Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   H5022_RS01890 Genome accession   NZ_CP060004
Coordinates   413751..415097 (+) Length   448 a.a.
NCBI ID   WP_003160919.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain WK172     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 408751..420097
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H5022_RS01870 (H5022_01865) - 409588..410154 (-) 567 WP_003084491.1 hypothetical protein -
  H5022_RS01875 (H5022_01870) rsmD 410157..410753 (-) 597 WP_003102864.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  H5022_RS01880 (H5022_01875) - 410753..412240 (-) 1488 WP_003102862.1 M16 family metallopeptidase -
  H5022_RS01885 (H5022_01880) - 412233..413630 (-) 1398 WP_003084498.1 M16 family metallopeptidase -
  H5022_RS01890 (H5022_01885) pilA 413751..415097 (+) 1347 WP_003160919.1 signal recognition particle-docking protein FtsY Machinery gene
  H5022_RS01895 (H5022_01890) ftsE 415094..415765 (+) 672 WP_003084505.1 cell division ATP-binding protein FtsE -
  H5022_RS01900 (H5022_01895) ftsX 415765..416772 (+) 1008 WP_003084508.1 permease-like cell division protein FtsX -
  H5022_RS01905 (H5022_01900) rpoH 416886..417740 (+) 855 WP_003084510.1 RNA polymerase sigma factor RpoH -
  H5022_RS01910 (H5022_01905) - 417805..418410 (+) 606 WP_003084511.1 hypothetical protein -
  H5022_RS01915 (H5022_01910) mtgA 418415..419146 (-) 732 WP_003100972.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  H5022_RS01920 (H5022_01915) - 419183..419560 (+) 378 WP_003121748.1 DUF423 domain-containing protein -
  H5022_RS01925 (H5022_01920) thiS 419663..419863 (+) 201 WP_003084514.1 sulfur carrier protein ThiS -

Sequence

Protein


Download         Length: 448 a.a.        Molecular weight: 47646.03 Da        Isoelectric Point: 4.6083

>NTDB_id=412265 H5022_RS01890 WP_003160919.1 413751..415097(+) (pilA) [Pseudomonas aeruginosa strain WK172]
MFGSNDDKKAPQGGEKKGLFGWWRKKPQAGEQPADQPVEPVSETAAAEQRAPADDVAQSLTEQPGRQQPSAAEPAEPAPV
AEAPLASDEPASAEEHSPRPEAPVAQPEPILAAEPEPEPEPEPVAPLAAAPAVSEPATRPGFFARLRQGLSKTSASIGEG
MASLFLGRKEIDDDLLDDIETRLLTADVGVEATTLIVQNLTKRVARKELADSGALYKALQEELASLLRPVEQPLQVDVAR
EPYVILVVGVNGVGKTTTIGKLAKKLQLEGKKVMLAAGDTFRAAAVEQLQVWGERNRIPVIAQHTGADSASVIFDAVQAA
KARGIDVLIADTAGRLHTKDNLMEELKKVRRVIGKLDETAPHEVLLVLDAGTGQNAINQAKQFNLAVELTGLALTKLDGT
AKGGVIFALAKQFGLPIRYIGVGEGIDDLRTFEADAFVQALFAERENA

Nucleotide


Download         Length: 1347 bp        

>NTDB_id=412265 H5022_RS01890 WP_003160919.1 413751..415097(+) (pilA) [Pseudomonas aeruginosa strain WK172]
ATGTTTGGTTCCAACGACGACAAGAAGGCCCCGCAGGGCGGGGAAAAGAAAGGTCTGTTCGGCTGGTGGCGGAAAAAGCC
GCAAGCCGGCGAGCAGCCTGCCGATCAGCCTGTCGAGCCGGTCTCCGAGACAGCGGCCGCCGAGCAGCGCGCGCCTGCCG
ATGACGTGGCGCAATCCCTGACAGAACAGCCAGGTCGGCAGCAGCCCTCCGCTGCCGAGCCGGCCGAGCCGGCGCCGGTT
GCCGAAGCACCGCTCGCCAGTGACGAGCCAGCGTCGGCCGAGGAGCATTCGCCCCGGCCCGAGGCGCCCGTCGCGCAACC
CGAGCCTATCCTGGCTGCCGAGCCCGAGCCCGAGCCCGAGCCCGAGCCCGTTGCGCCGCTGGCCGCCGCTCCGGCAGTCA
GCGAGCCGGCGACCAGGCCGGGCTTCTTCGCCCGCCTCCGGCAGGGACTGTCCAAGACCAGCGCCAGCATCGGCGAAGGC
ATGGCCAGCCTGTTCCTCGGTCGCAAGGAAATCGACGACGACCTGCTCGACGACATCGAGACGCGCCTGCTGACCGCCGA
CGTCGGCGTCGAGGCCACCACCCTGATCGTGCAGAACCTGACCAAGCGCGTGGCGCGCAAGGAGCTGGCCGACAGCGGCG
CGCTGTACAAGGCGCTGCAGGAAGAGCTGGCCAGCCTGCTGCGCCCGGTGGAGCAGCCGTTGCAGGTCGACGTCGCGCGC
GAGCCCTATGTGATCCTGGTGGTCGGGGTGAACGGCGTGGGCAAGACCACCACCATCGGCAAGCTGGCGAAGAAGCTGCA
ACTCGAGGGCAAGAAGGTCATGCTGGCCGCCGGCGACACCTTCCGCGCCGCCGCCGTGGAGCAGTTGCAGGTCTGGGGCG
AGCGCAACCGCATCCCGGTGATCGCCCAGCACACCGGCGCCGACTCCGCCTCGGTGATCTTCGACGCGGTGCAGGCCGCC
AAGGCCCGCGGCATCGACGTGCTGATCGCCGATACCGCCGGACGCCTGCATACCAAGGACAACCTGATGGAGGAGCTGAA
GAAGGTGCGCCGGGTGATCGGCAAGCTGGACGAGACGGCGCCCCATGAGGTCCTGCTGGTGCTCGACGCCGGCACCGGGC
AGAACGCCATCAACCAGGCCAAGCAGTTCAACCTCGCGGTGGAGCTGACCGGCCTTGCCCTGACCAAACTGGACGGCACC
GCCAAGGGCGGGGTGATCTTCGCCCTGGCCAAGCAGTTCGGCCTGCCGATCCGCTATATCGGTGTCGGCGAGGGGATCGA
CGATCTCCGTACCTTCGAGGCCGACGCCTTCGTCCAGGCACTCTTCGCAGAGCGGGAGAACGCATGA

Domains

Predicted by InterProScan.

SRP54-type protein, helical bundle domain (SRP54_N)

(158-223)

SRP54-type protein, GTPase domain (SRP54)

(243-442)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

47.907

95.982

0.46

Multiple sequence alignment