Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   GRB21_RS24830 Genome accession   NZ_CP047073
Coordinates   5243204..5244571 (-) Length   455 a.a.
NCBI ID   WP_005770187.1    Uniprot ID   Q87WB3
Organism   Pseudomonas syringae pv. tomato strain delta X     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 5238204..5249571
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GRB21_RS24805 - 5238559..5239128 (+) 570 WP_011105095.1 YdcF family protein -
  GRB21_RS24810 yjiA 5239156..5240133 (-) 978 WP_005770183.1 GTPase -
  GRB21_RS24815 - 5240229..5240426 (-) 198 WP_011105096.1 YbdD/YjiX family protein -
  GRB21_RS24820 - 5240454..5242529 (-) 2076 WP_005770185.1 carbon starvation CstA family protein -
  GRB21_RS24825 - 5242815..5243183 (+) 369 WP_005770186.1 PilZ domain-containing protein -
  GRB21_RS24830 radA 5243204..5244571 (-) 1368 WP_005770187.1 DNA repair protein RadA Machinery gene
  GRB21_RS24835 mscL 5244766..5245212 (+) 447 WP_007243783.1 large-conductance mechanosensitive channel protein MscL -
  GRB21_RS24840 - 5245412..5246188 (-) 777 WP_011105097.1 ferredoxin--NADP reductase -
  GRB21_RS24845 - 5246622..5247599 (-) 978 WP_007247571.1 IS5-like element ISPssy family transposase -
  GRB21_RS24850 - 5247718..5247951 (+) 234 WP_005770190.1 helix-turn-helix domain-containing protein -
  GRB21_RS24855 - 5248081..5249205 (+) 1125 WP_005770191.1 methyltransferase -
  GRB21_RS24860 - 5249293..5249460 (-) 168 WP_003381744.1 DUF2474 domain-containing protein -

Sequence

Protein


Download         Length: 455 a.a.        Molecular weight: 48838.42 Da        Isoelectric Point: 7.4045

>NTDB_id=410168 GRB21_RS24830 WP_005770187.1 5243204..5244571(-) (radA) [Pseudomonas syringae pv. tomato strain delta X]
MAKAKRLYGCTECGATFPKWAGQCADCGAWNTLVETMIESGAAAPPSGRTGWTGSQAQIRTLAQVSVEEIPRFSTNSAEL
DRVLGGGLVDGSVVLIGGDPGIGKSTILLQTLCNIAQRMPALYVTGEESQQQVAMRARRLGLPQDKLRVMTETCIETIIA
TAKVEKPKVMVIDSIQTIFTEQLQSAPGGVSQVRESAALLVRYAKQSGTAIFLVGHVTKEGALAGPRVLEHMVDTVLYFE
GESDGRLRLLRAVKNRFGAVNELGVFGMTDKGLKEVSNPSAIFLTRAQEEVPGSVVMATWEGTRPMLVEVQALVDDSHMS
NPRRVTLGLDQNRLAMLLAVLHRHGGIPTHDQDVFLNVVGGVKVLETASDLALMAAVMSSLRNRPLPHDLLVFGEVGLSG
EVRPVPSGQERLKEAAKHGFKRAIVPKGNAPKEAPPGLQIIAVTRLEQALDALFE

Nucleotide


Download         Length: 1368 bp        

>NTDB_id=410168 GRB21_RS24830 WP_005770187.1 5243204..5244571(-) (radA) [Pseudomonas syringae pv. tomato strain delta X]
ATGGCCAAGGCCAAACGCTTGTACGGCTGCACCGAATGCGGCGCGACATTTCCCAAGTGGGCGGGCCAGTGTGCTGATTG
CGGCGCCTGGAACACACTGGTCGAAACCATGATCGAAAGCGGTGCCGCCGCGCCTCCCAGCGGGCGCACCGGCTGGACCG
GCTCACAGGCGCAGATCAGAACCCTGGCGCAAGTCAGCGTTGAAGAGATCCCGCGTTTCTCCACCAACTCCGCCGAACTC
GACAGGGTGCTCGGTGGCGGTCTGGTGGATGGTTCTGTGGTGCTGATCGGCGGCGACCCTGGCATCGGCAAGTCGACGAT
TCTGTTGCAAACGCTGTGCAACATCGCCCAGCGCATGCCTGCGCTGTATGTCACCGGCGAGGAATCCCAACAGCAGGTGG
CCATGCGCGCCCGCCGTCTGGGCCTGCCGCAGGACAAACTGCGGGTCATGACCGAAACCTGCATCGAAACCATTATTGCC
ACCGCCAAGGTCGAGAAGCCCAAGGTGATGGTGATTGACTCGATCCAGACGATCTTCACCGAGCAATTGCAGTCCGCACC
GGGTGGCGTGTCGCAAGTGCGCGAAAGCGCCGCCTTGCTGGTGCGCTATGCCAAGCAGAGTGGCACCGCGATTTTCCTGG
TGGGGCATGTCACCAAGGAAGGCGCACTGGCGGGGCCGCGTGTGCTTGAGCACATGGTCGATACCGTGCTGTATTTCGAG
GGCGAGTCGGACGGTCGTCTGCGTTTGCTGCGCGCTGTAAAGAACCGTTTCGGCGCGGTAAACGAACTGGGTGTGTTCGG
CATGACCGACAAGGGCCTGAAAGAAGTGTCCAATCCGTCGGCGATTTTTCTGACCCGTGCGCAGGAAGAAGTCCCCGGCA
GCGTGGTCATGGCCACATGGGAAGGCACCCGGCCGATGCTGGTCGAAGTTCAGGCGCTGGTGGATGACAGCCATATGTCC
AATCCGCGCCGCGTAACCCTGGGCCTGGATCAGAACCGTCTGGCGATGCTGCTGGCGGTGCTGCACCGGCATGGCGGCAT
TCCAACCCACGACCAGGACGTGTTCCTCAATGTGGTGGGCGGGGTCAAAGTGCTGGAAACCGCGTCCGATCTGGCCTTGA
TGGCCGCAGTCATGTCCAGCCTGCGCAATCGCCCGCTGCCGCATGACTTGCTGGTGTTTGGCGAGGTGGGGCTGTCGGGT
GAGGTCAGGCCGGTGCCCAGCGGGCAGGAGCGCTTGAAAGAGGCTGCCAAGCACGGCTTCAAACGGGCCATCGTGCCGAA
AGGCAATGCGCCGAAAGAAGCGCCTCCCGGTCTGCAGATCATTGCGGTCACGCGTCTGGAACAGGCGCTGGATGCGCTGT
TCGAGTAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(353-429)

AAA domain (AAA_25)

(73-221)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q87WB3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

49.123

100

0.492
  radA Streptococcus pneumoniae Rx1

46.522

100

0.47
  radA Streptococcus pneumoniae R6

46.522

100

0.47
  radA Streptococcus pneumoniae TIGR4

46.522

100

0.47
  radA Streptococcus pneumoniae D39

46.522

100

0.47
  radA Streptococcus mitis SK321

46.711

100

0.468
  radA Streptococcus mitis NCTC 12261

46.491

100

0.466

Multiple sequence alignment