Detailed information    

insolico Bioinformatically predicted

Overview

Name   rapF   Type   Regulator
Locus tag   GRT08_RS17860 Genome accession   NZ_CP047234
Coordinates   3618818..3619963 (+) Length   381 a.a.
NCBI ID   WP_079890910.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain FJ4     
Function   inhibit the DNA-binding function of ComA (predicted from homology)   
Competence regulation

Genomic Context

Location: 3613818..3624963
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GRT08_RS17840 (GRT08_17605) - 3614715..3615143 (-) 429 WP_032869448.1 DUF1934 domain-containing protein -
  GRT08_RS17845 (GRT08_17610) - 3615297..3615809 (+) 513 Protein_3454 bacteriocin biosynthesis protein AlbG -
  GRT08_RS17850 (GRT08_17615) - 3615816..3617192 (-) 1377 WP_156735434.1 YncE family protein -
  GRT08_RS17855 (GRT08_17620) - 3617232..3618587 (-) 1356 WP_377802885.1 YncE family protein -
  GRT08_RS17860 (GRT08_17625) rapF 3618818..3619963 (+) 1146 WP_079890910.1 tetratricopeptide repeat protein Regulator
  GRT08_RS17865 (GRT08_17630) phrF 3619947..3620066 (+) 120 WP_069473622.1 PhrC/PhrF family phosphatase-inhibitory pheromone Regulator
  GRT08_RS17870 (GRT08_17635) - 3620265..3620468 (+) 204 WP_003151064.1 helix-turn-helix transcriptional regulator -
  GRT08_RS17875 (GRT08_17640) - 3620482..3620685 (+) 204 WP_069473623.1 hypothetical protein -
  GRT08_RS17880 (GRT08_17645) - 3620720..3620905 (+) 186 Protein_3461 DUF4177 domain-containing protein -
  GRT08_RS17885 (GRT08_17650) - 3621093..3621374 (+) 282 WP_014306021.1 hypothetical protein -
  GRT08_RS17890 (GRT08_17655) - 3621418..3621930 (+) 513 WP_069473625.1 stage II sporulation protein M -
  GRT08_RS17895 (GRT08_17665) - 3622521..3622739 (-) 219 WP_003151056.1 LuxR C-terminal-related transcriptional regulator -
  GRT08_RS17900 (GRT08_17670) speB 3622909..3623781 (-) 873 WP_003151054.1 agmatinase -
  GRT08_RS17905 (GRT08_17675) speE 3623841..3624671 (-) 831 WP_003151052.1 spermidine synthase -

Sequence

Protein


Download         Length: 381 a.a.        Molecular weight: 45571.68 Da        Isoelectric Point: 4.5378

>NTDB_id=411718 GRT08_RS17860 WP_079890910.1 3618818..3619963(+) (rapF) [Bacillus amyloliquefaciens strain FJ4]
MASVLSSSAIGEKINEWYMYIRRFSIPDAEYLRREIKEELAGLEDDQDLHLYYSLMEFRHNLMLEYLEPLESQRIEEQPR
LSDLLADIDKKQARLTGRLDYYFNFFRGMYELECREYLSAIQFFKKAEYKLDFVKDCIEKAEFYFKMSESYYYMKQTYFS
MDYARQAYKIYHKQEAYNIRVLQCHSLFATNFLDLKQYDEAIQHFKKAYAMAEAEQQPQLMGRTLYNIGLCFNSQGNYKP
AIDYIKQAIAVFEDGNIITSLPQAYFLITQIHYKIGNMAIARQYHDKGVSYAEEAEDSLYIVEYEFLESLYVDEPDEEAI
MECFDFLRDKMMYADLEDFALDVAKYYHERENFEKAAAYFLKVEETRQQIQGGVKLYEIEV

Nucleotide


Download         Length: 1146 bp        

>NTDB_id=411718 GRT08_RS17860 WP_079890910.1 3618818..3619963(+) (rapF) [Bacillus amyloliquefaciens strain FJ4]
GTGGCGAGTGTTTTATCGTCTTCAGCGATTGGAGAAAAAATCAACGAATGGTATATGTACATTCGAAGATTCAGCATCCC
GGACGCCGAGTACTTGCGGAGAGAAATCAAAGAAGAACTTGCAGGCCTGGAAGATGACCAGGATCTTCACCTGTACTATT
CTCTCATGGAATTCCGCCACAATCTGATGCTCGAATACCTCGAACCGCTGGAATCGCAGCGGATAGAAGAACAGCCGAGA
CTATCGGATCTGCTCGCGGATATTGATAAAAAGCAGGCCCGGCTGACCGGACGGCTTGATTATTATTTTAATTTTTTCCG
GGGGATGTATGAGCTGGAATGCCGTGAATATCTTTCAGCGATTCAGTTTTTTAAGAAAGCTGAATATAAGCTGGATTTTG
TCAAAGATTGTATCGAGAAAGCTGAATTTTATTTTAAGATGTCAGAGTCCTACTATTATATGAAGCAGACTTATTTTTCT
ATGGATTATGCCCGGCAGGCTTATAAAATTTATCATAAGCAGGAGGCATATAATATAAGGGTGCTTCAGTGCCATTCATT
GTTCGCTACCAACTTTCTTGACTTAAAGCAATATGATGAAGCAATTCAGCATTTTAAAAAAGCTTATGCCATGGCAGAGG
CAGAACAGCAGCCTCAATTAATGGGCCGGACTCTATATAATATCGGACTTTGTTTTAACAGCCAAGGAAATTACAAACCA
GCCATTGATTACATAAAACAGGCAATAGCTGTTTTTGAAGATGGGAACATCATCACTTCTCTCCCGCAAGCCTATTTCTT
AATCACACAAATACATTATAAAATCGGAAATATGGCCATAGCCAGACAATATCATGACAAAGGGGTATCTTATGCAGAGG
AGGCAGAAGATTCCCTATATATAGTCGAATATGAATTTTTAGAATCTTTGTATGTCGATGAGCCTGATGAAGAAGCAATC
ATGGAATGTTTTGACTTTCTCAGAGATAAAATGATGTATGCTGATCTTGAAGATTTTGCCTTAGATGTGGCAAAATATTA
TCATGAAAGAGAGAATTTCGAGAAGGCTGCTGCATATTTTTTAAAGGTGGAAGAAACAAGACAGCAGATTCAAGGAGGTG
TGAAGTTGTATGAAATTGAAGTATAA

Domains

Predicted by InterproScan.

response regulator aspartate phosphatase H, N terminal (RapH_N)

(5-65)

Tetratricopeptide repeat (TPR_12)

(189-253)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rapF Bacillus subtilis subsp. subtilis str. 168

78.478

100

0.785
  rapC Bacillus subtilis subsp. subtilis str. 168

57.105

99.738

0.57

Multiple sequence alignment