Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   GQO96_RS16390 Genome accession   NZ_CP046898
Coordinates   3382989..3383789 (-) Length   266 a.a.
NCBI ID   WP_187170118.1    Uniprot ID   -
Organism   Acinetobacter baumannii strain A1429     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3377989..3388789
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GQO96_RS16370 (GQO96_16285) - 3379054..3379752 (-) 699 WP_032030338.1 YfgM family protein -
  GQO96_RS16375 (GQO96_16290) hisS 3379781..3381073 (-) 1293 WP_000095253.1 histidine--tRNA ligase -
  GQO96_RS16380 (GQO96_16295) ispG 3381070..3382185 (-) 1116 WP_000572095.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  GQO96_RS16385 (GQO96_16300) - 3382207..3382998 (-) 792 WP_000405161.1 helix-turn-helix domain-containing protein -
  GQO96_RS16390 (GQO96_16305) pilF 3382989..3383789 (-) 801 WP_187170118.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  GQO96_RS16395 (GQO96_16310) rlmN 3383805..3385037 (-) 1233 WP_000093084.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  GQO96_RS16400 (GQO96_16315) ndk 3385160..3385591 (-) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  GQO96_RS16405 (GQO96_16320) iscX 3385705..3385905 (-) 201 WP_000524329.1 Fe-S cluster assembly protein IscX -
  GQO96_RS16410 (GQO96_16325) - 3386005..3386556 (-) 552 WP_000669565.1 phosphatase PAP2 family protein -
  GQO96_RS16415 (GQO96_16330) - 3386581..3387879 (-) 1299 WP_001991191.1 glycosyltransferase family 4 protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30172.55 Da        Isoelectric Point: 9.3697

>NTDB_id=409194 GQO96_RS16390 WP_187170118.1 3382989..3383789(-) (pilF) [Acinetobacter baumannii strain A1429]
MSTPKLKIMLCMGVAVALLTSGCQTSQTVKKDPEKAVKVRTQLAAEYIRSGDLDSAKRSLDQALSVYSRDATANMMMGIL
LQQEGSKPNLEKAEHYFKRAISSEPDNAQARNNYGTYLYQMERYNDAIEQFRIAGATLGYDQRYQALENLGRIYLKLGDI
ASAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGLRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=409194 GQO96_RS16390 WP_187170118.1 3382989..3383789(-) (pilF) [Acinetobacter baumannii strain A1429]
TTGAGTACTCCGAAGTTGAAGATCATGTTATGTATGGGGGTTGCTGTTGCACTTTTGACAAGTGGTTGTCAGACTTCGCA
AACGGTTAAAAAGGACCCGGAAAAAGCAGTAAAAGTGCGTACGCAGCTTGCTGCCGAATACATTCGTTCTGGTGACTTGG
ATTCTGCAAAGCGTTCTCTTGACCAAGCGTTAAGTGTCTATAGTCGTGATGCAACGGCAAATATGATGATGGGTATTTTA
TTGCAACAAGAAGGCAGTAAACCCAATTTAGAAAAAGCGGAACATTACTTTAAGCGTGCAATTTCGTCTGAACCAGACAA
TGCTCAGGCACGTAATAATTATGGTACGTATTTGTACCAAATGGAGCGTTATAATGATGCTATTGAGCAATTTCGTATTG
CTGGTGCAACATTAGGCTATGATCAACGTTACCAGGCTTTAGAAAATCTGGGGCGAATTTATTTAAAACTAGGCGATATT
GCGAGTGCTGAAAAAACATTCAAACAAGCACTTCTTGCTAACCGCGACTCTTACATTTCAATGTTAGAGTTAGCAGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAACAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCACGCGCACTTTGGATTGGTTTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTTTTAGTAAACCAG
TTACGTGCTTTATTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-66)

Tetratricopeptide repeat (TPR_16)

(89-134)

Tetratricopeptide repeat (TPR_16)

(149-206)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

99.248

100

0.992
  pilF Acinetobacter baylyi ADP1

74.231

97.744

0.726

Multiple sequence alignment