Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   GO485_RS20930 Genome accession   NZ_CP046904
Coordinates   4860822..4861880 (-) Length   352 a.a.
NCBI ID   WP_145872663.1    Uniprot ID   A0A562Q3C3
Organism   Pseudoduganella flava strain DSM 26639     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4855822..4866880
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GO485_RS20910 (GO485_20865) - 4857236..4857694 (-) 459 WP_145872660.1 hypothetical protein -
  GO485_RS20915 (GO485_20870) rpoH 4857886..4858779 (-) 894 WP_145873256.1 RNA polymerase sigma factor RpoH -
  GO485_RS20920 (GO485_20875) ftsX 4859233..4860147 (-) 915 WP_145872661.1 permease-like cell division protein FtsX -
  GO485_RS20925 (GO485_20880) - 4860144..4860815 (-) 672 WP_145872662.1 cell division ATP-binding protein FtsE -
  GO485_RS20930 (GO485_20885) pilA 4860822..4861880 (-) 1059 WP_145872663.1 signal recognition particle-docking protein FtsY Machinery gene
  GO485_RS20935 (GO485_20890) - 4862020..4862973 (-) 954 WP_145872664.1 MerR family transcriptional regulator -
  GO485_RS20940 (GO485_20895) - 4863191..4864108 (-) 918 WP_145872665.1 AEC family transporter -
  GO485_RS20945 (GO485_20900) - 4864126..4865100 (-) 975 WP_145872666.1 TauD/TfdA family dioxygenase -

Sequence

Protein


Download         Length: 352 a.a.        Molecular weight: 37618.58 Da        Isoelectric Point: 6.5114

>NTDB_id=409261 GO485_RS20930 WP_145872663.1 4860822..4861880(-) (pilA) [Pseudoduganella flava strain DSM 26639]
MFSFFKKKTVTPEVPPAPVQQPAPAAAEPAAAPDLPPDTAPLAFEMETAPRPESKQSWMSRLKAGLSKTSANLSLLFVGA
RIDDELYEELEAALLMSDAGIDATHYLLDALKRKVKDEKLTDAAAVKAALKTLMIDLLRPLEKPFVLGRHQPTVMMISGV
NGAGKTTTIGKLAKHMQAHGQSVLLAAGDTFRAAAREQLMVWGQRNNVTVISQESGDPAAVSYDAVQSGKAKGTNVVMID
TAGRLPTQLHLMEELKKIKRVIGKGMEGAPHETLLVIDGNTGQNALAQVKAFDDALQLSGLIITKLDGTAKGGVIAAIAR
VRPVPVYFIGIGEKIEDLQPFDAEEFVEALLG

Nucleotide


Download         Length: 1059 bp        

>NTDB_id=409261 GO485_RS20930 WP_145872663.1 4860822..4861880(-) (pilA) [Pseudoduganella flava strain DSM 26639]
ATGTTCAGCTTCTTCAAGAAAAAAACCGTCACGCCGGAGGTGCCGCCCGCACCGGTGCAACAGCCGGCGCCCGCCGCCGC
AGAGCCTGCGGCCGCCCCCGACCTTCCCCCGGACACCGCCCCGCTCGCGTTCGAGATGGAAACGGCGCCGCGGCCGGAAT
CGAAACAATCCTGGATGAGCCGGCTGAAGGCCGGCCTGTCGAAGACCTCCGCCAACCTGTCGCTGCTGTTTGTCGGTGCC
CGCATCGACGACGAGCTGTACGAAGAGCTGGAGGCGGCGCTGCTGATGTCCGATGCCGGCATCGACGCGACCCACTACCT
GCTCGATGCGTTGAAACGCAAGGTCAAGGACGAGAAGCTGACCGACGCCGCCGCCGTCAAGGCCGCGCTGAAGACGTTGA
TGATCGACCTGCTCCGGCCGCTGGAAAAACCGTTCGTGCTGGGCCGTCACCAGCCGACCGTGATGATGATTTCCGGCGTC
AACGGCGCCGGCAAGACGACGACGATCGGCAAGCTCGCCAAGCACATGCAGGCGCACGGCCAGTCCGTGCTGCTCGCCGC
CGGCGACACGTTCCGCGCCGCCGCGCGCGAGCAGCTGATGGTGTGGGGCCAGCGCAATAACGTCACCGTGATCTCGCAGG
AGTCCGGCGACCCGGCGGCCGTGTCGTATGACGCCGTCCAGTCCGGCAAGGCGAAAGGCACCAACGTCGTGATGATCGAT
ACGGCCGGCCGGCTGCCGACGCAGCTGCACCTGATGGAAGAGCTGAAGAAGATCAAGCGCGTGATCGGCAAGGGCATGGA
AGGCGCGCCACACGAGACGTTGCTCGTCATCGACGGCAACACGGGCCAGAACGCGCTGGCGCAGGTGAAGGCGTTCGACG
ACGCGCTGCAGCTCTCCGGCCTCATCATCACGAAGCTGGACGGCACCGCGAAAGGCGGCGTCATCGCGGCGATCGCCCGC
GTGCGCCCCGTGCCCGTGTACTTCATCGGCATCGGCGAAAAGATCGAAGACCTGCAACCCTTCGATGCCGAGGAATTCGT
CGAAGCGCTGCTCGGTTGA

Domains

Predicted by InterproScan.

SRP54-type protein, GTPase domain (SRP54)

(152-352)

SRP54-type protein, helical bundle domain (SRP54_N)

(79-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A562Q3C3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

59.74

87.5

0.523

Multiple sequence alignment