Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   GP482_RS07305 Genome accession   NZ_CP046875
Coordinates   1498869..1500230 (-) Length   453 a.a.
NCBI ID   WP_033153501.1    Uniprot ID   A0A6G8HZM5
Organism   Streptococcus ruminicola strain CNU_77-61     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1493869..1505230
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GP482_RS07285 (GP482_07285) gltX 1495185..1496642 (-) 1458 WP_158914576.1 glutamate--tRNA ligase -
  GP482_RS07290 (GP482_07290) - 1496765..1497262 (-) 498 WP_015695763.1 LURP-one-related family protein -
  GP482_RS07295 (GP482_07295) - 1497301..1498227 (-) 927 WP_039696180.1 aldo/keto reductase -
  GP482_RS07300 (GP482_07300) - 1498255..1498752 (-) 498 WP_039696179.1 carbonic anhydrase -
  GP482_RS07305 (GP482_07305) radA 1498869..1500230 (-) 1362 WP_033153501.1 DNA repair protein RadA Machinery gene
  GP482_RS07310 (GP482_07310) - 1500266..1500712 (-) 447 WP_039696177.1 dUTP diphosphatase -
  GP482_RS07315 (GP482_07315) - 1500739..1501506 (-) 768 WP_015695766.1 epoxyqueuosine reductase QueH -
  GP482_RS07320 (GP482_07320) - 1501707..1502723 (+) 1017 WP_074626854.1 NAD(P)H-dependent glycerol-3-phosphate dehydrogenase -
  GP482_RS07325 (GP482_07325) galU 1502790..1503704 (+) 915 WP_074626878.1 UTP--glucose-1-phosphate uridylyltransferase GalU -
  GP482_RS07330 (GP482_07330) - 1503766..1504437 (-) 672 WP_158914578.1 rhomboid family intramembrane serine protease -
  GP482_RS07335 (GP482_07335) - 1504434..1504961 (-) 528 WP_158914580.1 5-formyltetrahydrofolate cyclo-ligase -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 49450.86 Da        Isoelectric Point: 6.3749

>NTDB_id=409136 GP482_RS07305 WP_033153501.1 1498869..1500230(-) (radA) [Streptococcus ruminicola strain CNU_77-61]
MAKKKTTFICQECGYHSPKYLGRCPNCSSWTSFVEEVEVQEVKNARVSLTGEKSKPTKLKDVSSINYSRTKTGMDEFNRV
LGGGVVPGSLVLIGGDPGIGKSTLLLQVSIQLAEKGTVLYVSGEESAEQIKLRSERLGDIDNEFYLYAETNMQAIRAQIE
QIQPDFLIIDSIQTIMSPDISGVQGSVSQVREVTAELMQLAKTNNIATFIVGHVTKEGQLAGPRMLEHMVDTVLYFEGER
HHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNPSQVFLEERLDGATGSAIVVTMEGSRPILAEVQALVTPTVFGNAK
RTTTGLDFNRVSLIMAVLEKRCGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKEKPTNPQEAFIGEIGLTGEIR
RVTRIEQRINEAAKLGFTKVYAPKNSLVGIDMPSSIEVVGVTTVGEVLKKVFK

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=409136 GP482_RS07305 WP_033153501.1 1498869..1500230(-) (radA) [Streptococcus ruminicola strain CNU_77-61]
ATCGCTAAGAAGAAAACAACGTTTATTTGTCAGGAGTGTGGCTATCATTCTCCTAAGTATTTAGGGCGTTGCCCAAACTG
TTCGTCATGGACGTCTTTTGTTGAAGAAGTCGAAGTACAAGAGGTTAAAAATGCGCGTGTCAGTTTGACAGGTGAAAAGA
GCAAACCAACTAAGTTGAAGGACGTTAGCTCGATTAATTATTCACGCACAAAGACTGGCATGGATGAATTTAACCGCGTG
CTCGGTGGTGGTGTGGTGCCAGGTAGTCTAGTGCTTATCGGTGGTGACCCAGGTATCGGGAAATCAACGCTTCTTTTGCA
GGTTTCGATTCAGCTGGCAGAGAAAGGAACGGTTCTTTACGTTTCTGGTGAAGAATCAGCTGAGCAGATTAAACTTCGCA
GTGAACGCCTTGGCGACATTGATAATGAATTTTACCTTTATGCTGAGACAAATATGCAAGCTATCCGTGCGCAGATTGAG
CAAATCCAGCCTGATTTCTTAATTATTGACTCGATTCAAACGATTATGAGTCCTGATATTTCAGGAGTCCAAGGTTCGGT
ATCTCAAGTGCGTGAAGTGACGGCAGAGCTCATGCAGTTGGCTAAGACTAATAACATCGCAACCTTTATTGTTGGTCACG
TGACTAAGGAAGGACAGCTTGCTGGTCCGCGTATGCTTGAGCATATGGTGGATACGGTGCTTTATTTTGAGGGTGAACGC
CATCATACTTTCCGTATCTTGCGTGCAGTGAAAAACCGTTTTGGTTCAACTAACGAAATCGGCATTTTTGAAATGCAATC
TGGTGGACTGGTTGAAGTGCTTAATCCGAGCCAAGTTTTCTTGGAAGAACGTTTGGATGGCGCGACTGGTTCAGCTATCG
TGGTAACCATGGAAGGTAGTCGTCCGATTTTGGCGGAGGTTCAAGCTTTGGTGACACCGACTGTTTTTGGAAATGCCAAA
CGCACAACGACTGGGCTTGATTTCAATCGCGTTAGTCTGATTATGGCGGTACTTGAAAAACGTTGTGGGCTTTTATTGCA
AAATCAAGATGCTTATCTCAAATCTGCTGGTGGTGTCAAACTTGATGAGCCAGCGATTGACTTGGCAGTAGCGGTAGCTA
TTGCGTCAAGCTACAAAGAAAAGCCGACAAATCCACAAGAAGCTTTTATCGGTGAAATTGGCTTGACAGGTGAAATTCGT
CGCGTCACTCGTATTGAACAACGTATCAATGAAGCAGCTAAGCTTGGCTTTACAAAGGTTTACGCACCGAAAAATTCTTT
AGTTGGTATCGATATGCCATCATCTATTGAAGTTGTTGGTGTGACAACTGTTGGAGAAGTTCTTAAGAAAGTTTTTAAAT
AG

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(71-218)

Subunit ChlI of Mg-chelatase (ChlI)

(360-425)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6G8HZM5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

88.496

99.779

0.883
  radA Streptococcus pneumoniae D39

88.496

99.779

0.883
  radA Streptococcus pneumoniae R6

88.496

99.779

0.883
  radA Streptococcus pneumoniae TIGR4

88.496

99.779

0.883
  radA Streptococcus mitis SK321

88.496

99.779

0.883
  radA Streptococcus mitis NCTC 12261

88.274

99.779

0.881
  radA Bacillus subtilis subsp. subtilis str. 168

62.031

100

0.62

Multiple sequence alignment