Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   DESRU_RS01375 Genome accession   NC_015589
Coordinates   268843..270195 (+) Length   450 a.a.
NCBI ID   WP_013840338.1    Uniprot ID   F6DNM1
Organism   Desulforamulus ruminis DSM 2154     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 263843..275195
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DESRU_RS01355 (Desru_0260) - 264091..264555 (+) 465 WP_013840334.1 CtsR family transcriptional regulator -
  DESRU_RS01360 (Desru_0261) - 264577..265089 (+) 513 WP_013840335.1 UvrB/UvrC motif-containing protein -
  DESRU_RS01365 (Desru_0262) - 265102..266172 (+) 1071 WP_013840336.1 protein arginine kinase -
  DESRU_RS01370 (Desru_0263) - 266184..268631 (+) 2448 WP_013840337.1 ATP-dependent Clp protease ATP-binding subunit -
  DESRU_RS01375 (Desru_0264) radA 268843..270195 (+) 1353 WP_013840338.1 DNA repair protein RadA Machinery gene
  DESRU_RS01380 (Desru_0265) disA 270207..271292 (+) 1086 WP_013840339.1 DNA integrity scanning diadenylate cyclase DisA -
  DESRU_RS01385 (Desru_0266) - 271363..271758 (-) 396 WP_013840340.1 hypothetical protein -
  DESRU_RS01390 (Desru_0267) - 271950..272426 (+) 477 WP_013840341.1 CarD family transcriptional regulator -
  DESRU_RS01395 (Desru_0268) - 272544..273683 (+) 1140 WP_013840342.1 PIN/TRAM domain-containing protein -
  DESRU_RS01400 (Desru_0269) ispD 273676..274371 (+) 696 WP_013840343.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  DESRU_RS01405 (Desru_0270) ispF 274442..274918 (+) 477 WP_013840344.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 450 a.a.        Molecular weight: 48243.78 Da        Isoelectric Point: 7.4655

>NTDB_id=41009 DESRU_RS01375 WP_013840338.1 268843..270195(+) (radA) [Desulforamulus ruminis DSM 2154]
MRNKTTYACQQCGHQTARWMGRCPGCGEWNSLVEEKMVSPAKTVRLSPLSAPIPVTDVAATEEERYATGLKELDRALGGG
LVPGSLVLVGGDPGIGKSTLLLQAAAALAEKVGTVLYVSGEESARQIKMRAQRINALHPNILLMAETDISVVERHIHELS
PAAVVLDSIQTAFQPEISSTPGSVAQVRECTGVLMRVAKGTSIPVFLVGHVTKEGTLAGPRVLEHMVDTVLYFEGDRHQS
FRILRTVKNRFGSAHELGIFEMRDFGLIEVSNPSALFMGQSSRGVAGSVIVPSLEGTRPLLVEIQALVCASGFGTPRRMT
AGVDPNRVALIMAVLERRVGLRLAAHDAYVSAMGGVKLDEPAVDLAIALAIASSFRERPVQDRLVVLGEVGLTGEIRAVS
GLEKRLQEAAKLGFSSCLGPLWKHQRDSLGIINYIEVQSIHEAVEAALGR

Nucleotide


Download         Length: 1353 bp        

>NTDB_id=41009 DESRU_RS01375 WP_013840338.1 268843..270195(+) (radA) [Desulforamulus ruminis DSM 2154]
TTGCGCAACAAAACAACCTATGCTTGCCAGCAGTGCGGACATCAGACCGCCCGGTGGATGGGGCGCTGTCCGGGCTGCGG
CGAATGGAACTCCCTGGTGGAGGAGAAAATGGTCTCCCCGGCTAAAACAGTACGCTTATCCCCCCTTTCGGCTCCGATTC
CCGTGACCGATGTGGCAGCCACGGAGGAGGAACGATACGCCACCGGGTTGAAGGAACTGGACCGGGCTCTTGGCGGGGGT
CTGGTACCCGGTTCCCTGGTGCTGGTTGGCGGGGATCCGGGTATCGGCAAATCCACCCTGCTGCTGCAGGCCGCTGCTGC
CCTGGCTGAAAAGGTTGGCACCGTGCTTTACGTTTCCGGCGAAGAATCCGCCCGGCAGATTAAAATGAGGGCGCAACGGA
TTAATGCTTTGCATCCCAATATATTACTGATGGCAGAGACGGATATTTCGGTAGTGGAACGGCATATTCATGAATTATCC
CCTGCGGCGGTGGTTCTGGACTCCATTCAAACCGCCTTTCAGCCGGAGATTTCCTCAACGCCCGGAAGTGTCGCCCAGGT
TAGGGAATGTACCGGGGTTTTGATGAGAGTGGCCAAGGGTACAAGTATCCCGGTTTTTCTGGTTGGGCATGTTACTAAAG
AAGGGACCCTGGCCGGACCCAGGGTGCTGGAGCATATGGTGGATACGGTGCTTTATTTTGAAGGGGACCGGCACCAGTCC
TTCCGGATTCTGCGCACCGTAAAGAACCGTTTTGGCTCGGCCCATGAGCTGGGTATCTTTGAAATGCGGGATTTTGGACT
GATAGAAGTGAGCAATCCCTCGGCGTTATTTATGGGTCAGAGTTCCCGGGGAGTGGCGGGATCGGTGATTGTTCCCAGCC
TGGAAGGAACCAGGCCTCTTTTGGTGGAGATACAGGCGCTGGTATGTGCCAGTGGTTTTGGGACGCCAAGAAGGATGACG
GCGGGAGTGGACCCCAACCGGGTGGCTTTAATTATGGCTGTTCTGGAACGCAGGGTAGGACTCCGGCTGGCGGCCCATGA
TGCCTATGTTAGTGCCATGGGGGGCGTCAAACTGGACGAACCCGCAGTGGATTTAGCCATTGCCCTGGCCATCGCCTCCA
GTTTCAGGGAAAGACCGGTGCAGGACCGGCTGGTGGTTTTGGGAGAAGTAGGTCTAACCGGTGAAATTAGGGCTGTAAGC
GGTTTGGAAAAGCGACTTCAGGAAGCTGCCAAATTGGGATTTAGCAGTTGTCTGGGACCCCTTTGGAAACATCAAAGGGA
CTCCCTGGGTATAATAAATTATATAGAGGTTCAATCCATTCACGAAGCTGTGGAGGCTGCCCTAGGGCGGTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(62-215)

Subunit ChlI of Mg-chelatase (ChlI)

(360-418)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(7-34)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB F6DNM1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

54.783

100

0.56
  radA Streptococcus mitis SK321

54.176

98.444

0.533
  radA Streptococcus mitis NCTC 12261

54.176

98.444

0.533
  radA Streptococcus pneumoniae Rx1

55.213

93.778

0.518
  radA Streptococcus pneumoniae D39

55.213

93.778

0.518
  radA Streptococcus pneumoniae R6

55.213

93.778

0.518
  radA Streptococcus pneumoniae TIGR4

55.213

93.778

0.518

Multiple sequence alignment