Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   F9B76_RS00575 Genome accession   NZ_CP047165
Coordinates   153629..154987 (+) Length   452 a.a.
NCBI ID   WP_159990337.1    Uniprot ID   -
Organism   Pelistega ratti strain NLN63     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 148629..159987
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F9B76_RS00555 - 149319..150524 (+) 1206 WP_159990333.1 NnrS family protein -
  F9B76_RS00560 - 150627..151481 (-) 855 WP_159990334.1 squalene/phytoene synthase family protein -
  F9B76_RS00565 - 151644..152336 (-) 693 WP_159990335.1 amino acid racemase -
  F9B76_RS00570 alr 152470..153612 (+) 1143 WP_159990336.1 alanine racemase -
  F9B76_RS00575 radA 153629..154987 (+) 1359 WP_159990337.1 DNA repair protein RadA Machinery gene
  F9B76_RS00580 - 155080..155805 (+) 726 WP_159990338.1 3-deoxy-D-manno-octulosonic acid kinase -
  F9B76_RS00585 - 155982..157085 (+) 1104 WP_159990339.1 polyamine ABC transporter substrate-binding protein -
  F9B76_RS00590 potA 157282..158406 (+) 1125 WP_159990340.1 polyamine ABC transporter ATP-binding protein -
  F9B76_RS00595 - 158406..159314 (+) 909 WP_159992025.1 ABC transporter permease subunit -

Sequence

Protein


Download         Length: 452 a.a.        Molecular weight: 48859.50 Da        Isoelectric Point: 7.4870

>NTDB_id=411260 F9B76_RS00575 WP_159990337.1 153629..154987(+) (radA) [Pelistega ratti strain NLN63]
MAKAKTSFVCQECGGVSTKWQGRCPHCQQWNTMVEFVEKSTSAHQNRFAHLAQTAPIKILSEVVATEVPRILTGLSEFDR
VLGGGLVPGGVVLIGGDPGIGKSTLLIQALSYLSIQHDVLYVTGEESAEQVALRANRLELETKNVKLLAEIQLEAIQTAL
QSERPKIAVIDSIQTLFSGELTAAPGSVSQVRECAAQLTRIAKQNGIAIVMIGHVTKDGTLAGPRVLEHIVDTVLYFEGE
SDSTHRLIRAFKNRFGAVNELGVFAMTDKGLKGINNPSAIFLSQHNEDIPGSCVMATQEGTRPLLVEIQALVDNAHVPNP
RRLSLGIDGGRLAMLLAVLNRHVGIVTFDQDVFVNAVGGVRITETAADLPVLLSIISSLRDKPLPKGLFAFGEIGLAGEI
RPASRGQERLKEAAKLGFKIAMIPKHNMPKQTIDGLTLWPVSRLDDVVNYLR

Nucleotide


Download         Length: 1359 bp        

>NTDB_id=411260 F9B76_RS00575 WP_159990337.1 153629..154987(+) (radA) [Pelistega ratti strain NLN63]
ATGGCAAAGGCAAAAACCAGTTTTGTATGTCAAGAATGTGGTGGTGTAAGTACCAAATGGCAAGGTCGTTGTCCCCATTG
TCAGCAGTGGAATACGATGGTTGAGTTTGTTGAGAAAAGTACATCCGCTCATCAAAATCGTTTTGCTCATTTGGCACAAA
CAGCTCCCATTAAAATCCTTTCAGAGGTCGTTGCGACAGAAGTTCCTCGTATTCTAACGGGTTTATCAGAATTTGACCGT
GTTTTAGGGGGTGGTTTAGTACCCGGTGGTGTTGTTCTCATTGGGGGTGATCCGGGGATAGGTAAATCAACTCTCTTAAT
TCAAGCATTATCCTATCTATCTATTCAGCATGATGTTTTATATGTAACAGGTGAAGAGTCGGCAGAGCAAGTGGCATTAA
GAGCTAATCGATTAGAGTTAGAAACAAAAAATGTTAAATTATTAGCTGAAATTCAATTAGAAGCGATTCAAACTGCCTTA
CAATCAGAACGTCCTAAGATAGCAGTTATTGACTCTATTCAAACGCTTTTTTCAGGTGAGCTGACGGCGGCTCCTGGATC
TGTTTCTCAAGTACGAGAATGTGCTGCTCAACTGACACGTATTGCAAAACAAAATGGTATTGCTATTGTGATGATTGGAC
ACGTTACCAAAGATGGAACATTGGCTGGTCCAAGAGTGCTAGAGCATATTGTTGATACGGTTTTATATTTTGAAGGAGAA
AGTGATTCAACACATCGCTTAATCCGTGCTTTTAAAAATCGTTTTGGTGCGGTGAATGAGTTAGGTGTTTTTGCGATGAC
TGATAAGGGGCTTAAAGGAATTAATAACCCTTCAGCCATTTTTCTTTCGCAACATAATGAGGATATTCCCGGATCCTGTG
TGATGGCAACACAGGAGGGAACACGCCCTTTATTAGTGGAAATACAAGCATTGGTTGATAATGCGCATGTCCCTAATCCC
AGAAGATTGAGTTTGGGGATTGACGGAGGACGTTTAGCCATGCTTTTAGCTGTCTTAAACCGCCATGTAGGTATTGTTAC
TTTTGATCAAGATGTTTTTGTGAATGCTGTTGGCGGTGTCCGTATTACAGAAACTGCCGCTGATTTGCCTGTATTATTAT
CTATTATTTCTTCTTTGCGTGATAAACCTCTTCCCAAAGGACTTTTTGCTTTTGGTGAAATTGGTTTGGCAGGAGAGATT
CGACCAGCCTCAAGAGGGCAAGAGCGTTTAAAAGAAGCGGCAAAATTAGGATTTAAAATCGCTATGATTCCTAAGCATAA
TATGCCTAAACAAACGATTGATGGTTTAACACTATGGCCTGTTTCTCGCTTAGATGATGTCGTTAATTATTTACGATAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(75-219)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

50.794

97.566

0.496
  radA Streptococcus pneumoniae Rx1

48.519

97.124

0.471
  radA Streptococcus pneumoniae D39

48.519

97.124

0.471
  radA Streptococcus pneumoniae R6

48.519

97.124

0.471
  radA Streptococcus pneumoniae TIGR4

48.519

97.124

0.471
  radA Streptococcus mitis NCTC 12261

48.951

94.912

0.465
  radA Streptococcus mitis SK321

48.718

94.912

0.462

Multiple sequence alignment