Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   GRF56_RS21930 Genome accession   NZ_CP047155
Coordinates   4746370..4747809 (-) Length   479 a.a.
NCBI ID   WP_111899810.1    Uniprot ID   -
Organism   Aeromonas veronii strain AVNIH1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4741370..4752809
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GRF56_RS21900 (GRF56_21900) dagF 4741638..4742378 (+) 741 WP_043850965.1 2-dehydro-3-deoxy-phosphogluconate aldolase -
  GRF56_RS21905 (GRF56_21905) - 4742486..4744390 (+) 1905 WP_159144467.1 BglG family transcription antiterminator -
  GRF56_RS21910 (GRF56_21910) npr 4744408..4744680 (-) 273 WP_005336318.1 PTS phosphocarrier protein NPr -
  GRF56_RS21915 (GRF56_21915) rapZ 4744701..4745567 (-) 867 WP_111899812.1 RNase adapter RapZ -
  GRF56_RS21920 (GRF56_21920) ptsN 4745613..4746059 (-) 447 WP_111899811.1 PTS IIA-like nitrogen regulatory protein PtsN -
  GRF56_RS21925 (GRF56_21925) hpf 4746062..4746349 (-) 288 WP_005336323.1 ribosome hibernation promoting factor -
  GRF56_RS21930 (GRF56_21930) rpoN/rpoN1 4746370..4747809 (-) 1440 WP_111899810.1 RNA polymerase factor sigma-54 Machinery gene
  GRF56_RS21935 (GRF56_21935) lptB 4747875..4748600 (-) 726 WP_005336333.1 LPS export ABC transporter ATP-binding protein -
  GRF56_RS21940 (GRF56_21940) lptA 4748604..4749131 (-) 528 WP_040068817.1 lipopolysaccharide transport periplasmic protein LptA -
  GRF56_RS21945 (GRF56_21945) lptC 4749118..4749678 (-) 561 WP_111899809.1 LPS export ABC transporter periplasmic protein LptC -
  GRF56_RS21950 (GRF56_21950) kdsC 4749675..4750229 (-) 555 WP_101530526.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  GRF56_RS21955 (GRF56_21955) - 4750229..4751224 (-) 996 WP_005336342.1 KpsF/GutQ family sugar-phosphate isomerase -
  GRF56_RS21960 (GRF56_21960) mlaF 4751432..4752232 (+) 801 WP_111899808.1 phospholipid ABC transporter ATP-binding protein MlaF -

Sequence

Protein


Download         Length: 479 a.a.        Molecular weight: 53686.67 Da        Isoelectric Point: 4.3442

>NTDB_id=411101 GRF56_RS21930 WP_111899810.1 4746370..4747809(-) (rpoN/rpoN1) [Aeromonas veronii strain AVNIH1]
MKPTLQLRLGQSLTMTPQLQQAIRLLQLSTLELQQEIQQALENNPLLEQEESEAQETSPEAPVDASQLDSSDAMAQEQMP
DELPVDTTWDEVYSAGTAQSAGPIHDGEDSVYQGETTENLQDYLLWQMRLTPFSDVDAAIALAIIDAIDESGYLTVALED
IQAAVTSDDVEVELDEVEAVLKRVQHFDPVGIAARSVQECLLIQLDQYDPQTPWLNEAKEVILEHMELLGNRDYRTLARK
TKLKESDLKEVLDLILLLEPRPGNGIIQSDSQYVIPDVSVVKKGSKWVVELNPDSAPRIRVNETYAAMARNARSSTDTQF
IRSHLQEAKWFIKSLESRNDTLLKVASCIVEQQQGFFEYGEEAMKPMVLNDIAEAVEMHESTISRVTTQKYMHTPRGIFE
LKFFFSSHVNTEGGGECSSTAIRALIKKLVTAENPAKPLSDSKIADLLAEQGIMVARRTIAKYRESLFIPPSNQRKRLV

Nucleotide


Download         Length: 1440 bp        

>NTDB_id=411101 GRF56_RS21930 WP_111899810.1 4746370..4747809(-) (rpoN/rpoN1) [Aeromonas veronii strain AVNIH1]
ATGAAGCCGACATTACAGTTGCGTCTCGGTCAGTCCCTGACCATGACGCCGCAATTACAACAAGCGATTCGTCTGCTCCA
GCTCTCCACTCTGGAACTTCAGCAGGAAATTCAGCAAGCGCTCGAAAACAACCCTCTGCTGGAGCAGGAAGAGAGCGAGG
CGCAAGAGACCAGTCCGGAAGCGCCGGTCGATGCCAGCCAGCTGGATTCCAGCGATGCCATGGCTCAGGAGCAGATGCCG
GACGAGTTACCGGTGGATACCACCTGGGACGAGGTTTACTCTGCCGGCACCGCCCAGAGCGCGGGCCCCATCCACGATGG
GGAAGATAGCGTCTATCAGGGTGAAACTACCGAAAACCTGCAGGATTATCTGCTGTGGCAGATGCGGCTCACACCTTTCA
GCGATGTGGATGCCGCCATCGCGCTCGCCATCATCGATGCCATCGACGAATCCGGTTATCTGACCGTTGCGCTGGAGGAT
ATCCAGGCTGCCGTCACCAGCGATGACGTAGAGGTGGAACTCGATGAGGTCGAGGCGGTACTCAAGCGGGTGCAGCACTT
CGATCCGGTCGGTATTGCTGCCCGTTCTGTGCAGGAGTGTTTGCTGATCCAGCTGGATCAGTATGACCCGCAGACCCCCT
GGCTCAACGAGGCCAAAGAGGTGATCCTTGAGCACATGGAACTGCTCGGCAACCGGGATTACCGTACCCTCGCCCGCAAG
ACAAAACTCAAAGAAAGCGACCTCAAGGAGGTGCTGGATCTGATCCTGCTGCTGGAGCCGCGTCCCGGCAACGGCATCAT
CCAGAGCGACTCCCAGTACGTGATCCCCGACGTCTCCGTGGTCAAGAAGGGCAGCAAGTGGGTGGTCGAGCTCAATCCCG
ACTCCGCCCCCCGCATCCGGGTCAACGAGACCTATGCCGCCATGGCCCGCAACGCTCGCAGCAGCACCGACACCCAGTTT
ATCCGCTCCCATTTGCAGGAAGCCAAGTGGTTCATCAAGAGCCTGGAAAGTCGCAATGACACCCTGCTGAAAGTGGCCAG
TTGTATTGTCGAGCAGCAACAGGGCTTTTTCGAGTATGGTGAAGAGGCGATGAAGCCGATGGTGCTCAATGACATCGCCG
AAGCGGTAGAGATGCATGAGTCGACCATCTCCCGGGTGACGACCCAGAAGTACATGCATACCCCTCGTGGTATCTTCGAA
TTGAAGTTTTTTTTCTCCAGCCATGTGAACACCGAAGGAGGAGGAGAGTGCTCATCGACCGCAATTCGCGCGCTCATCAA
GAAGCTGGTCACGGCAGAGAATCCTGCCAAGCCGTTAAGTGATAGCAAGATCGCGGATTTGCTGGCCGAACAGGGTATTA
TGGTGGCGAGACGCACGATCGCTAAGTATCGCGAATCCTTGTTTATCCCACCTTCCAATCAGCGCAAACGCCTGGTTTAG

Domains

Predicted by InterProScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(319-477)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-305)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.988

100

0.491

Multiple sequence alignment