GSN03_RS07945 (GSN03_07855) - 1544011..1545036 (+) 1026 WP_212089892.1 DUF2515 domain-containing protein -
  GSN03_RS28650 - 1545338..1545517 (-) 180 WP_328022895.1 phage portal protein -
  GSN03_RS07950 (GSN03_07860) - 1545603..1545845 (+) 243 WP_001252038.1 hypothetical protein -
  GSN03_RS07955 (GSN03_07865) - 1545861..1546205 (+) 345 WP_000872964.1 YppE family protein -
  GSN03_RS07960 (GSN03_07870) - 1546233..1546424 (-) 192 WP_000242654.1 YppF family protein -
  GSN03_RS07965 (GSN03_07875) - 1546602..1547123 (+) 522 WP_097809257.1 YppG family protein -
  GSN03_RS07970 (GSN03_07880) - 1547277..1547699 (+) 423 WP_212089894.1 CotD family spore coat protein -
  GSN03_RS07975 (GSN03_07885) - 1547756..1548310 (+) 555 WP_212089896.1 DUF1273 domain-containing protein -
  GSN03_RS07980 (GSN03_07890) gpsB 1548408..1548743 (+) 336 WP_000622428.1 cell division regulator GpsB -

Sequence

Protein


Download         Length: 200 a.a.        Molecular weight: 23361.62 Da        Isoelectric Point: 9.5601

>NTDB_id=413033 GSN03_RS07940 WP_000155584.1 1543306..1543908(-) (recU) [Bacillus nitratireducens strain BM02]
MTIRYPNGKRYDQASQPHKTPIKKHTYSNRGMSLEEELNETNQYYLTHNIACVHKKPTPLQIVKVDYPARSAAVVKEAYF
KQPSTTDYNGVYKGKYIDFEAKETKNKTSFPLQNFHLHQIEHMKQVVAHNGIAFVIIKFTLFDEFYLLDAKHIIAFWNRQ
NTGGRKSITKQEIEEHGSLLSCGYHPRIDYIRVLDTVYFS

Nucleotide


Download         Length: 603 bp        

>NTDB_id=413033 GSN03_RS07940 WP_000155584.1 1543306..1543908(-) (recU) [Bacillus nitratireducens strain BM02]
ATGACCATTCGTTACCCAAATGGAAAAAGGTACGATCAAGCTTCACAACCTCATAAGACACCAATAAAAAAACATACTTA
CAGTAATAGAGGTATGTCCCTTGAAGAGGAATTGAATGAAACCAATCAATATTACTTAACCCATAATATTGCATGCGTAC
ATAAAAAACCTACACCTCTTCAAATTGTAAAAGTAGATTACCCCGCTCGAAGTGCTGCAGTGGTAAAAGAAGCGTATTTC
AAACAACCTTCTACAACAGATTACAACGGTGTATACAAAGGGAAATACATCGATTTTGAAGCGAAAGAAACTAAAAATAA
AACCAGTTTTCCACTTCAAAACTTCCACCTTCATCAAATTGAACATATGAAGCAAGTAGTCGCTCATAATGGAATTGCAT
TTGTTATTATTAAATTTACACTTTTTGATGAATTTTATTTATTAGATGCAAAACATATTATTGCATTTTGGAATCGTCAA
AATACTGGTGGACGTAAATCGATTACAAAACAAGAAATAGAAGAGCATGGGTCTTTATTATCATGCGGTTATCACCCTCG
AATTGATTATATCCGCGTACTAGACACGGTTTATTTTTCGTGA

Domains

Predicted by InterproScan.

Recombination protein U (RecU)

(30-195)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB J8ATF7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recU Bacillus subtilis subsp. subtilis str. 168

63

100

0.63

Multiple sequence alignment