Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA   Type   Machinery gene
Locus tag   GRT08_RS08270 Genome accession   NZ_CP047234
Coordinates   1621438..1622337 (+) Length   299 a.a.
NCBI ID   WP_071391836.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain FJ4     
Function   ssDNA binding; loading RecA onto ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1616438..1627337
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GRT08_RS08250 (GRT08_08135) - 1617129..1618832 (+) 1704 WP_377803135.1 glycosyl transferase -
  GRT08_RS08255 (GRT08_08140) - 1618829..1619110 (+) 282 WP_007409769.1 FlhB-like flagellar biosynthesis protein -
  GRT08_RS08260 (GRT08_08145) sucC 1619285..1620442 (+) 1158 WP_003154283.1 ADP-forming succinate--CoA ligase subunit beta -
  GRT08_RS08265 (GRT08_08150) sucD 1620471..1621373 (+) 903 WP_003154281.1 succinate--CoA ligase subunit alpha -
  GRT08_RS08270 (GRT08_08155) dprA 1621438..1622337 (+) 900 WP_071391836.1 DNA-processing protein DprA Machinery gene
  GRT08_RS08275 (GRT08_08160) topA 1622519..1624594 (+) 2076 WP_031378741.1 type I DNA topoisomerase -
  GRT08_RS08280 (GRT08_08165) trmFO 1624659..1625966 (+) 1308 WP_012117550.1 FADH(2)-oxidizing methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))- methyltransferase TrmFO -
  GRT08_RS08285 (GRT08_08170) xerC 1626036..1626953 (+) 918 WP_007409774.1 tyrosine recombinase XerC -

Sequence

Protein


Download         Length: 299 a.a.        Molecular weight: 32898.98 Da        Isoelectric Point: 7.5073

>NTDB_id=411677 GRT08_RS08270 WP_071391836.1 1621438..1622337(+) (dprA) [Bacillus amyloliquefaciens strain FJ4]
MDQASRCLMVCSINQIISPSLLLKWWKADHSLSFLSDPHPLTVLSEGKTAPEEIFREIERKDPELDEVLSDYRREGITVI
PISSTRYPTWLKAIYDPPAVLYAKGNTLLLEKGRKIGIVGTRKPTEDGIKAAGHLSAELSKKGWVIVSGLASGIDGLSHK
ASIRAKGLTIGVIAGGFHHIYPRENLLLAEYMAEHHLLLSEHPPETKPKKWHFPMRNRIISGLSEGIVVVQGKEKSGSLI
TAYQALDQGREVFAVPGSIFNPYSGGPIKLIQEGAKAVLCAEDIDGELTARCVQYTEPF

Nucleotide


Download         Length: 900 bp        

>NTDB_id=411677 GRT08_RS08270 WP_071391836.1 1621438..1622337(+) (dprA) [Bacillus amyloliquefaciens strain FJ4]
TTGGATCAAGCATCGCGCTGTTTAATGGTCTGCAGTATTAATCAAATCATTTCCCCGTCTCTTCTATTAAAATGGTGGAA
AGCTGATCACTCTCTGTCTTTTTTATCGGATCCGCATCCATTAACTGTTTTATCAGAAGGGAAAACAGCCCCGGAAGAAA
TTTTTCGGGAAATAGAGCGCAAGGATCCGGAACTTGATGAAGTTCTGTCCGATTACCGCCGCGAAGGCATTACTGTCATT
CCGATTTCATCAACCCGCTACCCAACATGGCTTAAAGCGATTTATGATCCGCCGGCTGTCTTGTATGCAAAAGGAAACAC
GCTGCTTCTTGAAAAAGGCAGAAAAATCGGGATTGTAGGAACGCGGAAACCGACGGAAGACGGAATAAAAGCGGCTGGGC
ATCTTTCCGCCGAACTCTCAAAAAAAGGCTGGGTCATTGTAAGCGGGCTTGCATCCGGTATAGACGGATTGTCTCATAAG
GCGAGCATCAGGGCAAAAGGGCTTACGATCGGCGTGATAGCCGGCGGATTCCATCACATCTATCCCCGGGAAAATCTCCT
GTTAGCAGAATACATGGCTGAACACCATCTCCTACTCTCAGAACATCCTCCTGAAACAAAGCCGAAAAAATGGCATTTTC
CGATGAGAAACCGCATAATCAGCGGACTAAGTGAAGGAATTGTGGTCGTGCAGGGAAAAGAAAAAAGCGGTTCATTAATC
ACAGCTTACCAGGCCCTCGATCAAGGCAGAGAGGTATTTGCCGTTCCGGGTTCCATATTTAATCCATATTCCGGAGGACC
TATAAAACTCATTCAAGAAGGGGCGAAAGCTGTATTATGCGCAGAGGATATTGACGGAGAGCTGACCGCCCGATGCGTTC
AGTATACGGAACCCTTTTGA

Domains

Predicted by InterproScan.

DNA recombination-mediator protein A (DNA_processg_A)

(73-278)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Bacillus subtilis subsp. subtilis str. 168

69.9

100

0.699
  dprA Lactococcus lactis subsp. cremoris KW2

41.288

88.294

0.365

Multiple sequence alignment