Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   METME_RS16640 Genome accession   NC_015572
Coordinates   3657339..3657791 (-) Length   150 a.a.
NCBI ID   WP_013819917.1    Uniprot ID   G0A5I4
Organism   Methylomonas methanica MC09     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3652339..3662791
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  METME_RS16620 (Metme_3315) - 3653422..3653844 (-) 423 WP_081470831.1 phosphate-starvation-inducible PsiE family protein -
  METME_RS16625 (Metme_3316) - 3653849..3654781 (-) 933 WP_013819914.1 sulfite exporter TauE/SafE family protein -
  METME_RS16630 (Metme_3317) - 3654791..3655489 (-) 699 WP_013819915.1 MBL fold metallo-hydrolase -
  METME_RS16635 (Metme_3318) - 3655760..3657184 (-) 1425 WP_013819916.1 O-antigen ligase family protein -
  METME_RS16640 (Metme_3319) pilA 3657339..3657791 (-) 453 WP_013819917.1 pilin Machinery gene
  METME_RS16645 (Metme_3320) - 3658121..3658474 (+) 354 WP_013819918.1 ArsC family reductase -
  METME_RS16650 (Metme_3321) dapE 3658471..3659598 (+) 1128 WP_013819919.1 succinyl-diaminopimelate desuccinylase -
  METME_RS16655 (Metme_3322) radC 3659649..3660323 (-) 675 WP_013819920.1 DNA repair protein RadC -
  METME_RS16660 (Metme_3323) coaBC 3660415..3661623 (+) 1209 WP_041364498.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -
  METME_RS16665 (Metme_3324) dut 3661595..3662050 (+) 456 WP_013819922.1 dUTP diphosphatase -

Sequence

Protein


Download         Length: 150 a.a.        Molecular weight: 15351.73 Da        Isoelectric Point: 6.1906

>NTDB_id=40929 METME_RS16640 WP_013819917.1 3657339..3657791(-) (pilA) [Methylomonas methanica MC09]
MNMQMKKVQQGFTLIELMIVVAIIGILAAVAIPAYQDYTIRAKVTEGLSLASAAKLAVADAYAAGVATASLDSTADGSGP
LGYKQPAATTQVSSITVADGVISVSFNDLGGGVQPNLTLAPTFSAGEPIQWLCRPAVAASSKYMPANCRS

Nucleotide


Download         Length: 453 bp        

>NTDB_id=40929 METME_RS16640 WP_013819917.1 3657339..3657791(-) (pilA) [Methylomonas methanica MC09]
ATGAACATGCAAATGAAAAAAGTACAACAAGGTTTTACCTTGATCGAATTAATGATCGTTGTGGCGATCATCGGTATTTT
GGCGGCGGTGGCAATACCGGCCTACCAGGATTATACAATTAGGGCAAAAGTTACCGAGGGCTTAAGTTTAGCTAGTGCCG
CTAAATTAGCTGTAGCGGATGCTTATGCAGCTGGTGTTGCAACTGCTAGTTTAGACTCAACTGCCGATGGCAGTGGTCCT
CTAGGGTATAAACAGCCGGCGGCAACAACACAAGTTTCAAGTATTACAGTAGCTGATGGCGTCATTTCTGTATCATTTAA
CGACTTAGGTGGCGGCGTGCAACCAAATTTGACATTAGCACCAACATTTTCCGCCGGTGAGCCTATTCAATGGTTGTGCA
GACCAGCTGTTGCAGCAAGCAGTAAATATATGCCTGCAAACTGCAGATCATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)

Pilin (bacterial filament) (Pilin)

(41-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G0A5I4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

43.82

100

0.52
  pilA2 Legionella pneumophila strain ERS1305867

50.704

94.667

0.48
  pilA2 Legionella pneumophila str. Paris

50.704

94.667

0.48
  comP Acinetobacter baylyi ADP1

45.946

98.667

0.453
  pilE Neisseria gonorrhoeae strain FA1090

36

100

0.42
  pilE Neisseria gonorrhoeae MS11

36.527

100

0.407
  pilA/pilA1 Eikenella corrodens VA1

37.888

100

0.407
  pilA/pilAI Pseudomonas stutzeri DSM 10701

39.865

98.667

0.393
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

35.404

100

0.38
  pilA Vibrio cholerae strain A1552

35.404

100

0.38
  pilA Vibrio cholerae C6706

35.404

100

0.38
  pilA Pseudomonas aeruginosa PAK

35.714

100

0.367

Multiple sequence alignment