Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   FAZ97_RS01540 Genome accession   NZ_CP046909
Coordinates   326918..328435 (-) Length   505 a.a.
NCBI ID   WP_158756866.1    Uniprot ID   A0A7Z2J6Q6
Organism   Paraburkholderia acidiphila strain 7Q-K02     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 321918..333435
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FAZ97_RS01515 (FAZ97_01515) - 322732..323367 (-) 636 WP_158756864.1 LON peptidase substrate-binding domain-containing protein -
  FAZ97_RS01520 (FAZ97_01520) rapZ 323410..324303 (-) 894 WP_158756865.1 RNase adapter RapZ -
  FAZ97_RS01525 (FAZ97_01525) hprK 324442..325410 (-) 969 WP_028201859.1 HPr(Ser) kinase/phosphatase -
  FAZ97_RS01530 (FAZ97_01530) ptsN 325579..326034 (-) 456 WP_028201860.1 PTS IIA-like nitrogen regulatory protein PtsN -
  FAZ97_RS01535 (FAZ97_01535) hpf 326388..326744 (-) 357 WP_028201861.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  FAZ97_RS01540 (FAZ97_01540) rpoN/rpoN1 326918..328435 (-) 1518 WP_158756866.1 RNA polymerase factor sigma-54 Machinery gene
  FAZ97_RS01545 (FAZ97_01545) lptB 328715..329497 (-) 783 WP_158759013.1 LPS export ABC transporter ATP-binding protein -
  FAZ97_RS01550 (FAZ97_01550) lptA 329503..330195 (-) 693 WP_158756867.1 lipopolysaccharide transport periplasmic protein LptA -
  FAZ97_RS01555 (FAZ97_01555) lptC 330227..330826 (-) 600 WP_158756868.1 LPS export ABC transporter periplasmic protein LptC -
  FAZ97_RS01560 (FAZ97_01560) - 330823..331377 (-) 555 WP_158756869.1 HAD family hydrolase -
  FAZ97_RS01565 (FAZ97_01565) kdsD 331377..332363 (-) 987 WP_158756870.1 arabinose 5-phosphate isomerase KdsD -

Sequence

Protein


Download         Length: 505 a.a.        Molecular weight: 55379.32 Da        Isoelectric Point: 4.9485

>NTDB_id=409292 FAZ97_RS01540 WP_158756866.1 326918..328435(-) (rpoN/rpoN1) [Paraburkholderia acidiphila strain 7Q-K02]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVATAIAQNPLLENEDDWIASPLRVAADGSVIAQPPTPSGNEPMQ
GAAGSTTASNGSGENSESEPRGVDEYDGLGGDANGDASQWNLDDYGRSGNASDDDDLPPLQIHESTTTLRDHLMAQLRET
NASQRDRALITFLIESLDEDGYLGATCEEILADIPEELEVDVDEFNAALALLHSFDPAGVGARSASECLKLQLLRVNSSP
TRTLALEIVSNHLELLAARDFTRLRKQLKTGDDELRDAHAMIRSLEPFPGASYGKAEADYVVPDIIVKKAGQNWHAELNP
EVVPKLRINHLYANILRNNRGDPGSGSLRQQLQEARWLIKNIQQRFETILRVAQAIVERQKNFFVHGEIAMRPLVLREIA
DTLGLHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAENTKSPLSDSRIAELLAEQGF
VVARRTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1518 bp        

>NTDB_id=409292 FAZ97_RS01540 WP_158756866.1 326918..328435(-) (rpoN/rpoN1) [Paraburkholderia acidiphila strain 7Q-K02]
ATGAAAGCCAGCCTCCAACTCCGCCTCTCGCAGCATCTTGCGCTCACGCCACAACTGCAACAGTCCATCCGGCTGCTGCA
GCTGTCGACGCTCGAACTGCAGCAGGAGGTCGCGACGGCGATCGCGCAGAACCCGCTGCTCGAGAACGAGGACGACTGGA
TCGCAAGCCCGCTGCGCGTGGCCGCCGACGGCTCGGTCATCGCGCAGCCGCCCACGCCTTCGGGCAACGAGCCGATGCAG
GGCGCGGCCGGCTCGACCACGGCATCCAACGGCAGCGGCGAAAACAGCGAAAGCGAGCCGCGCGGGGTGGACGAGTACGA
CGGCCTCGGCGGCGACGCAAACGGCGACGCGAGCCAGTGGAACCTGGACGACTACGGCCGCTCCGGCAACGCATCCGACG
ACGACGATCTGCCGCCGCTGCAAATCCACGAATCGACCACCACACTGCGCGACCATCTGATGGCGCAGCTGCGCGAAACG
AACGCGAGCCAGCGCGACCGTGCGCTGATCACATTCCTCATCGAATCGCTCGACGAAGACGGTTACCTCGGCGCGACCTG
TGAGGAAATTCTTGCCGACATCCCCGAAGAACTCGAAGTCGACGTCGACGAATTCAATGCAGCGTTAGCACTGCTGCATA
GCTTCGACCCCGCGGGCGTGGGTGCGCGTTCGGCCTCCGAGTGCCTCAAGCTGCAACTTTTGCGCGTCAATTCGTCGCCC
ACGCGCACGCTCGCGCTCGAGATCGTGTCGAACCATCTGGAACTGCTCGCCGCGCGCGATTTCACGCGGTTGCGCAAGCA
ACTGAAGACGGGCGACGACGAGCTGCGCGATGCGCATGCGATGATCCGCTCGCTCGAACCGTTCCCGGGCGCGTCGTATG
GCAAGGCCGAAGCCGACTACGTGGTGCCTGACATCATCGTGAAGAAGGCCGGCCAGAACTGGCATGCCGAACTGAACCCG
GAGGTGGTGCCGAAGCTGCGCATCAACCATCTGTACGCGAATATTCTGCGCAATAACCGCGGCGACCCGGGCAGCGGTTC
GCTGCGCCAACAGCTGCAGGAAGCGCGCTGGCTCATCAAGAATATCCAGCAGCGTTTCGAGACGATCCTGCGTGTCGCGC
AGGCTATCGTCGAGCGTCAGAAGAACTTTTTCGTGCACGGCGAAATCGCCATGCGCCCCTTGGTTTTGAGGGAGATTGCT
GATACGCTGGGCCTACACGAATCAACTGTCTCGCGTGTGACAACCGGTAAATACATGCTGACTCCATTCGGGACACTTGA
ATTTAAGTACTTCTTCGGATCGCATGTTTCAACGGACACCGGAGGCGCTGCATCGTCCACCGCCATCCGCGCCCTCATCA
AGCAACTCATAGGAGCCGAGAACACCAAATCCCCTCTTTCAGACAGCCGCATCGCCGAACTGCTGGCGGAACAGGGCTTC
GTGGTCGCGCGACGCACAGTCGCGAAGTATCGTGAGGCGCTGAAGATTCCGGCGGTCAATCTGCGCAAGTCTCTTTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(142-332)

Sigma-54, DNA binding domain (Sigma54_DBD)

(348-503)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7Z2J6Q6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

64.453

100

0.653

Multiple sequence alignment