Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   N026_RS22305 Genome accession   NZ_CP047267
Coordinates   5016649..5018016 (-) Length   455 a.a.
NCBI ID   WP_002555235.1    Uniprot ID   A0A108WX83
Organism   Pseudomonas syringae UB303     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 5011649..5023016
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N026_RS22285 (N026_22285) yjiA 5012615..5013592 (-) 978 WP_011268931.1 GTPase -
  N026_RS22290 (N026_22290) - 5013688..5013885 (-) 198 WP_003399964.1 YbdD/YjiX family protein -
  N026_RS22295 (N026_22295) - 5013913..5015988 (-) 2076 WP_011268932.1 carbon starvation CstA family protein -
  N026_RS22300 (N026_22300) - 5016274..5016642 (+) 369 WP_024661089.1 PilZ domain-containing protein -
  N026_RS22305 (N026_22305) radA 5016649..5018016 (-) 1368 WP_002555235.1 DNA repair protein RadA Machinery gene
  N026_RS22310 (N026_22310) mscL 5018213..5018659 (+) 447 WP_003399960.1 large-conductance mechanosensitive channel protein MscL -
  N026_RS22315 (N026_22315) - 5019245..5020021 (-) 777 WP_011268934.1 ferredoxin--NADP reductase -
  N026_RS22320 (N026_22320) - 5020358..5020591 (+) 234 WP_003340758.1 helix-turn-helix domain-containing protein -
  N026_RS22325 (N026_22325) - 5020722..5021846 (+) 1125 WP_003399957.1 methyltransferase -
  N026_RS22330 (N026_22330) - 5021934..5022101 (-) 168 WP_002555239.1 DUF2474 domain-containing protein -

Sequence

Protein


Download         Length: 455 a.a.        Molecular weight: 48913.48 Da        Isoelectric Point: 7.1316

>NTDB_id=411965 N026_RS22305 WP_002555235.1 5016649..5018016(-) (radA) [Pseudomonas syringae UB303]
MAKAKRLYGCTECGATFPKWAGQCSECGAWNTLVETMIESGAAAPPTGRTGWTGSQAQIRTLAEVSVEEIPRFSTNSTEL
DRVLGGGLVDGSVVLIGGDPGIGKSTILLQTLCNIAQRMPALYVTGEESQQQVAMRARRLGLPQDKLRVMTETCIETIIA
TAKVEKPKVMVIDSIQTIFTEQLQSAPGGVSQVRESAALLVRYAKQSGTAIFLVGHVTKEGALAGPRVLEHMVDTVLYFE
GESDGRLRLLRAVKNRFGAVNELGVFGMTDKGLKEVSNPSAIFLTRAQEEVPGSVVMATWEGTRPMLVEVQALVDDSHMS
NPRRVTLGLDQNRLAMLLAVLHRHGGIPTHDQDVFLNVVGGVKVLETASDLALMAAVMSSLRNRPLPHDLLVFGEVGLSG
EVRPVPSGQERLKEAAKHGFKRAIVPKGNAPKEAPPGLQIIAVTRLEQALDALFE

Nucleotide


Download         Length: 1368 bp        

>NTDB_id=411965 N026_RS22305 WP_002555235.1 5016649..5018016(-) (radA) [Pseudomonas syringae UB303]
ATGGCCAAGGCCAAACGCTTGTACGGCTGCACCGAGTGCGGCGCGACATTTCCCAAGTGGGCGGGCCAGTGCAGCGAATG
CGGTGCCTGGAATACACTGGTCGAAACCATGATTGAAAGCGGTGCCGCCGCGCCGCCCACCGGGCGTACCGGCTGGACCG
GTTCGCAGGCGCAGATCAGGACCCTGGCGGAGGTCAGCGTCGAAGAAATCCCGCGCTTCTCCACCAACTCCACCGAGCTG
GACAGGGTGCTCGGCGGCGGTCTGGTTGACGGTTCGGTGGTGCTGATTGGCGGCGACCCTGGCATCGGCAAGTCCACGAT
CCTCTTGCAGACGCTGTGCAACATCGCCCAGCGCATGCCCGCGCTGTATGTCACCGGTGAGGAATCCCAGCAGCAGGTCG
CCATGCGCGCCCGGCGCCTGGGGCTGCCGCAGGACAAGCTGCGGGTCATGACCGAAACCTGCATCGAGACCATCATTGCC
ACCGCCAAGGTCGAGAAGCCCAAGGTGATGGTGATCGATTCGATCCAGACGATTTTCACCGAACAGCTGCAGTCGGCCCC
TGGCGGCGTGTCGCAGGTTCGCGAAAGCGCCGCCTTGCTGGTGCGCTACGCCAAGCAGAGCGGTACGGCGATCTTCCTGG
TCGGCCACGTCACCAAGGAAGGTGCACTGGCCGGCCCGCGTGTGCTTGAGCACATGGTCGACACCGTGTTGTATTTCGAG
GGCGAATCGGACGGCCGCCTGCGTTTGCTGCGCGCGGTGAAGAACCGCTTTGGCGCGGTCAACGAGCTGGGCGTGTTCGG
CATGACCGACAAGGGCCTGAAAGAAGTCTCCAACCCGTCGGCGATCTTTCTCACCCGTGCGCAGGAAGAAGTACCGGGCA
GTGTGGTCATGGCCACTTGGGAGGGCACGCGGCCGATGCTGGTCGAGGTGCAGGCGCTGGTGGACGACAGTCACATGTCC
AATCCGCGCCGCGTAACGCTGGGTCTGGATCAGAATCGTCTGGCCATGTTGCTGGCGGTCCTGCACCGACATGGCGGCAT
TCCCACTCACGATCAGGACGTGTTTCTCAACGTAGTGGGTGGTGTGAAGGTGCTGGAAACCGCCTCCGACCTGGCCTTGA
TGGCAGCGGTGATGTCCAGCCTGCGCAATCGTCCGTTGCCGCATGACTTGCTGGTGTTCGGTGAGGTTGGGCTGTCGGGT
GAAGTCAGGCCGGTGCCCAGCGGCCAGGAGCGCCTCAAGGAGGCCGCCAAGCACGGCTTCAAGCGCGCCATCGTGCCCAA
GGGCAATGCGCCGAAGGAAGCGCCGCCCGGCTTGCAGATCATTGCCGTTACGCGCCTGGAGCAGGCCCTGGATGCGCTGT
TCGAGTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(73-221)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(353-429)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A108WX83

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

49.123

100

0.492
  radA Streptococcus pneumoniae Rx1

46.739

100

0.473
  radA Streptococcus pneumoniae R6

46.739

100

0.473
  radA Streptococcus pneumoniae TIGR4

46.739

100

0.473
  radA Streptococcus pneumoniae D39

46.739

100

0.473
  radA Streptococcus mitis SK321

46.93

100

0.47
  radA Streptococcus mitis NCTC 12261

46.711

100

0.468

Multiple sequence alignment