Detailed information NTG0040985

Bioinformatically predicted

Overview

• Name: pilA
• Type: Machinery gene
• Locus tag: THICY_RS01395
• Genome accession: NC_015581
• Coordinates: 270957..272003 (-)
• Length: 348 a.a.
• NCBI ID: WP_013834827.1
• Uniprot ID: F6DA07
• Organism: Thiomicrospira cyclica ALM1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 265957..277003

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
THICY_RS08570 (Thicy_0263)	-	266463..267284 (+)	822	WP_013834822.1	biotin--[acetyl-CoA-carboxylase] ligase	-
THICY_RS01370 (Thicy_0264)	-	267265..268098 (+)	834	WP_157862705.1	type III pantothenate kinase	-
THICY_RS01380 (Thicy_0265)	fabA	268374..268889 (+)	516	WP_013834824.1	3-hydroxyacyl-[acyl-carrier-protein] dehydratase FabA	-
THICY_RS01385 (Thicy_0266)	fabB	268891..270111 (+)	1221	WP_013834825.1	beta-ketoacyl-ACP synthase I	-
THICY_RS01390 (Thicy_0267)	-	270221..270847 (-)	627	WP_013834826.1	membrane protein	-
THICY_RS01395 (Thicy_0268)	pilA	270957..272003 (-)	1047	WP_013834827.1	signal recognition particle-docking protein FtsY	Machinery gene
THICY_RS01400 (Thicy_0269)	-	272119..273498 (+)	1380	WP_013834828.1	pitrilysin family protein	-
THICY_RS01405 (Thicy_0270)	-	273498..274850 (+)	1353	WP_013834829.1	pitrilysin family protein	-
THICY_RS01410 (Thicy_0271)	rsmD	274837..275484 (+)	648	WP_013834830.1	16S rRNA (guanine(966)-N(2))-methyltransferase RsmD	-
THICY_RS01415 (Thicy_0272)	coaD	275530..276012 (+)	483	WP_013834831.1	pantetheine-phosphate adenylyltransferase	-
THICY_RS01420 (Thicy_0273)	-	276002..276781 (-)	780	WP_013834832.1	DUF4395 domain-containing protein	-

Sequence

Protein

• Length: 348 a.a.
• Molecular weight: 37632.13 Da
• Isoelectric Point: 5.1148

>NTDB_id=40985 THICY_RS01395 WP_013834827.1 270957..272003(-) (pilA) [Thiomicrospira cyclica ALM1]
MFDFFKKKPIVTETPPEEVSNVAEVSEVSQETGETPTKMGLFARLKSQLTKSREAFTSGLSQLFLGKKQIDDELLEELET
LLLQADVGFEATTQIIDALTEQVSRQALKDPQALFEALASQLEAILTPVEQALVINSDAKPHVVLMVGINGAGKTTTIGK
LAKFLQRQQHKVMLAAGDTFRAAAVEQLQTWGERNQIAVMAQKTGADSAAVIFDAIHSAQAKNLDVLLADTAGRLHTQHN
LMDELKKVKRVIQKCDADAPHDVLLVLDASIGQNAINQAKQFHEAVGVTGLVITKLDGTAKGGVIFNLAQTLGLPVKFIG
VGEKIDDLQVFNAKAFVDAFLDSPASEH

Nucleotide

• Length: 1047 bp

>NTDB_id=40985 THICY_RS01395 WP_013834827.1 270957..272003(-) (pilA) [Thiomicrospira cyclica ALM1]
ATGTTCGATTTTTTTAAAAAGAAACCTATTGTAACTGAGACACCCCCTGAAGAGGTTAGCAATGTCGCCGAGGTGAGCGA
AGTCTCGCAAGAGACTGGGGAGACGCCAACGAAAATGGGGCTGTTTGCGCGACTAAAATCCCAACTGACAAAATCACGCG
AAGCCTTTACGTCGGGATTATCGCAGTTATTTTTAGGTAAAAAACAGATTGATGACGAACTGCTTGAGGAGCTGGAAACC
CTGTTATTGCAGGCGGATGTTGGCTTTGAGGCAACGACCCAAATTATTGATGCGCTGACTGAGCAGGTGTCGCGTCAGGC
GTTAAAAGATCCACAAGCCTTGTTTGAAGCCTTGGCCAGTCAACTCGAGGCGATTTTGACGCCCGTTGAACAGGCGTTGG
TGATTAACAGTGATGCCAAACCTCACGTGGTGTTGATGGTGGGCATTAATGGCGCCGGTAAAACCACCACCATTGGTAAG
CTGGCTAAGTTTTTACAACGCCAACAACATAAGGTGATGTTGGCAGCGGGCGATACATTCCGGGCCGCGGCGGTGGAACA
GTTGCAAACTTGGGGCGAGCGGAATCAAATTGCAGTAATGGCACAAAAAACCGGTGCCGATTCTGCGGCGGTTATTTTTG
ATGCCATCCATTCAGCACAGGCCAAAAACTTAGATGTGTTGCTAGCTGACACGGCCGGGCGTTTGCATACGCAACATAAT
TTGATGGACGAACTTAAGAAGGTTAAGCGCGTTATTCAGAAGTGTGATGCCGATGCGCCGCATGATGTATTGCTGGTGTT
GGATGCCTCGATTGGTCAAAATGCGATTAATCAGGCTAAGCAGTTTCATGAAGCAGTTGGCGTAACAGGCCTGGTGATTA
CGAAGTTGGACGGTACGGCTAAGGGCGGCGTTATTTTTAATTTGGCGCAAACGCTGGGCTTGCCGGTGAAGTTTATTGGT
GTTGGTGAGAAAATTGATGATCTGCAAGTCTTTAATGCGAAGGCGTTTGTGGACGCATTTTTAGATAGCCCGGCTTCTGA
ACATTAA

Domains

Predicted by InterproScan.

• SRP54-type protein, GTPase domain (SRP54) (141-341)

• SRP54-type protein, helical bundle domain (SRP54_N) (56-121)

3D structure

• AlphaFold DB: F6DA07

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA	Neisseria gonorrhoeae MS11	52.836	96.264	0.509