Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   GSR16_RS13995 Genome accession   NZ_CP047241
Coordinates   3082787..3084151 (-) Length   454 a.a.
NCBI ID   WP_159878398.1    Uniprot ID   -
Organism   Aquitalea denitrificans strain 5YN1-3     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3077787..3089151
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GSR16_RS13960 - 3077918..3078130 (-) 213 WP_089083135.1 hypothetical protein -
  GSR16_RS13965 - 3078206..3078973 (-) 768 WP_159878386.1 C40 family peptidase -
  GSR16_RS13970 - 3079034..3080716 (+) 1683 WP_159878388.1 ABC1 kinase family protein -
  GSR16_RS13975 - 3080716..3081495 (+) 780 WP_159878390.1 hypothetical protein -
  GSR16_RS13980 - 3081621..3081974 (+) 354 WP_159878392.1 DUF2325 domain-containing protein -
  GSR16_RS13985 - 3082048..3082227 (-) 180 WP_159878394.1 zf-HC2 domain-containing protein -
  GSR16_RS13990 - 3082224..3082784 (-) 561 WP_159878396.1 sigma-70 family RNA polymerase sigma factor -
  GSR16_RS13995 radA 3082787..3084151 (-) 1365 WP_159878398.1 DNA repair protein RadA Machinery gene
  GSR16_RS14000 - 3084175..3084765 (-) 591 WP_159878400.1 hypothetical protein -
  GSR16_RS14005 - 3084823..3085581 (-) 759 WP_159878402.1 SDR family oxidoreductase -
  GSR16_RS14010 hpnE 3085578..3086906 (-) 1329 WP_159878404.1 hydroxysqualene dehydroxylase HpnE -
  GSR16_RS14015 hpnD 3086914..3087759 (-) 846 WP_159878406.1 presqualene diphosphate synthase HpnD -
  GSR16_RS14020 hpnC 3087825..3088637 (-) 813 WP_159878408.1 squalene synthase HpnC -

Sequence

Protein


Download         Length: 454 a.a.        Molecular weight: 48949.42 Da        Isoelectric Point: 7.1459

>NTDB_id=411791 GSR16_RS13995 WP_159878398.1 3082787..3084151(-) (radA) [Aquitalea denitrificans strain 5YN1-3]
MAKIKTVFSCTECGGQVPKWQGQCPHCSAWNTLVEAVSTPTASNPRFQSWSTTNARVQVLSEVKTEEVPRDPSGIDELDR
VLGGGIVRGAVILIGGDPGIGKSTLLLQALAVIGKRRKVLYVSGEESPQQIALRASRLAVDPSQVNLLAEIRLEAIQEVL
KQEQPDVAVIDSIQTLYTEQVTSAPGSVSQVRECAAQLTRMAKQTGTTILLVGHVTKEGSLAGPRVLEHMVDTVLYFEGD
SHSSHRMIRAIKNRFGAVNELGVFVMTETGLKGVSNPSAIFLSSYRDDVPGSCVLVTQEGTRPLLVEVQALVDDCHGMQP
KRLTVGLEQNRLAMLLAVLHRHGGVACFDQDVFLNAVGGVKISEPAADLAVILAMVSSLRNKPLPEKLVVFGEVGLAGEV
RPVSRGQERLKEAAKLGFTRALIPTANKPRQPIEGLQIITVDRLEHAVDFCRGD

Nucleotide


Download         Length: 1365 bp        

>NTDB_id=411791 GSR16_RS13995 WP_159878398.1 3082787..3084151(-) (radA) [Aquitalea denitrificans strain 5YN1-3]
ATGGCAAAAATAAAAACTGTTTTCAGCTGCACCGAGTGCGGCGGCCAAGTGCCGAAATGGCAGGGCCAGTGTCCGCATTG
TTCCGCCTGGAACACCCTGGTCGAGGCAGTATCCACGCCAACCGCCAGCAATCCGCGTTTCCAATCCTGGTCCACCACCA
ATGCCCGCGTGCAAGTGCTGTCCGAGGTCAAGACCGAAGAAGTCCCGCGTGACCCTTCCGGCATCGACGAACTGGACCGC
GTGCTGGGCGGCGGCATCGTGCGTGGTGCTGTCATCCTGATTGGCGGCGACCCTGGCATTGGCAAATCCACCTTGCTGTT
GCAGGCGCTGGCGGTGATCGGCAAGCGGCGCAAGGTGCTGTATGTGTCGGGCGAAGAATCGCCGCAGCAGATTGCCCTGC
GTGCCTCGCGGCTGGCAGTAGACCCCAGCCAGGTCAATCTGTTGGCGGAAATCCGTCTGGAGGCCATTCAGGAAGTCCTG
AAACAGGAACAACCGGATGTAGCGGTGATCGACTCCATCCAGACCCTGTACACCGAGCAGGTGACCTCGGCACCAGGGTC
GGTGTCCCAGGTGCGCGAATGTGCGGCCCAGCTCACCCGCATGGCCAAGCAGACCGGCACCACCATCTTGCTGGTAGGCC
ATGTCACCAAGGAAGGCTCGCTGGCCGGCCCGCGCGTGCTGGAACACATGGTGGACACCGTGCTGTATTTCGAGGGAGAT
AGCCACTCCAGCCACCGCATGATCCGTGCCATCAAGAACCGCTTTGGCGCGGTCAACGAGCTGGGCGTGTTCGTGATGAC
CGAAACCGGGCTCAAGGGGGTGTCCAATCCCTCGGCCATTTTCCTGTCGTCTTACCGGGACGATGTGCCCGGTTCTTGCG
TACTGGTGACGCAGGAAGGCACGCGCCCCTTGCTGGTGGAGGTGCAGGCGCTGGTGGATGACTGTCACGGCATGCAGCCC
AAGCGCCTGACCGTGGGTCTGGAGCAGAACCGGCTGGCCATGCTGCTGGCGGTGCTGCATCGCCATGGCGGTGTGGCCTG
CTTTGATCAGGATGTATTCCTGAACGCCGTGGGCGGGGTCAAGATCAGCGAACCGGCTGCCGACCTGGCCGTCATCCTGG
CCATGGTGTCATCCCTGCGCAACAAGCCGCTGCCGGAAAAACTGGTGGTGTTTGGTGAAGTGGGGCTGGCCGGCGAGGTG
CGTCCGGTATCCCGTGGGCAGGAGCGGCTGAAAGAAGCGGCCAAGCTGGGCTTTACCCGGGCGCTGATTCCTACTGCCAA
CAAGCCGCGCCAGCCGATTGAGGGTCTGCAGATCATTACTGTGGACCGGCTGGAGCATGCCGTCGATTTTTGTCGCGGAG
ACTGA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(67-219)

Lon protease (S16) C-terminal proteolytic domain (Lon_C)

(360-431)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

48.904

100

0.491
  radA Streptococcus mitis NCTC 12261

47.321

98.678

0.467
  radA Streptococcus pneumoniae Rx1

49.057

93.392

0.458
  radA Streptococcus pneumoniae D39

49.057

93.392

0.458
  radA Streptococcus pneumoniae R6

49.057

93.392

0.458
  radA Streptococcus pneumoniae TIGR4

49.057

93.392

0.458
  radA Streptococcus mitis SK321

49.057

93.392

0.458

Multiple sequence alignment