Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   GRT08_RS12265 Genome accession   NZ_CP047234
Coordinates   2520195..2520962 (-) Length   255 a.a.
NCBI ID   WP_071391600.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain FJ4     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 2515195..2525962
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GRT08_RS12245 (GRT08_12075) - 2515397..2516209 (-) 813 WP_003152991.1 pyruvate, water dikinase regulatory protein -
  GRT08_RS12250 (GRT08_12080) ccpN 2516235..2516873 (-) 639 WP_003152989.1 transcriptional regulator CcpN -
  GRT08_RS12255 (GRT08_12085) glyS 2516974..2519013 (-) 2040 WP_071391601.1 glycine--tRNA ligase subunit beta -
  GRT08_RS12260 (GRT08_12090) glyQ 2519006..2519893 (-) 888 WP_003152984.1 glycine--tRNA ligase subunit alpha -
  GRT08_RS12265 (GRT08_12095) recO 2520195..2520962 (-) 768 WP_071391600.1 DNA repair protein RecO Machinery gene
  GRT08_RS12270 (GRT08_12100) - 2520999..2521142 (-) 144 WP_003152980.1 YqzL family protein -
  GRT08_RS12275 (GRT08_12105) era 2521298..2522203 (-) 906 WP_007408285.1 GTPase Era -
  GRT08_RS12280 (GRT08_12110) - 2522184..2522594 (-) 411 WP_003152976.1 cytidine deaminase -
  GRT08_RS12285 (GRT08_12115) - 2522718..2523092 (-) 375 WP_003152974.1 diacylglycerol kinase family protein -
  GRT08_RS12290 (GRT08_12120) ybeY 2523073..2523546 (-) 474 WP_003152973.1 rRNA maturation RNase YbeY -
  GRT08_RS12295 (GRT08_12125) - 2523547..2525679 (-) 2133 WP_015387990.1 HD family phosphohydrolase -

Sequence

Protein


Download         Length: 255 a.a.        Molecular weight: 29273.87 Da        Isoelectric Point: 8.3139

>NTDB_id=411693 GRT08_RS12265 WP_071391600.1 2520195..2520962(-) (recO) [Bacillus amyloliquefaciens strain FJ4]
MLTKCEGIVLRTNDYGETNKIVTLLTREHGKIGVMARGARKSASRLSAVSQPFLYGSFLMQRTSGLGTLQQGEMILSMRT
IREDLFLTAYAAFIAELTDKGTEEKKPNPYLFELILESFKRLNDGTDPDVITFIVQMKMLGVMGLYPELNHCVHCKSGEG
TFHFSIRDNGFICHRCFEKDPYRVPISPQTARLLRLFYYFDIGRLGSVSLKQETKNEIKHVIDLYYEEYSGLYLKSKRFL
DQMESMKNLLDENKS

Nucleotide


Download         Length: 768 bp        

>NTDB_id=411693 GRT08_RS12265 WP_071391600.1 2520195..2520962(-) (recO) [Bacillus amyloliquefaciens strain FJ4]
ATGCTGACTAAATGTGAAGGAATCGTGCTCCGTACGAATGACTACGGGGAGACGAATAAAATCGTGACATTGCTGACGAG
GGAACACGGCAAAATCGGCGTCATGGCAAGGGGCGCCAGGAAGTCAGCCAGCCGGCTGTCAGCAGTCAGCCAGCCGTTTT
TGTACGGGTCATTTTTAATGCAAAGAACATCCGGACTCGGCACGCTCCAGCAGGGTGAAATGATTCTCAGCATGAGAACG
ATCAGAGAAGATTTATTTTTGACGGCGTATGCCGCTTTTATCGCTGAACTCACTGACAAAGGAACGGAAGAGAAGAAACC
GAACCCCTATTTATTCGAATTGATTCTCGAATCCTTTAAGAGGCTGAATGACGGGACTGACCCGGACGTGATCACCTTCA
TCGTGCAGATGAAAATGCTCGGCGTCATGGGGCTGTATCCGGAGCTGAATCATTGTGTCCACTGCAAAAGCGGGGAAGGG
ACGTTTCATTTTTCGATAAGAGATAACGGTTTTATCTGCCACCGCTGCTTTGAAAAGGATCCGTACAGAGTTCCGATTTC
GCCGCAAACGGCAAGGCTGCTAAGGCTTTTTTATTACTTTGATATCGGAAGGCTCGGCAGCGTCTCGTTAAAACAGGAAA
CGAAAAACGAAATTAAACATGTCATTGATCTGTATTACGAGGAATATTCAGGACTTTATTTAAAGTCAAAACGGTTTTTA
GACCAGATGGAAAGCATGAAGAACCTTTTGGATGAAAACAAAAGTTGA

Domains

Predicted by InterproScan.

Recombination protein O C terminal (RecO_C)

(82-237)

Recombination protein O N terminal (RecO_N)

(1-75)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

89.02

100

0.89

Multiple sequence alignment