Detailed information    

insolico Bioinformatically predicted

Overview

Name   priA   Type   Machinery gene
Locus tag   N026_RS01715 Genome accession   NZ_CP047267
Coordinates   348921..351140 (-) Length   739 a.a.
NCBI ID   WP_024660643.1    Uniprot ID   -
Organism   Pseudomonas syringae UB303     
Function   DNA puliing through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 343921..356140
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N026_RS01695 (N026_01695) hslU 344038..345375 (-) 1338 WP_003316641.1 ATP-dependent protease ATPase subunit HslU -
  N026_RS01700 (N026_01700) hslV 345442..345972 (-) 531 WP_003392345.1 ATP-dependent protease subunit HslV -
  N026_RS01705 (N026_01705) - 346387..347082 (-) 696 WP_003403056.1 SPOR domain-containing protein -
  N026_RS01710 (N026_01710) argS 347084..348823 (-) 1740 WP_024660642.1 arginine--tRNA ligase -
  N026_RS01715 (N026_01715) priA 348921..351140 (-) 2220 WP_024660643.1 primosomal protein N' Machinery gene
  N026_RS01720 (N026_01720) rpmE 351310..351531 (+) 222 WP_003403048.1 50S ribosomal protein L31 -
  N026_RS01725 (N026_01725) - 351545..352348 (+) 804 WP_024650769.1 thermonuclease family protein -
  N026_RS01730 (N026_01730) - 352530..353798 (+) 1269 WP_024650770.1 malic enzyme-like NAD(P)-binding protein -

Sequence

Protein


Download         Length: 739 a.a.        Molecular weight: 81928.23 Da        Isoelectric Point: 8.5469

>NTDB_id=411941 N026_RS01715 WP_024660643.1 348921..351140(-) (priA) [Pseudomonas syringae UB303]
MPDAILRLALPSPLRRLFDYRAPAGVSRSALQPGMRLRVPFGRREMIGILVEVVDHSEVPADKLKPAIALLDSEAPLPPA
LFKLCLWTSQYYQHSLGDTLSWALPVLLRQGELAESRQERFWHVAPGASVDDPRIARAPKQREALTTLAQHPHGVAHQLL
SKLMLNRDSLNLLLAKELVYVEVRSHAPSARHEHWLAQPELPLNTEQRAAYEAIRAGFDSFHAFLLAGVTGSGKTEVYLQ
LIRETLEAGKQALVLIPEINLGPQTLARFEQRFNARIALVHSAVNDRERLDAWLAARDGEADIIIGTRSALFTPMKNPGL
IIIDEEHDGSYKQQEGLRYHARDLALVRARQENIPIVLGSATPSLESLHNAYTGRYGLLRLNQRAGGAQQPRFMRLDVKS
RPLDSGISGPMQQAIGQTLAAGQQVLVFLNRRGFAPTLLCHDCGWMSGCQRCDARMTVHQRSGELRCHHCGYVERVPRQC
PSCGKVDLRPVGAGTERAEERLAILFPDYPVLRVDRDSTSRKDAMNQLFTTIQRGQPCILVGTQMLAKGHHFPRVTLVSI
LDADGGLFSGDFRASERMAQLIVQVAGRAGRAEEPGKVIIQTHLADHPLLIQLTEQGYFAFAEQALSERRSASLPPFSHL
ALLRAEAHKPGQAEAFLDQACSDAEQLVAQMALGGIELLGPVPAPMERRAGRYRAQLLVQSSARAPLHKLLATWLLALEQ
MPSGRQVRWSLDVDPVDLY

Nucleotide


Download         Length: 2220 bp        

>NTDB_id=411941 N026_RS01715 WP_024660643.1 348921..351140(-) (priA) [Pseudomonas syringae UB303]
GTGCCCGACGCCATCCTGCGCCTTGCCCTGCCCTCGCCGCTTCGCCGCCTGTTCGACTACCGCGCCCCCGCGGGCGTGTC
GCGCAGCGCCTTGCAGCCGGGCATGCGTCTGCGAGTGCCGTTCGGACGTCGGGAAATGATCGGCATTCTGGTGGAAGTGG
TCGATCACAGCGAAGTACCCGCCGACAAGCTGAAGCCGGCTATCGCCCTGCTCGACAGTGAAGCGCCGCTGCCGCCCGCA
CTGTTCAAGCTGTGCCTGTGGACCTCGCAGTACTACCAGCACAGCCTCGGAGACACACTGAGCTGGGCCTTGCCGGTGCT
GCTGCGTCAGGGCGAACTGGCCGAAAGTCGCCAAGAGCGCTTCTGGCATGTGGCCCCGGGCGCCAGCGTCGACGATCCGC
GCATCGCCCGCGCGCCCAAGCAGCGCGAAGCCCTGACCACCCTTGCCCAGCACCCGCACGGAGTGGCGCATCAACTGCTC
AGCAAGCTGATGCTTAACAGGGACAGCCTCAACCTGTTGCTGGCCAAGGAGCTGGTGTACGTCGAGGTCCGCAGCCATGC
GCCCAGCGCACGCCATGAACACTGGCTGGCCCAGCCTGAATTGCCACTCAACACCGAACAACGCGCCGCTTACGAAGCGA
TCCGCGCCGGATTCGACAGCTTTCATGCGTTTCTGCTGGCCGGTGTCACCGGCAGCGGCAAGACCGAAGTCTATCTGCAA
CTGATCCGCGAAACCCTCGAAGCCGGCAAGCAGGCGCTGGTGCTGATCCCGGAAATCAACCTCGGCCCGCAGACCCTGGC
GCGCTTCGAGCAGCGCTTCAATGCGCGCATTGCGCTGGTCCACTCGGCGGTCAACGACCGCGAACGGCTGGACGCCTGGC
TGGCGGCTCGCGATGGCGAGGCGGACATCATCATCGGCACGCGTTCGGCGCTGTTCACGCCGATGAAAAACCCCGGCCTG
ATCATCATCGACGAAGAACACGACGGTTCCTATAAACAGCAGGAAGGTCTGCGCTATCACGCCCGCGATCTGGCACTGGT
GCGGGCGCGTCAGGAAAACATTCCGATTGTCCTGGGCTCGGCCACGCCGTCCCTGGAAAGCCTGCACAACGCCTACACCG
GCCGCTATGGCCTGTTGCGCCTGAATCAGCGCGCGGGCGGCGCACAACAGCCGCGTTTCATGCGCCTGGACGTCAAGAGC
CGACCGCTGGACAGCGGCATCTCCGGGCCCATGCAGCAGGCCATCGGCCAGACGCTCGCGGCAGGCCAGCAGGTCTTGGT
GTTCCTCAACCGTCGCGGCTTTGCGCCAACCTTGCTGTGCCACGACTGCGGCTGGATGTCCGGCTGCCAACGCTGCGATG
CACGCATGACCGTGCACCAGCGCTCGGGCGAACTGCGCTGCCATCACTGCGGGTATGTCGAGCGCGTACCGCGGCAGTGC
CCGTCGTGCGGCAAGGTGGATTTGCGCCCGGTAGGCGCCGGCACCGAGCGCGCCGAAGAACGCCTGGCGATTCTGTTTCC
GGACTACCCCGTGCTGCGTGTCGATCGCGACAGCACGTCGCGCAAGGACGCCATGAACCAGCTGTTCACGACGATCCAGC
GCGGTCAGCCGTGCATTCTGGTCGGCACGCAAATGCTCGCCAAGGGCCATCACTTTCCCCGGGTCACCCTGGTCTCGATT
CTGGACGCCGACGGCGGGCTGTTTTCCGGCGACTTCCGCGCCAGCGAGCGCATGGCGCAACTGATCGTGCAGGTGGCAGG
CCGTGCCGGGCGCGCCGAAGAGCCTGGCAAGGTGATCATCCAGACCCACCTGGCCGACCATCCGTTGCTGATCCAGCTGA
CCGAGCAGGGTTATTTCGCCTTTGCCGAACAAGCCTTGAGCGAGCGCCGTTCAGCGAGTCTGCCACCGTTTTCACACCTT
GCACTGCTGCGCGCCGAAGCGCACAAGCCGGGACAGGCCGAGGCCTTTCTCGATCAGGCCTGCAGCGATGCCGAGCAACT
GGTTGCGCAAATGGCGCTGGGCGGCATAGAGCTGCTGGGCCCGGTTCCGGCCCCGATGGAACGCCGCGCCGGCCGTTATC
GCGCGCAACTGCTGGTGCAGTCCAGCGCCCGCGCGCCACTGCATAAATTGCTTGCCACCTGGCTGCTCGCACTGGAGCAA
ATGCCCAGCGGTCGACAGGTCAGATGGTCGCTGGATGTGGATCCCGTGGATCTTTATTAA

Domains

Predicted by InterproScan.

3DNA-binding domain (3BD) (PriA_3primeBD)

(9-105)

Primosomal protein N C-terminal domain (PriA_C)

(636-735)

DEAD/DEAH box helicase (DEAD)

(207-367)

PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA)

(449-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  priA Acinetobacter baumannii D1279779

47.844

100

0.48

Multiple sequence alignment