Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   H6S61_RS07070 Genome accession   NZ_CP060047
Coordinates   1393541..1395265 (-) Length   574 a.a.
NCBI ID   WP_011079397.1    Uniprot ID   A0A3Q0L3L1
Organism   Vibrio vulnificus strain 2497-87     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1388541..1400265
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H6S61_RS07035 (H6S61_07035) - 1388920..1389609 (-) 690 WP_039469689.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  H6S61_RS07040 (H6S61_07040) - 1389609..1390175 (-) 567 WP_185891996.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  H6S61_RS07045 (H6S61_07045) - 1390175..1390657 (-) 483 WP_185891998.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  H6S61_RS07050 (H6S61_07050) - 1390688..1391155 (-) 468 WP_185892000.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  H6S61_RS07055 (H6S61_07055) - 1391189..1391767 (-) 579 WP_011079400.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  H6S61_RS07060 (H6S61_07060) - 1391846..1392307 (-) 462 WP_038964211.1 hypothetical protein -
  H6S61_RS07065 (H6S61_07065) - 1392311..1393531 (-) 1221 WP_185892002.1 type II secretion system F family protein -
  H6S61_RS07070 (H6S61_07070) pilF 1393541..1395265 (-) 1725 WP_011079397.1 GspE/PulE family protein Machinery gene
  H6S61_RS07075 (H6S61_07075) - 1395262..1396383 (-) 1122 WP_039542154.1 tetratricopeptide repeat protein -
  H6S61_RS07080 (H6S61_07080) - 1396380..1397228 (-) 849 WP_038940909.1 AAA family ATPase -
  H6S61_RS07085 (H6S61_07085) mshL 1397236..1398882 (-) 1647 WP_026050229.1 pilus (MSHA type) biogenesis protein MshL -
  H6S61_RS07090 (H6S61_07090) - 1398898..1399218 (-) 321 WP_011079393.1 MSHA biogenesis protein MshK -
  H6S61_RS07095 (H6S61_07095) gspM 1399211..1399861 (-) 651 WP_017420061.1 type II secretion system protein GspM -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 63672.03 Da        Isoelectric Point: 5.3226

>NTDB_id=412713 H6S61_RS07070 WP_011079397.1 1393541..1395265(-) (pilF) [Vibrio vulnificus strain 2497-87]
MKVRLRKRLGDLLVEEGIINEAQLDQALGAQRSGGRKLGDTLIDLGFLSEQQMLAFLSQQLDLPLIDLNRATVDIDAVQL
LPEVHARRLRALVIGRNGDTLRIAMSDPADLFAQEALIGQLGQYGIEFVIAPERQLVEGFDRYYRRTKEIANFAEQLQAE
HQVNDAFDFNIEDDDSDEVTVVKLINSMFEDAIQVGASDIHIEPDANVLRLRQRIDGVLHETLLNEVNIAPALVLRLKLM
ANLDISEKRLPQDGRFNIRSKGQSVDIRMSTMPVQYGESVVMRLLNQSSGVRKLEDSGLPDDLLVRLRRQLKRPHGMILV
TGPTGSGKTTTLYGALTELNQPGKKIITAEDPVEYRLSRVNQVQINPKINLDFSTVLRTFLRQDPDIILIGEMRDHETVE
IGLRAALTGHLVLSTLHTNDAVDSALRMIDMGAPGYLVASAVRAVVAQRLVRRVCPDCKTEDHVGESRKAWLAQRFPNQV
EAGFVKGRGCQNCNLTGYRGRIGVFEMLELEHGMMDALRANDAVEFAHAARRSPGYKPLLASAMELALQGVVSLDEVMNL
GEGDSSGVAEAIIM

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=412713 H6S61_RS07070 WP_011079397.1 1393541..1395265(-) (pilF) [Vibrio vulnificus strain 2497-87]
ATGAAAGTAAGATTAAGAAAGCGGCTTGGTGATCTTCTTGTTGAGGAAGGCATTATCAATGAAGCGCAACTTGATCAAGC
ACTCGGCGCACAAAGAAGTGGTGGGCGTAAGCTTGGCGATACGCTGATTGATTTAGGTTTTTTGTCTGAGCAACAGATGC
TGGCGTTTTTGTCTCAGCAACTTGATCTGCCTTTAATTGACTTAAATCGAGCTACGGTCGATATCGATGCAGTGCAGTTG
CTACCAGAAGTGCATGCTCGTCGTTTACGTGCATTGGTGATAGGCCGTAACGGCGACACGCTGCGCATTGCGATGAGTGA
TCCTGCCGACCTTTTTGCACAAGAAGCGTTGATCGGTCAGCTAGGACAATATGGCATTGAGTTTGTTATCGCGCCAGAAA
GGCAGCTGGTGGAAGGCTTTGATCGCTATTATCGCCGTACCAAAGAGATCGCCAACTTTGCGGAGCAGTTGCAAGCGGAA
CACCAAGTCAACGACGCATTTGATTTCAATATTGAAGATGACGACAGTGATGAGGTGACCGTCGTTAAGCTCATCAACTC
GATGTTTGAAGACGCCATCCAGGTGGGGGCATCGGATATCCATATCGAACCGGATGCCAATGTGTTGCGTTTGCGCCAAC
GTATTGATGGCGTGCTGCACGAAACCTTGCTGAATGAAGTCAACATCGCGCCAGCATTGGTACTGAGATTAAAACTCATG
GCCAACTTGGATATTTCAGAAAAACGTCTGCCGCAAGATGGCCGTTTTAACATCCGCTCCAAAGGTCAGTCGGTGGATAT
CCGTATGTCGACCATGCCAGTTCAGTATGGTGAATCGGTGGTGATGCGTTTGCTCAATCAATCTTCCGGAGTCAGAAAAT
TAGAAGACTCAGGTTTGCCGGACGATCTTTTAGTGCGCCTACGCCGACAGTTGAAACGTCCGCACGGCATGATCCTTGTA
ACTGGCCCTACAGGCTCCGGTAAAACCACCACGCTGTATGGTGCCCTAACAGAATTAAACCAACCGGGGAAAAAGATCAT
CACAGCGGAAGATCCGGTTGAATACCGACTGTCGCGTGTCAACCAAGTGCAGATCAATCCGAAGATCAACCTTGATTTTT
CCACCGTGTTAAGAACGTTCCTCCGTCAGGATCCCGATATCATTTTGATTGGTGAGATGCGTGACCATGAAACGGTTGAA
ATTGGCTTGCGAGCGGCGCTGACCGGTCACTTGGTATTGAGTACCTTACACACCAATGACGCCGTAGACAGTGCGCTTCG
TATGATTGATATGGGTGCTCCGGGTTATCTGGTCGCGAGTGCTGTACGTGCTGTGGTCGCGCAGCGATTGGTGCGCCGTG
TCTGTCCGGATTGCAAAACAGAAGATCACGTTGGCGAGTCACGTAAAGCGTGGTTGGCACAGCGATTCCCGAATCAAGTG
GAAGCAGGCTTTGTGAAAGGGCGCGGTTGTCAAAACTGTAACTTAACAGGTTATCGCGGCCGTATTGGTGTGTTTGAAAT
GCTGGAACTTGAACATGGAATGATGGACGCGTTGCGCGCCAATGATGCCGTCGAGTTTGCGCATGCTGCGCGTCGTTCGC
CTGGCTATAAACCTCTGTTAGCGTCGGCAATGGAGCTGGCGCTACAAGGCGTAGTGAGCTTAGACGAAGTGATGAATTTA
GGCGAAGGTGACTCCTCTGGGGTTGCTGAAGCGATCATTATGTAA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-148)

Type II/IV secretion system protein (T2SSE)

(187-453)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3Q0L3L1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Thermus thermophilus HB27

38.104

97.387

0.371
  pilB Vibrio cholerae strain A1552

37.699

98.432

0.371