Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   METME_RS03535 Genome accession   NC_015572
Coordinates   755273..756709 (+) Length   478 a.a.
NCBI ID   WP_013817419.1    Uniprot ID   G0A4Y5
Organism   Methylomonas methanica MC09     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 750273..761709
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  METME_RS03505 (Metme_0701) - 751002..751820 (-) 819 WP_013817413.1 ATP-binding cassette domain-containing protein -
  METME_RS03510 (Metme_0702) - 751958..752938 (+) 981 WP_041364982.1 KpsF/GutQ family sugar-phosphate isomerase -
  METME_RS03515 (Metme_0703) - 752949..753482 (+) 534 WP_013817415.1 HAD hydrolase family protein -
  METME_RS03520 (Metme_0704) lptC 753479..754045 (+) 567 WP_013817416.1 LPS export ABC transporter periplasmic protein LptC -
  METME_RS03525 (Metme_0705) lptA 754032..754532 (+) 501 WP_013817417.1 lipopolysaccharide transport periplasmic protein LptA -
  METME_RS03530 (Metme_0706) lptB 754534..755259 (+) 726 WP_013817418.1 LPS export ABC transporter ATP-binding protein -
  METME_RS03535 (Metme_0707) rpoN/rpoN1 755273..756709 (+) 1437 WP_013817419.1 RNA polymerase factor sigma-54 Machinery gene
  METME_RS03540 (Metme_0708) raiA 756751..757041 (+) 291 WP_013817420.1 ribosome-associated translation inhibitor RaiA -
  METME_RS03545 (Metme_0709) rapZ 757095..757952 (+) 858 WP_013817421.1 RNase adapter RapZ -
  METME_RS03550 (Metme_0710) mgtE 757989..759344 (+) 1356 WP_013817422.1 magnesium transporter -
  METME_RS03555 (Metme_0711) minC 759421..760161 (+) 741 WP_013817423.1 septum site-determining protein MinC -
  METME_RS03560 (Metme_0712) minD 760183..760995 (+) 813 WP_013817424.1 septum site-determining protein MinD -
  METME_RS03565 (Metme_0713) minE 760995..761249 (+) 255 WP_013817425.1 cell division topological specificity factor MinE -
  METME_RS03570 (Metme_0714) - 761330..761527 (+) 198 WP_013817426.1 winged helix-turn-helix domain-containing protein -

Sequence

Protein


Download         Length: 478 a.a.        Molecular weight: 54036.20 Da        Isoelectric Point: 4.4745

>NTDB_id=40922 METME_RS03535 WP_013817419.1 755273..756709(+) (rpoN/rpoN1) [Methylomonas methanica MC09]
MKQSLQLRIGQSLTMTPQLQQAIKLLQMSTLDLQQEIQQALESNMMLEVEEDDLPTLEYREKQSENSDQVTSEGSQTDMP
DELPVDVSWDDVYESALPAGDDSSDSAEFETFRGKTQTLQDHLIWQLELTHVSERDYAIAVGIIDAINDDGYVATDLNDI
YQGLLEQLEDLEMDEVQAVLHMIQNFDPVGVGALDLADCLRLQVLQLPDDTPFKADALDFVSRHLELLATCDQARLMKRV
GFSEEQLKGVLALIRSLDPKPGARLQEMDVEYVVPDVFVHKVKDQWQVTLNPDIAPKLRINPFYSGMIKRADNSQDNVSM
KNHMQEARWFIKSLHSRNDTLLRVAKSIVEKQGEFFEHGPIAMKAMVLRDIAEELELHESTISRVTTQKYMHTPQGVIEF
KYFFSSHVSTEGGGECSATAIRALIKELVGNENPAKPLSDSKISDLLNEKGINVARRTIAKYREAMSIPSTSQRKRLI

Nucleotide


Download         Length: 1437 bp        

>NTDB_id=40922 METME_RS03535 WP_013817419.1 755273..756709(+) (rpoN/rpoN1) [Methylomonas methanica MC09]
ATGAAGCAATCTTTACAGCTCAGAATTGGTCAGAGCCTGACAATGACGCCGCAACTGCAACAGGCGATCAAATTATTGCA
GATGTCGACCTTGGATTTGCAGCAGGAAATCCAGCAGGCGTTGGAGTCCAACATGATGTTGGAAGTGGAAGAAGACGATT
TACCTACCTTGGAGTACAGGGAAAAACAGTCGGAAAATTCCGACCAAGTCACCAGCGAAGGCTCGCAAACCGATATGCCG
GATGAACTGCCGGTCGATGTCAGCTGGGACGACGTCTATGAAAGTGCCTTGCCGGCGGGAGACGATTCGTCCGACAGCGC
CGAATTCGAAACCTTTCGAGGTAAAACCCAAACCTTGCAGGATCATTTGATCTGGCAGCTGGAATTGACGCATGTTTCCG
AGCGCGACTACGCCATTGCGGTGGGGATTATCGACGCCATTAACGACGACGGTTATGTCGCCACTGATTTGAACGATATA
TACCAGGGCTTGTTGGAGCAGTTGGAAGACTTGGAAATGGATGAAGTGCAGGCCGTTTTGCACATGATTCAGAATTTCGA
TCCGGTCGGTGTCGGTGCCCTGGATTTGGCCGATTGCTTGCGTCTGCAAGTGTTGCAATTGCCGGATGATACGCCTTTCA
AGGCCGACGCCTTGGATTTCGTGTCCCGACATTTGGAGTTGTTGGCCACTTGCGATCAGGCTCGCCTGATGAAGCGGGTC
GGTTTCAGCGAGGAACAATTGAAGGGTGTCCTGGCCTTGATCAGAAGTTTGGATCCCAAGCCCGGCGCGCGCCTGCAGGA
AATGGATGTGGAGTACGTGGTGCCCGATGTATTTGTGCACAAGGTCAAGGATCAATGGCAGGTCACGCTCAATCCCGATA
TCGCACCGAAGTTGCGCATCAACCCGTTCTACTCGGGCATGATCAAGCGGGCGGACAACAGCCAGGACAATGTCAGCATG
AAAAACCACATGCAAGAGGCGCGTTGGTTTATCAAAAGCCTGCACAGCCGCAACGATACCTTGTTGCGGGTGGCCAAAAG
CATCGTGGAAAAACAGGGCGAGTTTTTCGAGCACGGTCCGATTGCCATGAAGGCCATGGTACTGAGGGATATCGCCGAGG
AACTGGAGCTGCATGAATCCACCATTTCCCGGGTTACCACACAGAAATACATGCACACCCCGCAGGGTGTAATCGAATTT
AAATATTTCTTCTCCAGTCATGTCAGCACTGAAGGGGGAGGCGAGTGCTCGGCCACGGCGATCAGAGCACTGATAAAAGA
GTTGGTGGGTAACGAAAACCCGGCCAAACCACTAAGCGATAGTAAAATATCCGACTTATTGAATGAGAAGGGCATTAATG
TGGCGCGGCGTACTATTGCAAAATACCGCGAAGCCATGTCGATTCCTTCCACCAGCCAGCGGAAAAGGCTAATTTAA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-49)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(109-304)

Sigma-54, DNA binding domain (Sigma54_DBD)

(320-476)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G0A4Y5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.635

100

0.481

Multiple sequence alignment