Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   GSR61_RS01940 Genome accession   NZ_CP047142
Coordinates   387388..388764 (+) Length   458 a.a.
NCBI ID   WP_061204435.1    Uniprot ID   A0A1C2D5J9
Organism   Lactobacillus crispatus strain C25     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 382388..393764
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GSR61_RS01910 (GSR61_01925) - 383007..383189 (+) 183 WP_005720404.1 hypothetical protein -
  GSR61_RS01915 (GSR61_01930) - 383201..383881 (+) 681 WP_005724199.1 2,3-diphosphoglycerate-dependent phosphoglycerate mutase -
  GSR61_RS01920 (GSR61_01935) - 383992..384927 (+) 936 WP_023488529.1 AAA family ATPase -
  GSR61_RS01925 (GSR61_01940) pepC 384989..386338 (-) 1350 WP_065990062.1 aminopeptidase C -
  GSR61_RS01930 (GSR61_01945) - 386406..386705 (-) 300 WP_023488531.1 hypothetical protein -
  GSR61_RS01935 (GSR61_01950) - 386837..387388 (+) 552 WP_065990060.1 dUTP diphosphatase -
  GSR61_RS01940 (GSR61_01955) radA 387388..388764 (+) 1377 WP_061204435.1 DNA repair protein RadA Machinery gene
  GSR61_RS01945 (GSR61_01960) gltX 388842..390341 (+) 1500 WP_065990058.1 glutamate--tRNA ligase -
  GSR61_RS01950 (GSR61_01965) cysS 390432..391865 (+) 1434 WP_065990056.1 cysteine--tRNA ligase -
  GSR61_RS01955 (GSR61_01970) - 391858..392301 (+) 444 WP_035443487.1 mini-ribonuclease III -
  GSR61_RS01960 (GSR61_01975) rlmB 392288..393043 (+) 756 WP_005724210.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  GSR61_RS01965 (GSR61_01980) - 393177..393722 (+) 546 WP_005720419.1 DNA-directed RNA polymerase subunit sigma -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 50228.90 Da        Isoelectric Point: 7.4214

>NTDB_id=410972 GSR61_RS01940 WP_061204435.1 387388..388764(+) (radA) [Lactobacillus crispatus strain C25]
MARVKTQYKCRSCGYISASYLGRCPNCGAWNQFEKETEEVQKRSTKATASRLIQKTGLNEPVKLDKIKAEKEERIVTKSE
ELNRVLGGGIVPGSLVLIGGDPGIGKSTLMLQIMSDLSEKYKVLYVSGEESANQIKLRADRLGVGQSNMLLYPETDMHDI
REQINDVKPDFVVIDSIQTMNEPSLDSMTGSASQVREVTSELMKIAKMDAITVFVIGHVTKEGAIAGPKILEHMVDTVLY
FEGDEHHSYRILHSVKNRFGAANEIGMFEMVNEGLREVTNPSSIFLDQRLPKSTGSAVVVSLEGTRPLLAEIQALVTPTA
FGYAKRTTSGISFNKASLLLAVLEKRGNLMLQNQDVYLTATGGIKLNEPAIDLAIAMAVASSYTDKEISPTDCFVGEVGL
TGEVRRVDKIDARVKEAAKVGFKRIFIPRHNMYSGLKDHGIEVIPVSSIPQALKLVFG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=410972 GSR61_RS01940 WP_061204435.1 387388..388764(+) (radA) [Lactobacillus crispatus strain C25]
ATGGCTAGAGTAAAAACACAATATAAGTGTCGTTCTTGCGGCTATATTTCGGCAAGTTATTTAGGCCGCTGTCCTAACTG
TGGTGCCTGGAATCAATTTGAAAAAGAAACTGAAGAAGTGCAAAAGCGTTCTACTAAGGCAACTGCTAGTCGTTTAATTC
AGAAGACTGGATTAAATGAACCGGTAAAGCTGGATAAGATTAAAGCTGAAAAAGAAGAACGAATTGTCACTAAATCTGAG
GAATTAAACCGCGTTTTAGGTGGTGGTATTGTGCCAGGCTCACTAGTTTTAATTGGTGGAGATCCCGGAATTGGTAAGTC
AACCTTGATGTTACAAATTATGAGTGACTTATCTGAAAAATATAAGGTGCTTTACGTTTCGGGAGAAGAATCAGCTAACC
AAATCAAATTGCGGGCTGACCGACTGGGTGTAGGTCAAAGTAATATGCTCTTATATCCAGAGACTGACATGCATGATATT
CGCGAACAAATCAATGATGTTAAGCCTGACTTTGTTGTGATCGACTCCATTCAAACTATGAATGAACCAAGTCTTGATTC
GATGACTGGCTCTGCCTCACAGGTTCGTGAAGTAACAAGTGAATTAATGAAAATTGCCAAGATGGATGCCATTACAGTTT
TTGTTATCGGCCACGTTACAAAAGAGGGTGCGATTGCTGGGCCTAAGATCTTGGAACATATGGTGGATACCGTTCTCTAT
TTTGAAGGGGATGAACACCATTCTTACCGAATTTTGCATTCTGTTAAAAACCGTTTTGGAGCAGCAAACGAAATTGGAAT
GTTTGAAATGGTTAATGAAGGCTTAAGAGAGGTAACTAATCCATCATCAATTTTTCTGGACCAAAGATTACCTAAGTCTA
CTGGCTCTGCTGTGGTGGTATCGCTTGAAGGAACTAGGCCACTTTTAGCAGAAATTCAGGCTTTGGTTACTCCGACAGCT
TTTGGCTATGCTAAAAGGACCACGTCAGGTATTAGTTTTAACAAAGCTTCATTGCTGCTAGCGGTGCTTGAAAAGCGGGG
AAACTTAATGTTGCAGAATCAAGATGTCTATTTGACTGCAACTGGTGGGATTAAATTGAATGAACCAGCAATTGATTTAG
CAATTGCCATGGCAGTTGCATCCAGTTATACCGATAAAGAAATTTCGCCGACAGATTGTTTTGTAGGCGAAGTGGGCTTA
ACTGGTGAAGTTCGCCGAGTTGATAAAATCGATGCACGAGTAAAAGAAGCGGCTAAAGTAGGGTTTAAGCGGATCTTTAT
TCCACGGCATAATATGTATTCTGGATTAAAAGATCATGGCATTGAGGTAATTCCCGTTTCGAGTATTCCCCAAGCTTTAA
AATTAGTTTTTGGCTAG

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(66-223)

Subunit ChlI of Mg-chelatase (ChlI)

(363-431)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-34)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1C2D5J9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

57.549

99.782

0.574
  radA Streptococcus pneumoniae Rx1

57.549

99.782

0.574
  radA Streptococcus pneumoniae D39

57.549

99.782

0.574
  radA Streptococcus pneumoniae R6

57.549

99.782

0.574
  radA Streptococcus pneumoniae TIGR4

57.549

99.782

0.574
  radA Streptococcus mitis SK321

57.549

99.782

0.574
  radA Bacillus subtilis subsp. subtilis str. 168

55.773

100

0.559

Multiple sequence alignment