Detailed information NTG0411270

Bioinformatically predicted

Overview

• Name: amiE
• Type: Regulator
• Locus tag: H4J01_RS01165
• Genome accession: NZ_CP059840
• Coordinates: 228747..229730 (+)
• Length: 327 a.a.
• NCBI ID: WP_001196477.1
• Uniprot ID: A0A0H2VBW7
• Organism: Escherichia coli strain E2348/69
• Function: internalize XIP (predicted from homology) Competence regulation

Genomic Context

Location: 223747..234730

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
H4J01_RS01150	dppA	224890..226497 (+)	1608	WP_001222893.1	dipeptide ABC transporter substrate-binding protein DppA	-
H4J01_RS01155	dppB	226805..227824 (+)	1020	WP_000938855.1	dipeptide ABC transporter permease DppB	-
H4J01_RS01160	dppC	227834..228736 (+)	903	WP_000084677.1	dipeptide ABC transporter permease DppC	-
H4J01_RS01165	amiE	228747..229730 (+)	984	WP_001196477.1	dipeptide ABC transporter ATP-binding protein	Regulator
H4J01_RS01170	dppF	229727..230740 (+)	1014	WP_000103582.1	dipeptide ABC transporter ATP-binding subunit DppF	-
H4J01_RS01175	-	230965..231288 (+)	324	WP_000563102.1	helix-turn-helix domain-containing protein	-
H4J01_RS01180	-	231273..231584 (+)	312	WP_001346664.1	HipA N-terminal domain-containing protein	-
H4J01_RS01185	-	231584..232591 (+)	1008	WP_001262465.1	type II toxin-antitoxin system HipA family toxin	-
H4J01_RS01190	yhjV	232608..233879 (-)	1272	WP_001339917.1	aromatic amino acid transport family protein	-
H4J01_RS01195	-	234355..234462 (+)	108	WP_000170747.1	type I toxin-antitoxin system toxin Ldr family protein	-

Sequence

Protein

• Length: 327 a.a.
• Molecular weight: 35801.38 Da
• Isoelectric Point: 6.5813

>NTDB_id=411270 H4J01_RS01165 WP_001196477.1 228747..229730(+) (amiE) [Escherichia coli strain E2348/69]
MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGSGKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISE
KERRNLVGAEVAMIFQDPMTSLNPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNLVGIPDPASRLDVYPHQLSGGMSQ
RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVAEAAHKIIVMYAGQVVETGDAHA
IFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPGKYDRPNGCLLNPRCPYATDKCRAEEPALNMLADGRQSKCHYPLD
DAGRPTL

Nucleotide

• Length: 984 bp

>NTDB_id=411270 H4J01_RS01165 WP_001196477.1 228747..229730(+) (amiE) [Escherichia coli strain E2348/69]
ATGGCGTTATTAAATGTAGATAAATTATCGGTGCATTTCGGCGACGAAAGCGCACCGTTCCGCGCCGTAGATCGCATCAG
CTACAGCGTAAAACAGGGCGAAGTGGTCGGGATTGTGGGTGAGTCCGGCTCTGGTAAATCGGTCAGTTCGCTGGCAATTA
TGGGGCTGATTGATTATCCGGGCCGCGTAATGGCGGAAAAGCTGGAGTTTAACGGTCAGGATTTGCAGCGTATCTCAGAA
AAAGAGCGCCGCAACCTGGTGGGTGCCGAAGTGGCAATGATCTTCCAGGACCCGATGACCAGCCTTAACCCGTGCTACAC
CGTGGGTTTCCAGATTATGGAAGCGATTAAGGTGCATCAGGGCGGCAACAAAAGTACCCGCCGTCAGCGAGCGATTGACC
TGCTGAATCTGGTCGGTATTCCCGATCCGGCATCGCGTCTGGATGTTTACCCGCATCAGCTTTCCGGCGGCATGAGCCAG
CGCGTGATGATCGCCATGGCGATTGCCTGTCGGCCAAAACTGCTGATTGCCGATGAACCGACCACTGCGCTGGACGTGAC
CATTCAGGCGCAAATTATCGAACTGCTGCTGGAGCTACAGCAGAAAGAGAACATGGCGCTGGTGTTGATCACTCATGACC
TGGCGCTGGTGGCGGAAGCGGCACATAAAATTATCGTGATGTATGCAGGCCAGGTAGTAGAAACGGGCGATGCGCACGCT
ATCTTCCATGCGCCGCGTCACCCGTATACTCAGGCATTGCTGCGTGCGCTGCCGGAATTTGCTCAGGACAAAGAACGTCT
GGCGTCGTTGCCAGGTGTCGTTCCCGGCAAGTACGACCGCCCGAACGGCTGCCTGCTTAACCCGCGCTGCCCCTACGCCA
CTGATAAATGCCGTGCTGAAGAACCGGCGCTGAATATGCTCGCTGACGGGCGTCAGTCCAAATGCCATTACCCACTTGAT
GATGCCGGGAGGCCGACACTATGA

Domains

Predicted by InterProScan.

• ABC transporter (ABC_tran) (24-181)

• Oligopeptide/dipeptide transporter, C-terminal region (oligo_HPY) (233-297)

3D structure

• AlphaFold DB: A0A0H2VBW7

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
amiE	Streptococcus salivarius strain HSISS4	43.302	98.165	0.425
amiE	Streptococcus thermophilus LMG 18311	42.991	98.165	0.422
amiE	Streptococcus thermophilus LMD-9	42.991	98.165	0.422
oppD	Streptococcus mutans UA159	41.379	97.554	0.404