Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   GE573_RS18510 Genome accession   NZ_CP047119
Coordinates   3698968..3699402 (+) Length   144 a.a.
NCBI ID   WP_007611576.1    Uniprot ID   A0A172XIP3
Organism   Bacillus velezensis strain AK-0     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3693968..3704402
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GE573_RS18505 (GE573_03695) - 3697468..3698679 (-) 1212 WP_127069693.1 cytochrome P450 -
  GE573_RS18510 (GE573_03696) nucA/comI 3698968..3699402 (+) 435 WP_007611576.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  GE573_RS18515 (GE573_03697) - 3699462..3700217 (+) 756 WP_190664293.1 YoaK family protein -
  GE573_RS18520 (GE573_03698) - 3700251..3700613 (-) 363 WP_007410383.1 hypothetical protein -
  GE573_RS18525 (GE573_03699) - 3700807..3702135 (-) 1329 WP_114354640.1 S8 family peptidase -
  GE573_RS18530 - 3702314..3702546 (+) 233 Protein_3622 hypothetical protein -
  GE573_RS18535 (GE573_03701) - 3702802..3703509 (+) 708 WP_012117604.1 poly-gamma-glutamate hydrolase family protein -
  GE573_RS18540 (GE573_03702) - 3703569..3704021 (+) 453 WP_007410380.1 OsmC family protein -
  GE573_RS18545 (GE573_03703) - 3704035..3704388 (-) 354 WP_007410379.1 multidrug efflux SMR transporter -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15528.47 Da        Isoelectric Point: 7.2418

>NTDB_id=410713 GE573_RS18510 WP_007611576.1 3698968..3699402(+) (nucA/comI) [Bacillus velezensis strain AK-0]
MNAFMKWAASLLLVISLQFGLTGAGIHSDSAARAASRYDQVLYFPLSKYPETGNHIKDAISAGHSEICTIDRGGAENRRK
ESLKGIPTKPGFDRDEWPMAVCTEGGAGADIRYVTPSDNRGAGSWVGNQMSGYSDGTRVLFIVQ

Nucleotide


Download         Length: 435 bp        

>NTDB_id=410713 GE573_RS18510 WP_007611576.1 3698968..3699402(+) (nucA/comI) [Bacillus velezensis strain AK-0]
ATGAATGCGTTTATGAAATGGGCGGCAAGTCTGCTTTTGGTGATCTCTCTTCAGTTCGGCCTTACGGGCGCCGGCATCCA
TTCGGACAGTGCTGCTCGTGCCGCATCCCGATACGATCAGGTGCTGTATTTCCCGCTGTCAAAATATCCGGAGACGGGAA
ATCATATAAAAGACGCCATTTCGGCAGGTCATTCTGAGATTTGTACAATTGATCGGGGCGGAGCGGAGAATAGAAGGAAA
GAATCATTGAAAGGAATTCCGACAAAGCCCGGGTTTGACCGTGATGAATGGCCCATGGCAGTCTGCACAGAAGGCGGAGC
GGGCGCTGATATCAGATATGTAACCCCGTCGGATAACCGCGGGGCTGGTTCATGGGTTGGAAATCAAATGAGCGGATATT
CCGACGGCACGAGAGTATTATTTATCGTTCAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-138)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A172XIP3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

56.452

86.111

0.486

Multiple sequence alignment