Detailed information    

insolico Bioinformatically predicted

Overview

Name   priA   Type   Machinery gene
Locus tag   H6S61_RS07460 Genome accession   NZ_CP060047
Coordinates   1482561..1484765 (+) Length   734 a.a.
NCBI ID   WP_185892290.1    Uniprot ID   -
Organism   Vibrio vulnificus strain 2497-87     
Function   DNA puliing through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1477561..1489765
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H6S61_RS07440 (H6S61_07440) - 1478722..1479888 (-) 1167 WP_013571024.1 O-succinylhomoserine (thiol)-lyase -
  H6S61_RS07445 (H6S61_07445) metJ 1480100..1480417 (+) 318 WP_011079338.1 met regulon transcriptional regulator MetJ -
  H6S61_RS07450 (H6S61_07450) - 1480504..1481781 (-) 1278 WP_011079337.1 malic enzyme-like NAD(P)-binding protein -
  H6S61_RS07455 (H6S61_07455) rpmE 1482059..1482280 (-) 222 WP_011151292.1 50S ribosomal protein L31 -
  H6S61_RS07460 (H6S61_07460) priA 1482561..1484765 (+) 2205 WP_185892290.1 primosomal protein N' Machinery gene
  H6S61_RS07465 (H6S61_07465) cytR 1484994..1486001 (+) 1008 WP_011079334.1 DNA-binding transcriptional regulator CytR Regulator
  H6S61_RS07470 (H6S61_07470) - 1486109..1486666 (+) 558 WP_017419640.1 SPOR domain-containing protein -
  H6S61_RS07475 (H6S61_07475) hslV 1486816..1487370 (+) 555 WP_017419639.1 ATP-dependent protease subunit HslV -
  H6S61_RS07480 (H6S61_07480) hslU 1487398..1488729 (+) 1332 WP_011079331.1 HslU--HslV peptidase ATPase subunit -

Sequence

Protein


Download         Length: 734 a.a.        Molecular weight: 82051.62 Da        Isoelectric Point: 8.5813

>NTDB_id=412722 H6S61_RS07460 WP_185892290.1 1482561..1484765(+) (priA) [Vibrio vulnificus strain 2497-87]
MRPSIARVALPVPLDKQFDYLVPGHLFPIIGGRVSVPFGRQTLIGIVTALVQESDFPREQLKPIKAVLDHRPIWPDSVYS
LLLWCSHFYQHPLGETLHNALPAALRKGKAADFATLQEWQLTELGKNQLMQGLNSRAIKQAKVLNMLANGPLPHQTFLDE
EVASTTLRTLEEKGWISCEEKTPQLTRWGNPIEHPVDKPKLNQEQAIAIATVNSQTDFGCYLLEGVTGSGKTEVYLNLIK
PILEQGKQALVLVPEIGLTPQTINRFRQRFDVPVAVIHSGLNDTERLNAWLSARDKAAGIVIGTRSALLIPFSQLGIIIV
DEEHDASYKQQDSLRYHARDVAVMRAAKEGIPIVLGSATPALETLHNALTGKYHHLSLTQRAGMAVPTTNKVLDVKGLYL
ESGLSAPLIAEMRRHLKAGNQVMLFLNRRGFSPALMCHECGWIAECKRCDAYYTYHQYSNEIRCHHCGSQQPIIHQCQGC
GSTQLVTVGVGTEQLENQLTTLFPDYKAIRIDRDSTRRKGSLESALEAIRKGEYQILIGTQMLAKGHHFPDVTLVALLDV
DGSLYSSDFRASERLAQLFIQVAGRAGRASKPGEVILQTHHPEHTLLKALLTKDYRHFAQSALEERKIAQLPPFSFLTLF
RAEANHSEIVETFLRQVRMTLESHPLFDHSCLVLGPTPAPLAKRAGKYRWQLLLQTQHRSLMQKILSSTKPAIDLLPDAK
KVRWNLDIEPQDLS

Nucleotide


Download         Length: 2205 bp        

>NTDB_id=412722 H6S61_RS07460 WP_185892290.1 1482561..1484765(+) (priA) [Vibrio vulnificus strain 2497-87]
ATGCGTCCATCCATTGCTCGTGTTGCGCTGCCAGTGCCACTCGATAAACAATTTGATTATCTCGTTCCTGGACACCTTTT
TCCGATCATTGGTGGCCGCGTGAGTGTGCCGTTTGGACGACAAACCTTGATTGGTATTGTCACCGCTTTGGTTCAAGAAT
CAGACTTTCCACGCGAGCAACTTAAACCCATTAAAGCGGTGTTGGATCATCGTCCTATTTGGCCCGATTCCGTCTACAGT
TTATTACTTTGGTGTAGCCACTTTTACCAACATCCCCTAGGTGAAACACTGCATAACGCCCTCCCCGCTGCACTAAGAAA
AGGCAAAGCGGCTGACTTTGCCACTCTACAAGAATGGCAACTGACCGAGTTAGGCAAAAACCAGTTGATGCAAGGGTTAA
ACAGTCGTGCGATAAAGCAGGCTAAAGTATTAAATATGCTTGCTAATGGCCCTCTGCCTCACCAAACCTTTTTGGATGAG
GAAGTGGCATCCACAACCTTACGCACCTTGGAAGAAAAAGGCTGGATCAGCTGTGAAGAAAAAACGCCACAGTTAACTCG
TTGGGGTAACCCGATTGAACATCCGGTCGATAAACCAAAACTCAACCAAGAGCAAGCCATTGCCATTGCGACCGTTAACA
GCCAGACAGACTTTGGCTGTTATCTGTTGGAAGGGGTCACAGGTTCAGGCAAAACCGAAGTTTATCTCAACTTGATCAAA
CCCATTTTGGAACAAGGTAAACAGGCCCTGGTGTTGGTCCCTGAAATTGGTTTAACACCACAAACCATCAATCGTTTTCG
TCAACGGTTTGATGTGCCAGTCGCCGTCATCCATTCTGGTCTTAATGATACTGAACGTCTCAACGCTTGGCTTTCCGCAC
GAGACAAAGCGGCGGGCATTGTCATTGGTACACGCTCTGCACTACTGATCCCATTTTCCCAGTTAGGGATCATCATTGTC
GATGAAGAGCACGACGCTTCATACAAACAACAAGATAGCCTGCGTTACCATGCTCGCGATGTGGCTGTGATGCGAGCGGC
AAAAGAAGGCATTCCAATTGTTCTCGGTTCAGCCACGCCCGCGTTAGAAACCTTACACAACGCACTTACGGGAAAATATC
ATCATCTCTCGCTGACCCAACGAGCAGGAATGGCCGTGCCAACCACCAATAAAGTGCTGGATGTCAAAGGGCTCTATCTG
GAAAGTGGCCTCTCAGCGCCACTCATTGCTGAAATGCGTCGTCATTTAAAAGCGGGCAATCAGGTGATGCTATTTCTCAA
CCGTCGAGGCTTCTCCCCAGCGTTAATGTGCCATGAATGTGGTTGGATTGCCGAGTGCAAACGCTGCGATGCCTATTACA
CTTATCACCAGTACAGCAATGAAATCCGCTGTCACCACTGTGGCTCTCAACAACCTATTATTCACCAGTGCCAAGGTTGT
GGCTCTACACAACTGGTGACCGTTGGTGTCGGCACTGAGCAACTGGAAAACCAGTTGACGACCCTGTTTCCTGACTACAA
AGCAATTCGCATCGACCGCGATAGCACACGACGCAAAGGTAGCCTCGAAAGCGCTTTAGAAGCCATTCGCAAGGGGGAAT
ACCAAATTTTAATTGGCACGCAAATGCTGGCTAAAGGCCATCATTTCCCAGACGTGACGTTAGTGGCACTATTGGATGTT
GATGGGTCATTATACAGCAGTGACTTCCGAGCGTCCGAGCGATTGGCTCAGTTGTTTATTCAAGTCGCAGGACGAGCAGG
GCGTGCCAGCAAACCTGGCGAAGTGATCTTACAAACCCACCATCCAGAACACACTTTGCTCAAAGCTCTGTTGACCAAGG
ATTACCGGCACTTTGCGCAGTCTGCACTGGAAGAACGGAAAATTGCCCAACTTCCCCCGTTTAGTTTTCTAACGCTATTT
CGCGCAGAAGCCAATCACAGTGAAATCGTCGAAACATTCTTGCGCCAAGTGCGTATGACTCTCGAGTCTCATCCCTTGTT
TGATCACAGTTGTCTCGTGCTCGGCCCAACACCCGCCCCACTGGCCAAACGTGCGGGCAAATACCGCTGGCAGCTTCTGC
TTCAAACTCAACATCGTTCCTTGATGCAGAAAATACTCTCGAGCACGAAACCTGCTATCGATTTGTTACCAGATGCAAAA
AAAGTACGTTGGAACTTGGATATTGAGCCGCAGGATCTGAGTTAG

Domains

Predicted by InterProScan.

DEAD/DEAH box helicase (DEAD)

(205-364)

Helicase conserved C-terminal domain (Helicase_C)

(506-589)

3DNA-binding domain (3BD) (PriA_3primeBD)

(7-102)

PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA)

(446-472)

Primosomal protein N C-terminal domain (PriA_C)

(633-730)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  priA Acinetobacter baumannii D1279779

43.284

100

0.435

Multiple sequence alignment