Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   GST84_RS22485 Genome accession   NZ_CP047152
Coordinates   4832902..4834272 (-) Length   456 a.a.
NCBI ID   WP_003251626.1    Uniprot ID   I7AVL1
Organism   Pseudomonas putida strain PP27     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4827902..4839272
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GST84_RS22465 (GST84_22150) - 4828264..4828461 (-) 198 WP_003251633.1 YbdD/YjiX family protein -
  GST84_RS22470 (GST84_22155) - 4828479..4830545 (-) 2067 WP_021784291.1 carbon starvation CstA family protein -
  GST84_RS22475 (GST84_22160) - 4830723..4831085 (+) 363 WP_021784292.1 PilZ domain-containing protein -
  GST84_RS22480 (GST84_22165) - 4831349..4832866 (+) 1518 WP_021784293.1 nucleobase:cation symporter-2 family protein -
  GST84_RS22485 (GST84_22170) radA 4832902..4834272 (-) 1371 WP_003251626.1 DNA repair protein RadA Machinery gene
  GST84_RS22490 (GST84_22175) mscL 4834449..4834868 (+) 420 WP_021784294.1 large-conductance mechanosensitive channel protein MscL -
  GST84_RS22495 (GST84_22180) - 4834928..4835704 (-) 777 WP_028698125.1 ferredoxin--NADP reductase -
  GST84_RS22500 (GST84_22185) - 4835920..4836627 (+) 708 WP_021784296.1 autoinducer binding domain-containing protein -
  GST84_RS22505 (GST84_22190) - 4836724..4837848 (+) 1125 WP_021784297.1 methyltransferase -
  GST84_RS22510 (GST84_22195) - 4837934..4838089 (-) 156 WP_016713451.1 DUF2474 domain-containing protein -
  GST84_RS22515 (GST84_22200) cydB 4838099..4839106 (-) 1008 WP_003251612.1 cytochrome d ubiquinol oxidase subunit II -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 48741.18 Da        Isoelectric Point: 6.8985

>NTDB_id=411065 GST84_RS22485 WP_003251626.1 4832902..4834272(-) (radA) [Pseudomonas putida strain PP27]
MAKAKRLYGCTECGATFPKWAGQCGECGAWNTLVETMIESGGAAAPSSGRAGWTGQQAQIKTLAEVSVEEIPRFTTSSTE
LDRVLGGGLVDGSVVLIGGDPGIGKSTILLQTLCNIAVGMPALYVTGEESQQQVAMRSRRLGLPQDQLKVMTETCIETII
ATARVEKPRVMVIDSIQTIFTEQLQSAPGGVAQVRESTALLVRYAKQSGTAIFLVGHVTKEGSLAGPRVLEHMVDTVLYF
EGESDGRLRLLRAVKNRFGAVNELGVFGMTDRGLKEVSNPSAIFLNRTQEEVPGSVVMATWEGTRPMLVEVQALVDDSHL
ANPRRVTLGLDQNRLAMLLAVLHRHGGIPTHDQDVFLNVVGGVKVLETASDLALLAAVMSSLRNRPLAHGLLVFGEIGLS
GEVRPVPSGQERLKEAAKHGFKRAIVPKGNAPKEAPAGLQVIAVTRLEQALDALFE

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=411065 GST84_RS22485 WP_003251626.1 4832902..4834272(-) (radA) [Pseudomonas putida strain PP27]
ATGGCCAAGGCTAAGCGCTTGTATGGCTGCACCGAATGCGGCGCGACTTTCCCCAAATGGGCTGGCCAGTGCGGCGAATG
CGGGGCCTGGAACACCCTGGTCGAAACCATGATCGAAAGCGGCGGCGCTGCTGCACCCAGCAGTGGCCGCGCTGGCTGGA
CGGGACAGCAGGCGCAGATCAAGACCCTGGCCGAAGTCAGTGTTGAGGAAATCCCGCGTTTCACGACCAGCAGCACCGAA
CTGGACCGCGTGCTGGGCGGCGGCCTGGTGGATGGCTCGGTGGTGCTGATCGGCGGTGACCCCGGCATCGGCAAATCGAC
CATTTTGCTACAGACCTTGTGCAACATCGCCGTGGGCATGCCGGCACTGTATGTCACAGGGGAGGAGTCGCAGCAGCAGG
TGGCCATGCGCTCCCGGCGGCTGGGGCTACCTCAGGACCAGCTCAAGGTGATGACCGAAACCTGCATCGAGACGATCATT
GCCACGGCGCGGGTCGAGAAGCCGAGGGTAATGGTGATCGACTCGATCCAGACCATTTTCACCGAGCAGTTGCAATCGGC
CCCGGGCGGCGTGGCTCAGGTGCGCGAAAGCACAGCGTTGCTGGTGCGCTACGCCAAGCAAAGCGGTACGGCAATCTTTC
TGGTCGGCCACGTGACCAAGGAAGGCTCGCTGGCTGGCCCGAGGGTGCTGGAGCACATGGTCGACACTGTGCTGTATTTC
GAAGGTGAGTCCGATGGCCGTCTGCGCTTGCTGCGGGCGGTCAAGAACCGCTTTGGCGCGGTCAACGAACTGGGCGTGTT
CGGCATGACCGATCGTGGCCTCAAGGAGGTATCGAACCCGTCGGCGATCTTCCTCAACCGTACTCAGGAAGAGGTGCCGG
GTAGCGTGGTGATGGCAACCTGGGAGGGGACCCGGCCAATGCTGGTCGAGGTGCAGGCGCTGGTCGACGATAGCCATCTG
GCCAACCCGCGGCGGGTGACCTTGGGCCTGGATCAGAACCGCCTGGCCATGTTGTTGGCGGTACTCCACCGTCATGGCGG
CATCCCGACCCATGACCAGGATGTGTTTCTCAACGTGGTCGGCGGGGTGAAGGTGCTGGAAACGGCTTCGGACCTGGCGC
TGCTGGCGGCGGTGATGTCCAGCCTGCGTAACCGGCCACTGGCCCATGGTTTGCTGGTGTTTGGCGAGATTGGCTTGTCG
GGCGAGGTACGGCCGGTGCCCAGTGGCCAGGAGCGCTTGAAGGAAGCGGCCAAGCACGGCTTCAAGCGTGCCATCGTACC
CAAGGGCAATGCGCCGAAAGAAGCGCCGGCGGGGTTGCAGGTGATTGCCGTTACCCGCCTGGAACAGGCGCTGGATGCAT
TGTTCGAATAG

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(355-430)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(73-222)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB I7AVL1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

48.684

100

0.487
  radA Streptococcus pneumoniae Rx1

46.087

100

0.465
  radA Streptococcus pneumoniae D39

46.087

100

0.465
  radA Streptococcus pneumoniae TIGR4

46.087

100

0.465
  radA Streptococcus pneumoniae R6

46.087

100

0.465
  radA Streptococcus mitis NCTC 12261

45.87

100

0.463
  radA Streptococcus mitis SK321

46.053

100

0.461

Multiple sequence alignment