Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   GST86_RS24495 Genome accession   NZ_CP047148
Coordinates   5264825..5266195 (-) Length   456 a.a.
NCBI ID   WP_003251626.1    Uniprot ID   I7AVL1
Organism   Pseudomonas putida strain PP2323     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 5259825..5271195
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GST86_RS24475 (GST86_24170) - 5260187..5260384 (-) 198 WP_003251633.1 YbdD/YjiX family protein -
  GST86_RS24480 (GST86_24175) - 5260402..5262468 (-) 2067 WP_004577217.1 carbon starvation CstA family protein -
  GST86_RS24485 (GST86_24180) - 5262646..5263008 (+) 363 WP_029615590.1 PilZ domain-containing protein -
  GST86_RS24490 (GST86_24185) - 5263272..5264789 (+) 1518 WP_010955302.1 nucleobase:cation symporter-2 family protein -
  GST86_RS24495 (GST86_24190) radA 5264825..5266195 (-) 1371 WP_003251626.1 DNA repair protein RadA Machinery gene
  GST86_RS24500 (GST86_24195) mscL 5266372..5266791 (+) 420 WP_010955303.1 large-conductance mechanosensitive channel protein MscL -
  GST86_RS24505 (GST86_24200) - 5266915..5267691 (-) 777 WP_010955304.1 ferredoxin--NADP reductase -
  GST86_RS24510 (GST86_24205) - 5267907..5268614 (+) 708 WP_049587090.1 autoinducer binding domain-containing protein -
  GST86_RS24515 (GST86_24210) - 5268711..5269835 (+) 1125 WP_010955306.1 methyltransferase -
  GST86_RS24520 (GST86_24215) - 5269941..5270096 (-) 156 WP_010955307.1 DUF2474 domain-containing protein -
  GST86_RS24525 (GST86_24220) cydB 5270106..5271113 (-) 1008 WP_010955308.1 cytochrome d ubiquinol oxidase subunit II -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 48741.18 Da        Isoelectric Point: 6.8985

>NTDB_id=411018 GST86_RS24495 WP_003251626.1 5264825..5266195(-) (radA) [Pseudomonas putida strain PP2323]
MAKAKRLYGCTECGATFPKWAGQCGECGAWNTLVETMIESGGAAAPSSGRAGWTGQQAQIKTLAEVSVEEIPRFTTSSTE
LDRVLGGGLVDGSVVLIGGDPGIGKSTILLQTLCNIAVGMPALYVTGEESQQQVAMRSRRLGLPQDQLKVMTETCIETII
ATARVEKPRVMVIDSIQTIFTEQLQSAPGGVAQVRESTALLVRYAKQSGTAIFLVGHVTKEGSLAGPRVLEHMVDTVLYF
EGESDGRLRLLRAVKNRFGAVNELGVFGMTDRGLKEVSNPSAIFLNRTQEEVPGSVVMATWEGTRPMLVEVQALVDDSHL
ANPRRVTLGLDQNRLAMLLAVLHRHGGIPTHDQDVFLNVVGGVKVLETASDLALLAAVMSSLRNRPLAHGLLVFGEIGLS
GEVRPVPSGQERLKEAAKHGFKRAIVPKGNAPKEAPAGLQVIAVTRLEQALDALFE

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=411018 GST86_RS24495 WP_003251626.1 5264825..5266195(-) (radA) [Pseudomonas putida strain PP2323]
ATGGCCAAGGCCAAGCGCTTGTATGGCTGCACCGAATGCGGCGCGACCTTCCCCAAATGGGCCGGCCAGTGCGGCGAATG
CGGGGCCTGGAACACCCTGGTCGAAACCATGATCGAAAGCGGCGGCGCTGCTGCACCCAGCAGCGGTCGCGCTGGCTGGA
CAGGGCAGCAGGCGCAGATCAAGACCCTGGCCGAAGTCAGTGTCGAGGAAATCCCGCGTTTCACGACCAGCAGCACCGAA
CTGGACCGCGTGCTGGGTGGCGGCCTGGTGGATGGCTCGGTGGTGCTGATTGGCGGTGACCCCGGCATCGGCAAGTCGAC
CATTTTGCTACAGACCTTGTGCAACATTGCCGTGGGCATGCCGGCCCTGTATGTCACGGGGGAGGAGTCGCAGCAGCAGG
TGGCCATGCGCTCGCGGCGGCTGGGCCTACCCCAGGACCAACTCAAGGTGATGACCGAAACCTGCATCGAGACGATCATT
GCCACGGCGCGGGTCGAGAAGCCGCGGGTAATGGTGATCGACTCGATCCAGACCATTTTCACCGAGCAGTTGCAATCGGC
CCCGGGCGGCGTGGCCCAGGTGCGCGAAAGCACAGCGTTGCTGGTGCGCTATGCCAAGCAAAGCGGTACGGCAATCTTCC
TGGTCGGCCACGTGACCAAGGAGGGCTCGCTGGCTGGCCCAAGGGTGCTGGAGCACATGGTCGACACGGTGCTGTATTTC
GAAGGTGAGTCCGATGGCCGCCTGCGCTTGCTGCGGGCGGTCAAGAATCGTTTTGGCGCGGTCAATGAGCTGGGCGTGTT
CGGCATGACCGATCGTGGCCTCAAGGAGGTATCCAACCCGTCGGCGATCTTCCTCAACCGTACCCAGGAAGAGGTGCCGG
GTAGCGTGGTGATGGCAACCTGGGAGGGTACCCGGCCAATGCTGGTCGAGGTGCAGGCGCTGGTCGACGATAGCCATCTG
GCCAACCCGCGGCGGGTAACCTTGGGCCTGGACCAGAACCGTCTGGCCATGTTGTTGGCGGTGCTCCACCGCCATGGCGG
TATCCCGACCCATGACCAGGATGTGTTTCTCAACGTGGTCGGCGGGGTGAAGGTGCTGGAAACGGCTTCGGACCTGGCCC
TGCTGGCGGCGGTGATGTCGAGCTTGCGCAACCGGCCACTGGCCCATGGTTTGCTGGTGTTTGGCGAGATCGGATTGTCG
GGCGAGGTACGCCCGGTACCCAGTGGCCAGGAGCGCTTGAAGGAAGCGGCCAAGCACGGCTTCAAGCGTGCCATCGTGCC
CAAGGGCAATGCGCCGAAAGAAGCGCCTGCGGGGTTGCAGGTGATTGCCGTTACCCGGCTGGAACAGGCGCTGGATGCAT
TGTTCGAATAG

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(355-430)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(73-222)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB I7AVL1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

48.684

100

0.487
  radA Streptococcus pneumoniae Rx1

46.087

100

0.465
  radA Streptococcus pneumoniae D39

46.087

100

0.465
  radA Streptococcus pneumoniae TIGR4

46.087

100

0.465
  radA Streptococcus pneumoniae R6

46.087

100

0.465
  radA Streptococcus mitis NCTC 12261

45.87

100

0.463
  radA Streptococcus mitis SK321

46.053

100

0.461

Multiple sequence alignment