Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   H0271_RS20885 Genome accession   NZ_CP059279
Coordinates   4332690..4333499 (-) Length   269 a.a.
NCBI ID   WP_001547020.1    Uniprot ID   -
Organism   Escherichia coli strain C7     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4327690..4338499
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H0271_RS20870 (H0271_20870) gspD 4329147..4331207 (-) 2061 WP_000498829.1 type II secretion system secretin GspD -
  H0271_RS20875 (H0271_20875) gspC 4331237..4332196 (-) 960 WP_103818134.1 type II secretion system protein GspC -
  H0271_RS20880 (H0271_20880) gspS2 4332214..4332624 (-) 411 WP_001300497.1 type II secretion system pilot lipoprotein GspS-beta -
  H0271_RS20885 (H0271_20885) pilD 4332690..4333499 (-) 810 WP_001547020.1 prepilin peptidase PppA Machinery gene
  H0271_RS20890 (H0271_20890) sslE 4333697..4338253 (-) 4557 WP_103818133.1 lipoprotein metalloprotease SslE -

Sequence

Protein


Download         Length: 269 a.a.        Molecular weight: 29446.02 Da        Isoelectric Point: 8.3829

>NTDB_id=409334 H0271_RS20885 WP_001547020.1 4332690..4333499(-) (pilD) [Escherichia coli strain C7]
MLFDVFQQYPAAMPVLATVGGLIIGSFLNVVIWRYPIMLRQQMAEFHGEMPSAQSKISLALPRSHCPHCQQTIRVRDNIP
LFSWLMLKGRCRDCQAKISKRYPLVELLTALAFLLASLVWPESGWALAVMILSAWLIAASVIDLDHQWLPDVFTQGVLWT
GLSAAWAQQSPLTLQDAVTGVLVGFIAFYSLRWIAGIVLRKEALGMGDVLLFAALGSWVGPLSLPNVALIASCCGLIYAV
ITKRGSTTLPFGPCLSLGGIATIYLQALF

Nucleotide


Download         Length: 810 bp        

>NTDB_id=409334 H0271_RS20885 WP_001547020.1 4332690..4333499(-) (pilD) [Escherichia coli strain C7]
ATGCTTTTTGATGTTTTTCAGCAATACCCTGCGGCGATGCCCGTCCTGGCAACCGTCGGAGGATTGATTATAGGTAGTTT
TTTGAATGTGGTGATTTGGCGTTACCCCATCATGCTGCGCCAACAAATGGCGGAGTTTCACGGTGAAATGCCGAGTGCGC
AGTCAAAAATAAGCCTGGCGCTGCCACGTTCGCACTGTCCACATTGTCAGCAGACCATCCGCGTTCGTGACAATATTCCG
CTGTTCTCCTGGCTGATGCTCAAAGGGCGCTGCCGCGACTGTCAGGCGAAAATCAGCAAGCGTTATCCGCTGGTGGAGTT
ATTGACGGCACTCGCTTTTTTGCTGGCGAGTCTGGTCTGGCCGGAAAGTGGATGGGCGCTGGCGGTGATGATATTATCCG
CCTGGCTGATTGCCGCGAGCGTCATTGACCTCGATCACCAATGGCTGCCCGATGTTTTTACTCAGGGCGTATTGTGGACG
GGACTGAGTGCGGCATGGGCGCAGCAGAGCCCGCTCACGCTACAAGATGCAGTTACCGGCGTCCTGGTGGGGTTTATCGC
TTTTTACTCCCTGCGCTGGATAGCCGGAATAGTTCTGCGTAAAGAAGCATTAGGCATGGGCGATGTATTATTGTTCGCTG
CGTTAGGTAGTTGGGTGGGGCCGTTGTCGCTACCCAATGTTGCTTTAATCGCATCATGCTGCGGCCTGATATATGCCGTT
ATTACAAAAAGAGGATCAACCACACTGCCTTTTGGACCGTGTTTAAGTCTGGGCGGTATAGCAACAATTTATCTACAGGC
ATTGTTTTAA

Domains

Predicted by InterProScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(21-118)

Type IV leader peptidase family (Peptidase_A24)

(130-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

46.067

99.257

0.457
  pilD Vibrio campbellii strain DS40M4

42.322

99.257

0.42
  pilD Acinetobacter nosocomialis M2

38.113

98.513

0.375
  pilD Neisseria gonorrhoeae MS11

38.911

95.539

0.372
  pilD Acinetobacter baumannii D1279779

37.736

98.513

0.372

Multiple sequence alignment